FastQCFastQC Report
Wed 25 May 2016
SRR2088365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260932
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT298381.3197212476978522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT289271.2794281296385739No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261271.155585395757148No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG141010.6236808537364237No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97450.4310169434551769No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA80650.3567113031263214No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT75680.33472921786236826No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT73100.3233179945261512No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72270.3196469420575232No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA61980.27413473735609917No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT58750.2598485934119204No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG52650.23286856924489546No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT51420.2274283348636757No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46160.20416359271309353No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45440.2009790652704283No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39790.17598937075506915No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT37740.16692231345303618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.16294165414970463No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31610.13980960064256687No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC30080.13304247982690323No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG28210.12477155438553658No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC27420.12127742010816778No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG26140.11561603798787402No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25280.11181229687580166No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24650.10902583536346958No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.10730088299869259No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.10221448499999117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72600.027.3422851
GTATTGG7600.021.4210531
ATTGGAC7800.020.8717963
TCTAACG1153.0540832E-919.3043482
CGTCTGT7250.018.88275734
ATCACGT1351.1532393E-917.81481623
TTGGACC13200.017.3787884
GTATTAG2300.016.8913041
CGAATTA1655.4569682E-1116.81818215
GGACCCT13150.016.3193916
TGGACCC13500.016.170375
GTATCAA123500.016.1481782
CAATGCG1504.6784407E-916.03333319
GTCGACG700.002593533415.85714336
CGACGAG1201.9374183E-615.41666624
TGCGACG1201.9374183E-615.41666622
GCCGTCA1802.0190782E-1015.41666613
GCGTTAT1455.3525582E-815.3103451
TATTGGA10550.015.2559242
TCGACGA855.3670007E-415.23529437
Produced by FastQC (version 0.11.5)