FastQCFastQC Report
Wed 25 May 2016
SRR2088363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314462
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT463321.3978739234301072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT461751.3931371064142537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT404531.2204997372122535No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG195550.5899901703504218No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154240.46535455829633887No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA117210.353632052502035No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT103860.3133540224627707No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT100880.30436312137535443No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93880.28324355506263155No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA87780.26483936156154453No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT82130.24779285446627536No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT77640.23424616121711458No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG74850.2258285055010436No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65680.19816187363137666No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60000.181024854109053No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59530.17960682608519873No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT58310.17592598738498133No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56390.17013319205349164No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46080.1390270879557527No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC45480.13721683941466217No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG40700.12279519270397428No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG39800.12007981989233849No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39310.11860145025044788No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38240.11537317368550311No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35830.10810200871212282No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35150.10605039369888689No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34220.10324450846019655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG250.00549781929.63
GGTATCA110150.027.4434871
GTATTGG9750.021.2512821
ATTGGAC10650.019.9765263
TTGGACC19600.017.0841834
GGACCCT19250.016.9142866
TGGACCC19800.016.8181825
TATTGGA12800.016.6210942
CGTCTGT7600.016.5526334
GTCGTCA2150.016.34883924
TATACCG2400.016.18755
GTATCAA190300.015.894642
TATACTG4400.015.5568185
CTAGTAC4650.015.5161293
TAGTACT5500.015.1363644
GACCCTC20650.015.0508487
TAGGTCG2400.014.64583421
CCTAGTA3000.014.1833332
TACTGTG6550.014.1221377
TTTTTAC22250.013.8853931
Produced by FastQC (version 0.11.5)