##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2260932
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42373720218034	31.0	31.0	34.0	30.0	34.0
2	31.616508590262775	31.0	31.0	34.0	30.0	34.0
3	31.6948771568539	31.0	31.0	34.0	30.0	34.0
4	35.47821296704191	37.0	35.0	37.0	33.0	37.0
5	35.31887690563007	37.0	35.0	37.0	33.0	37.0
6	35.35464224487954	37.0	35.0	37.0	32.0	37.0
7	35.227797209292454	37.0	35.0	37.0	32.0	37.0
8	35.26889088216718	37.0	35.0	37.0	32.0	37.0
9	36.75402135048732	39.0	37.0	39.0	32.0	39.0
10	36.64102502861652	39.0	35.0	39.0	32.0	39.0
11	36.74672657116623	39.0	35.0	39.0	32.0	39.0
12	36.61559171173658	39.0	35.0	39.0	32.0	39.0
13	36.648385709963854	39.0	35.0	39.0	32.0	39.0
14	37.645113165721035	39.0	36.0	41.0	32.0	41.0
15	37.638083321391356	39.0	36.0	41.0	32.0	41.0
16	37.58608971875315	39.0	36.0	41.0	32.0	41.0
17	37.680988636544576	39.0	36.0	41.0	32.0	41.0
18	37.668233277250266	39.0	37.0	41.0	32.0	41.0
19	37.74199312495909	40.0	37.0	41.0	32.0	41.0
20	37.70799342925838	40.0	37.0	41.0	32.0	41.0
21	37.654216048956805	39.0	37.0	41.0	32.0	41.0
22	37.549039511139654	39.0	36.0	41.0	32.0	41.0
23	37.43367160091502	39.0	36.0	41.0	32.0	41.0
24	37.365558981871196	39.0	36.0	41.0	31.0	41.0
25	37.30882043334342	39.0	36.0	41.0	31.0	41.0
26	37.072555477121824	39.0	36.0	41.0	31.0	41.0
27	36.91043251190217	39.0	35.0	40.0	31.0	41.0
28	36.80139031160601	39.0	35.0	40.0	30.0	41.0
29	36.646019871451244	39.0	35.0	40.0	30.0	41.0
30	36.46091434859606	38.0	35.0	40.0	30.0	41.0
31	36.19449678274269	38.0	35.0	40.0	30.0	41.0
32	36.05380701409861	38.0	35.0	40.0	30.0	41.0
33	36.09201205520555	38.0	35.0	40.0	30.0	41.0
34	36.0345322194564	38.0	35.0	40.0	30.0	41.0
35	35.94507043997785	38.0	35.0	40.0	29.0	41.0
36	35.85610668520769	38.0	35.0	40.0	29.0	41.0
37	35.721207006668045	38.0	35.0	40.0	28.0	41.0
38	35.591702890666326	38.0	35.0	40.0	27.0	41.0
39	35.50323583371813	38.0	34.0	40.0	26.0	41.0
40	35.330581813163775	38.0	34.0	40.0	25.0	41.0
41	35.18034996187413	38.0	34.0	40.0	25.0	41.0
42	34.99401618447614	38.0	34.0	40.0	24.0	41.0
43	34.656343490206694	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	3.0
15	9.0
16	15.0
17	55.0
18	123.0
19	302.0
20	633.0
21	1330.0
22	2499.0
23	4229.0
24	7295.0
25	11685.0
26	17647.0
27	26074.0
28	36900.0
29	50147.0
30	64495.0
31	80139.0
32	96231.0
33	118429.0
34	145463.0
35	176419.0
36	225472.0
37	312117.0
38	404236.0
39	478982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53729523930839	22.409342695844014	13.73791869901439	22.315443365833204
2	17.852151236746614	23.045142445681694	36.53143924717771	22.57126707039398
3	19.804531936387296	26.295173848660642	32.23595402250046	21.664340192451608
4	12.755226605665273	15.818565087317973	35.686743343010754	35.739464964006
5	11.471950505366815	40.459111552227135	34.5173583283354	13.551579614070658
6	31.173604513536894	36.44090136280083	15.741074919546453	16.644419204115824
7	24.68145879663785	35.16355202190955	21.704412162771813	18.450577018680793
8	27.69446405287731	33.382605049599015	20.33692300343398	18.586007894089693
9	25.533717953481133	13.700942797041222	23.597348350149407	37.16799089932824
10	16.425173335597886	27.581103721827986	33.63834029506416	22.355382647509963
11	31.401696291617792	22.194608241203184	25.0717403265556	21.331955140623425
12	20.478855622371658	27.5398817832646	31.703695644097213	20.277566950266525
13	31.024152871470704	21.08617154341661	28.539425334331153	19.350250250781535
14	20.964009532352147	22.68232746495693	28.572774413383506	27.780888589307416
15	23.04925579362847	28.942666121758638	26.912131811129214	21.09594627348368
16	21.69636238506952	28.441810722303902	27.311259250609925	22.550567642016656
17	19.404077610472143	27.082680947503064	28.11283134565745	25.400410096367338
18	20.567624324835954	26.47753227430104	32.24449032522871	20.7103530756343
19	19.870522421726967	24.944359228848988	33.63316543796983	21.551952911454215
20	24.121335803111283	23.983516532120383	32.27514140186437	19.62000626290397
21	23.55900133219398	24.893274101122902	31.128224997478917	20.419499569204206
