FastQCFastQC Report
Wed 25 May 2016
SRR2088366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3805552
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT402851.0585849306486943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT384911.011443280764525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT356450.936657809432114No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG199620.5245493951994349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133700.3513287954020862No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA107210.2817199712420169No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT97550.25633600591977196No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96360.2532089956989157No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT96320.25310388611166No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA80030.2102980067017873No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT76710.20157391095956645No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG71910.18896076048888572No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT68350.17960600722313083No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA63030.16562643211812636No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60190.15816365142297362No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52040.13674757301963025No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50140.1317548676249858No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT48840.12833880603917644No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA41790.10981324128536411No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC39410.1035592208436516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97750.028.1805631
ACGTTTA2100.020.26190426
ACGCCTA2150.018.9302336
GTATTGG13200.018.7803021
TATACCG2300.018.55
ATTGGAC13350.017.7378273
TTGGACC21550.017.4269144
GGACCCT20800.016.987986
TGGACCC21550.016.6542915
GTATCAA165750.016.6527922
ACGGACC5450.016.6330288
CGTTTAG3650.016.21917726
TCTAACG2400.016.18752
ATGCGAC2400.016.187521
CAATGCG2400.016.187519
TACGTTA3000.016.03333319
AAGACGG6350.015.4409455
CGCCTAC2650.015.3584897
TATTGGA17500.015.328572
CGCAAGA5950.015.2352932
Produced by FastQC (version 0.11.5)