##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3947341
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.412348464447334	31.0	31.0	34.0	30.0	34.0
2	31.603699553699567	31.0	31.0	34.0	30.0	34.0
3	31.686993345647107	31.0	31.0	34.0	30.0	34.0
4	35.471434568232134	37.0	35.0	37.0	33.0	37.0
5	35.30447508842028	37.0	35.0	37.0	33.0	37.0
6	35.334129734421225	37.0	35.0	37.0	32.0	37.0
7	35.20962769621373	37.0	35.0	37.0	32.0	37.0
8	35.2435340650833	37.0	35.0	37.0	32.0	37.0
9	36.74450699850862	39.0	37.0	39.0	32.0	39.0
10	36.61844770948342	39.0	35.0	39.0	32.0	39.0
11	36.721457305056745	39.0	35.0	39.0	32.0	39.0
12	36.58317231777037	39.0	35.0	39.0	32.0	39.0
13	36.620886059755165	39.0	35.0	39.0	32.0	39.0
14	37.62479958027442	39.0	36.0	41.0	32.0	41.0
15	37.61569522369616	39.0	36.0	41.0	32.0	41.0
16	37.57010681367533	39.0	36.0	41.0	32.0	41.0
17	37.63778021711324	39.0	36.0	41.0	32.0	41.0
18	37.616769871161374	39.0	36.0	41.0	32.0	41.0
19	37.67562087997971	40.0	37.0	41.0	32.0	41.0
20	37.640924105619455	39.0	37.0	41.0	32.0	41.0
21	37.587879537136516	39.0	36.0	41.0	32.0	41.0
22	37.480380590377166	39.0	36.0	41.0	32.0	41.0
23	37.36461785287869	39.0	36.0	41.0	31.0	41.0
24	37.29391988176344	39.0	36.0	41.0	31.0	41.0
25	37.23551448937398	39.0	36.0	41.0	31.0	41.0
26	37.00512927563137	39.0	36.0	41.0	31.0	41.0
27	36.84651465378846	39.0	35.0	40.0	31.0	41.0
28	36.72434228509774	39.0	35.0	40.0	30.0	41.0
29	36.57664564576508	38.0	35.0	40.0	30.0	41.0
30	36.39183617528863	38.0	35.0	40.0	30.0	41.0
31	36.15248492592862	38.0	35.0	40.0	30.0	41.0
32	36.01053671319504	38.0	35.0	40.0	30.0	41.0
33	36.06233157966337	38.0	35.0	40.0	30.0	41.0
34	36.02723402918572	38.0	35.0	40.0	30.0	41.0
35	35.94437445358787	38.0	35.0	40.0	29.0	41.0
36	35.861502717905545	38.0	35.0	40.0	29.0	41.0
37	35.727647801393395	38.0	35.0	40.0	28.0	41.0
38	35.602695080055156	38.0	34.0	40.0	27.0	41.0
39	35.533589066665385	38.0	34.0	40.0	27.0	41.0
40	35.358414436452286	38.0	34.0	40.0	26.0	41.0
41	35.21651258404075	38.0	34.0	40.0	25.0	41.0
42	35.03628113203293	38.0	34.0	40.0	24.0	41.0
43	34.69885500137941	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	6.0
15	16.0
16	41.0
17	116.0
18	238.0
19	584.0
20	1265.0
21	2573.0
22	4624.0
23	8175.0
24	13523.0
25	20898.0
26	32072.0
27	46565.0
28	65081.0
29	88159.0
30	112985.0
31	139334.0
32	170134.0
33	207142.0
34	253929.0
35	309903.0
36	394059.0
37	539905.0
38	706823.0
39	829185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.836745292590635	22.005496864851555	13.337079314910975	22.82067852764684
2	18.077308243701264	22.83238260895119	35.891578660166424	23.19873048718112
3	20.049369942956538	26.016272726374538	31.645049160941507	22.28930816972742
4	13.00389300037671	15.651650060129086	35.30845194271283	36.03600499678138
5	12.08400794357518	39.84147809880119	34.18448013485534	13.890033822768288
6	31.919411066842212	35.91625349824097	15.70137973891792	16.4629556959989
7	25.547932139635265	34.32231975904793	21.52915088916818	18.600597212148635
8	27.970676969636017	32.955196928768	20.25340602699387	18.820720074602118
9	25.918662714976993	13.487180357612885	22.880870945783506	37.71328598162662
10	16.57125644832813	27.144297895722712	33.458700426438966	22.825745229510193
11	32.40297709268087	21.732984305130973	24.545991846156692	21.318046756031464
12	20.801065831403974	26.907657585194695	31.366633893550116	20.92464268985122
13	31.12163352494755	20.490730342273444	28.095849839170217	20.291786293608784
14	21.351942991497314	22.066271953702504	27.941746101996255	28.640038952803927
15	23.479273769355117	28.608980070381556	26.140710924138556	21.771035236124774
16	22.512192384696434	27.852420148145296	26.69042274280332	22.944964724354953
17	20.35544940252185	26.60808883752379	27.53339526531911	25.503066494635252
18	21.446284980192996	25.82237004606392	31.351611122525263	21.379733851217818