22	22.057983168003283	24.54094152323024	32.05832815847624	21.342747150290233
23	21.009610196149197	26.27818970229976	33.00223093839178	19.709969163159265
24	19.685731370956756	25.56883621444608	31.61390966203318	23.13152275256399
25	20.85171071045038	26.451171463803426	32.52932861315599	20.167789212590208
26	22.25033747144983	25.56941119856767	31.127605783809507	21.052645546172993
27	20.809869558217585	26.931813959906798	30.909332965343495	21.34898351653212
28	20.658692963786613	26.34435710583069	31.024949003331372	21.972000927051322
29	19.660697446893582	26.279781966021094	32.11662270249614	21.94289788458919
30	19.17169556625321	26.76427243278436	33.67310471964659	20.390927281315847
31	20.481465165692732	26.132762949084714	32.68652042608977	20.699251459132782
32	21.518692291497487	26.34081874200551	30.72414384864295	21.416345117854053
33	22.39877183391628	26.186634538323133	30.304449669428358	21.110143958332227
34	20.817830876824246	28.637128405454032	30.412590913835537	20.132449803886185
35	19.51310344583561	29.542861085605406	30.63917888729073	20.304856581268254
36	20.251427287507983	26.216533712645933	32.562146937634566	20.96989206221151
37	20.5247216634556	26.46368842583501	31.661367966838455	21.350221943870935
38	20.68934404042227	26.075352996021113	32.17018468489986	21.065118278656765
39	21.557481604931063	25.505809108809995	32.27407989271681	20.662629393542133
40	19.86831094433623	26.252315416828104	31.475073111442537	22.40430052739313
41	20.79996213950707	25.75150424692118	31.42584562472467	22.02268798884708
42	18.513692583412507	27.35915985089335	31.828555657578377	22.298591908115768
43	19.114374072285234	27.027172864995496	31.465740676853613	22.39271238586565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	258.5
2	336.0
3	1061.5
4	1787.0
5	1787.0
6	3122.0
7	4457.0
8	4748.5
9	5040.0
10	7672.5
11	10305.0
12	10305.0
13	18303.0
14	26301.0
15	43254.5
16	60208.0
17	58425.0
18	56642.0
19	56642.0
20	60295.5
21	63949.0
22	52002.5
23	40056.0
24	44560.5
25	49065.0
26	49065.0
27	53366.0
28	57667.0
29	60279.0
30	62891.0
31	66345.0
32	69799.0
33	69799.0
34	76207.0
35	82615.0
36	89614.5
37	96614.0
38	100766.0
39	104918.0
40	104918.0
41	109738.5
42	114559.0
43	131378.0
44	148197.0
45	183208.0
46	218219.0
47	218219.0
48	220388.5
49	222558.0
50	202531.0
51	182504.0
52	172055.0
53	161606.0
54	161606.0
55	141598.0
56	121590.0
57	102667.0
58	83744.0
59	73744.5
60	63745.0
61	63745.0
62	55529.5
63	47314.0
64	41352.0
65	35390.0
66	30083.5
67	24777.0
68	24777.0
69	20811.0
70	16845.0
71	14080.5
72	11316.0
73	9061.5
74	6807.0
75	6807.0
76	5311.0
77	3815.0
78	3020.5
79	2226.0
80	1789.5
81	1353.0
82	1353.0
83	986.0
84	619.0
85	515.0
86	411.0
87	335.0
88	259.0
89	259.0
90	222.0
91	185.0
92	109.5
93	34.0
94	27.5
95	21.0
96	21.0
97	11.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2260932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.200818191030855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23293842303457	32.501244947905356
2	13.296757197539982	11.488615804424112
3	4.72748978306731	6.126942798547403
4	2.167233611860451	3.7450506097389793
5	1.1245366684534035	2.4290452081501512
6	0.7094078263888082	1.8388199118670365
7	0.4612496654610045	1.394845405677826
8	0.3190599977801441	1.1026922364904572
9	0.24720195264610065	0.9611393951458812
>10	1.4509741950494854	12.006312485582624
>50	0.15387605002488128	4.6498763642694385
>100	0.09572031814117372	7.745202161830335
>500	0.00585299773869592	1.7425209494591032
>1k	0.006366418592967493	5.108455169110037
>5k	9.241575376888295E-4	2.7678672804027578
