FastQCFastQC Report
Wed 25 May 2016
SRR2088364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947341
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT461881.1701041283233449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT450011.1400332527643293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT403441.0220550998760938No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG230740.5845453939753369No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157700.39950944192558996No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA131500.3331356475156314No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA115690.2930833692858053No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT114590.2902966832609597No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT111410.2822406272982243No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA91300.23129494006218365No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT88560.22435355850938646No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG86030.2179441806522416No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT79270.20081872835409964No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA74890.18972265127335086No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72300.18316127236030533No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60070.15217839046588577No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.14663035192551138No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT54360.1377129566460055No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC45760.11592613863357637No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA45600.11552080248450794No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC44160.1118727771428919No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43470.11012476500003421No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG42400.10741407950313896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112950.026.6485181
ATTGGAC16700.021.8233533
GTATTGG17250.021.7710131
GAACCGT1251.8189894E-1120.726
TTGGACC25450.018.7544214
TGGACCC25500.017.5568625
TATTGGA22950.017.1699352
GGACCCT26800.017.0503736
CTAGTAC5700.015.903513
GTATCAA192250.015.6756832
GACCCTC29100.015.384887
TATACTG6850.015.1240875
CCCGTAT1601.0988515E-815.0312511
TAGTACT6300.014.9761914
ACGGACC5000.014.7999998
ACGTTTA2301.8189894E-1214.47826126
CGTCTGT10450.014.1626834
ACCCTCG31350.014.0446588
TCGTTTA3100.013.72580730
TAACGGA1356.5762033E-613.70370328
Produced by FastQC (version 0.11.5)