19	20.644530077335606	24.603144243175343	32.47857228448214	22.273753395006917
20	24.54865186463495	23.68201784441729	31.180964603767446	20.588365687180307
21	24.1310796305665	24.644868533020077	30.173374937711234	21.05067689870219
22	22.66670145801946	24.27740598038021	31.139468315506562	21.91642424609376
23	21.643962353391817	25.80836061541174	32.04681328519629	20.500863746000157
24	20.53739466643495	25.158480100908438	30.60634994544429	23.697775287212327
25	21.69351469761543	25.84420753109498	31.326277613208486	21.136000158081096
26	22.76947950531763	25.281930291809097	30.33773874615849	21.610851456714787
27	21.605252751155778	26.34439233904545	30.103986455692578	21.946368454106192
28	21.435847574354483	25.845626207616725	30.251680815009397	22.466845403019402
29	20.3665454796026	25.897635902244065	31.20209275053764	22.533725867615694
30	20.05408197568946	26.27226783802058	32.53709269100389	21.13655749528607
31	21.285974533236423	25.7794550812813	31.47511198044456	21.459458405037722
32	22.22503705659075	25.682301073051455	29.87502219848754	22.217639671870256
33	22.808366442118885	25.672319670380645	29.598177608673787	21.921136278826683
34	21.581591253453908	28.029577378797526	29.539251866002964	20.849579501745605
35	20.498507729633694	28.857881799418898	29.80054168109621	20.843068789851195
36	21.1284507723047	26.0143980466851	31.351687123053214	21.50546405795699
37	21.323772129137055	26.14240826926278	30.691495870257977	21.842323731342187
38	21.352626996248866	25.624616672337154	31.338868367338925	21.68388796407506
39	22.321279058485192	25.177733567989186	31.125180216251902	21.37580715727372
40	20.781179026590305	25.8895291792627	30.49723852081693	22.83205327333007
41	21.57442187031726	25.602044515535898	30.459263590351078	22.364270023795765
42	19.611024231248326	26.926480382617058	30.91769877494749	22.544796611187127
43	20.014561701155284	26.6366397025238	30.456375570288962	22.892423026031956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	279.0
1	413.0
2	547.0
3	1768.5
4	2990.0
5	2990.0
6	5096.5
7	7203.0
8	7805.0
9	8407.0
10	12410.0
11	16413.0
12	16413.0
13	29497.0
14	42581.0
15	68292.0
16	94003.0
17	91585.5
18	89168.0
19	89168.0
20	94961.5
21	100755.0
22	82507.5
23	64260.0
24	70933.0
25	77606.0
26	77606.0
27	85110.5
28	92615.0
29	96902.5
30	101190.0
31	108576.0
32	115962.0
33	115962.0
34	125909.0
35	135856.0
36	147391.5
37	158927.0
38	168295.0
39	177663.0
40	177663.0
41	186468.0
42	195273.0
43	219533.0
44	243793.0
45	301599.5
46	359406.0
47	359406.0
48	368969.5
49	378533.0
50	349983.0
51	321433.0
52	306666.5
53	291900.0
54	291900.0
55	257303.5
56	222707.0
57	191691.0
58	160675.0
59	144674.0
60	128673.0
61	128673.0
62	114976.5
63	101280.0
64	90213.5
65	79147.0
66	69516.5
67	59886.0
68	59886.0
69	51388.0
70	42890.0
71	37301.0
72	31712.0
73	25128.0
74	18544.0
75	18544.0
76	14512.5
77	10481.0
78	8275.0
79	6069.0
80	4928.0
81	3787.0
82	3787.0
83	2840.5
84	1894.0
85	1507.0
86	1120.0
87	1023.0
88	926.0
89	926.0
90	753.0
91	580.0
92	359.0
93	138.0
94	97.0
95	56.0
96	56.0
97	32.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3947341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.690295407808165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85386342602895	27.08193338719334
2	13.978087097803519	10.53676463904573
3	5.389851270860289	6.094352598086237
4	2.673222906526429	4.030182441516026
5	1.546163094793873	2.913767189571599
6	0.9401616870215899	2.126098302896821
7	0.6189642741772399	1.6330262438433818
8	0.4327852355863916	1.304944270191116
9	0.3366894775767874	1.1420933283512749
>10	1.8878130235995598	13.590893944610984
>50	0.18865085856113561	5.001825624859002
>100	0.1353181850970938	9.79299536210427
>500	0.011232555533125564	2.909054837811603
>1k	0.005986212226416818	4.638334209400561