>10k+	4.1073668341725755E-4	4.3913692713985135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29838	1.3197212476978522	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28927	1.2794281296385739	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26127	1.155585395757148	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14101	0.6236808537364237	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9745	0.4310169434551769	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8065	0.3567113031263214	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7568	0.33472921786236826	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7310	0.3233179945261512	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7227	0.3196469420575232	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6198	0.27413473735609917	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5875	0.2598485934119204	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5265	0.23286856924489546	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5142	0.2274283348636757	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4616	0.20416359271309353	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4544	0.2009790652704283	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3979	0.17598937075506915	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3774	0.16692231345303618	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3684	0.16294165414970463	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3161	0.13980960064256687	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3008	0.13304247982690323	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2821	0.12477155438553658	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2742	0.12127742010816778	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2614	0.11561603798787402	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2528	0.11181229687580166	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2465	0.10902583536346958	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2426	0.10730088299869259	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2311	0.10221448499999117	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.4229547814794964E-5	0.0
3	0.0	0.0	0.0	4.4229547814794964E-5	0.0
4	0.0	0.0	0.0	4.4229547814794964E-5	0.0
5	0.0	0.0	0.0	4.4229547814794964E-5	0.0
6	0.0	0.0	0.0	4.4229547814794964E-5	0.0
7	0.0	0.0	0.0	4.4229547814794964E-5	0.0
8	0.0	0.0	0.0	4.4229547814794964E-5	0.0
9	0.0	0.0	0.0	4.4229547814794964E-5	0.0
10	4.4229547814794964E-5	0.0	0.0	4.4229547814794964E-5	0.0
11	4.4229547814794964E-5	0.0	0.0	1.3268864344438488E-4	0.0
12	8.845909562958993E-5	0.0	0.0	5.749841215923345E-4	0.0
13	8.845909562958993E-5	0.0	0.0	7.076727650367194E-4	0.0
14	8.845909562958993E-5	0.0	0.0	8.845909562958992E-4	0.0
15	8.845909562958993E-5	0.0	0.0	0.0014595750778882337	0.0
16	8.845909562958993E-5	0.0	0.0	0.0025210842254433127	0.0
17	1.3268864344438488E-4	0.0	0.0	0.003980659303331547	0.0
18	1.3268864344438488E-4	0.0	0.0	0.004467184329294291	0.0
19	1.7691819125917986E-4	0.0	0.0	0.0051306275465162155	0.0
20	1.7691819125917986E-4	0.0	0.0	0.006590202624404449	0.0
21	1.7691819125917986E-4	0.0	0.0	0.009420893684551327	0.0
22	1.7691819125917986E-4	0.0	0.0	0.01596686676114098	0.0
23	2.6537728688876976E-4	0.0	0.0	0.025033924063173948	0.0
24	3.096068347035647E-4	0.0	0.0	0.03870085433794559	0.0
25	3.538363825183597E-4	0.0	0.0	0.04591027063175717	0.0
26	3.538363825183597E-4	0.0	0.0	0.055508082507567676	0.0
27	3.538363825183597E-4	0.0	0.0	0.12627535901123962	0.0
28	3.538363825183597E-4	0.0	0.0	0.20062522888790993	0.0
29	3.538363825183597E-4	0.0	0.0	0.2904554404997585	0.0
30	3.9806593033315466E-4	0.0	0.0	0.4656486793941613	0.0
31	3.9806593033315466E-4	0.0	0.0	0.7165186745996783	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7260	0.0	27.342285	1
GTATTGG	760	0.0	21.421053	1
ATTGGAC	780	0.0	20.871796	3
TCTAACG	115	3.0540832E-9	19.304348	2
CGTCTGT	725	0.0	18.882757	34
ATCACGT	135	1.1532393E-9	17.814816	23
TTGGACC	1320	0.0	17.378788	4
GTATTAG	230	0.0	16.891304	1
CGAATTA	165	5.4569682E-11	16.818182	15
GGACCCT	1315	0.0	16.319391	6
TGGACCC	1350	0.0	16.17037	5
GTATCAA	12350	0.0	16.148178	2
CAATGCG	150	4.6784407E-9	16.033333	19
GTCGACG	70	0.0025935334	15.857143	36
CGACGAG	120	1.9374183E-6	15.416666	24
TGCGACG	120	1.9374183E-6	15.416666	22
GCCGTCA	180	2.0190782E-10	15.416666	13
GCGTTAT	145	5.3525582E-8	15.310345	1
TATTGGA	1055	0.0	15.255924	2
TCGACGA	85	5.3670007E-4	15.235294	37
>>END_MODULE