>5k	6.053473037949591E-4	1.6849661771149704
>10k+	6.053473037949591E-4	5.518767443402953
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46188	1.1701041283233449	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45001	1.1400332527643293	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40344	1.0220550998760938	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23074	0.5845453939753369	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15770	0.39950944192558996	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13150	0.3331356475156314	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11569	0.2930833692858053	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11459	0.2902966832609597	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11141	0.2822406272982243	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9130	0.23129494006218365	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8856	0.22435355850938646	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8603	0.2179441806522416	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7927	0.20081872835409964	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7489	0.18972265127335086	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7230	0.18316127236030533	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6007	0.15217839046588577	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5788	0.14663035192551138	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5436	0.1377129566460055	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4576	0.11592613863357637	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4560	0.11552080248450794	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4416	0.1118727771428919	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4347	0.11012476500003421	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4240	0.10741407950313896	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.5333509316778052E-5	0.0
3	0.0	0.0	0.0	2.5333509316778052E-5	0.0
4	0.0	0.0	0.0	2.5333509316778052E-5	0.0
5	0.0	0.0	0.0	2.5333509316778052E-5	0.0
6	0.0	0.0	0.0	2.5333509316778052E-5	0.0
7	0.0	0.0	0.0	2.5333509316778052E-5	0.0
8	0.0	0.0	0.0	2.5333509316778052E-5	0.0
9	0.0	0.0	0.0	5.0667018633556105E-5	0.0
10	0.0	0.0	0.0	5.0667018633556105E-5	0.0
11	0.0	0.0	0.0	2.786686024845586E-4	0.0
12	0.0	0.0	0.0	4.0533614906844884E-4	0.0
13	0.0	0.0	0.0	5.066701863355611E-4	0.0
14	0.0	0.0	0.0	7.853387888201197E-4	0.0
15	0.0	0.0	0.0	0.0010386738819879002	0.0
16	0.0	0.0	0.0	0.0017226786335409077	0.0
17	0.0	0.0	0.0	0.0027360190062120297	0.0
18	0.0	0.0	0.0	0.0030906881366469224	0.0
19	0.0	0.0	0.0	0.003394690248448259	0.0
20	0.0	0.0	0.0	0.004129362018634823	0.0
21	0.0	0.0	0.0	0.005446704503107282	0.0
22	0.0	0.0	0.0	0.009474732484474993	0.0
23	0.0	0.0	0.0	0.01588411034161984	0.0
24	0.0	0.0	0.0	0.02515617475156061	0.0
25	0.0	0.0	0.0	0.030273543633549775	0.0
26	0.0	0.0	0.0	0.03858293468945297	0.0
27	0.0	0.0	0.0	0.10080203357145988	0.0
28	0.0	0.0	0.0	0.1649464791615419	0.0
29	0.0	0.0	0.0	0.24094700711187608	0.0
30	0.0	0.0	0.0	0.37881196481378226	0.0
31	0.0	0.0	0.0	0.5742093221740914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11295	0.0	26.648518	1
ATTGGAC	1670	0.0	21.823353	3
GTATTGG	1725	0.0	21.771013	1
GAACCGT	125	1.8189894E-11	20.72	6
TTGGACC	2545	0.0	18.754421	4
TGGACCC	2550	0.0	17.556862	5
TATTGGA	2295	0.0	17.169935	2
GGACCCT	2680	0.0	17.050373	6
CTAGTAC	570	0.0	15.90351	3
GTATCAA	19225	0.0	15.675683	2
GACCCTC	2910	0.0	15.38488	7
TATACTG	685	0.0	15.124087	5
CCCGTAT	160	1.0988515E-8	15.031251	1
TAGTACT	630	0.0	14.976191	4
ACGGACC	500	0.0	14.799999	8
ACGTTTA	230	1.8189894E-12	14.478261	26
CGTCTGT	1045	0.0	14.16268	34
ACCCTCG	3135	0.0	14.044658	8
TCGTTTA	310	0.0	13.725807	30
TAACGGA	135	6.5762033E-6	13.703703	28
>>END_MODULE