##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3314462
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311805656543957	31.0	31.0	34.0	30.0	34.0
2	31.503406586046243	31.0	31.0	34.0	30.0	34.0
3	31.57926505116064	31.0	31.0	34.0	30.0	34.0
4	35.38472065753054	37.0	35.0	37.0	33.0	37.0
5	35.219207823170095	37.0	35.0	37.0	33.0	37.0
6	35.25301693004777	37.0	35.0	37.0	32.0	37.0
7	35.121420912353194	37.0	35.0	37.0	32.0	37.0
8	35.16571347024042	37.0	35.0	37.0	32.0	37.0
9	36.62871410201716	39.0	35.0	39.0	32.0	39.0
10	36.50264115262145	38.0	35.0	39.0	32.0	39.0
11	36.61608158428125	39.0	35.0	39.0	32.0	39.0
12	36.48083942431683	38.0	35.0	39.0	32.0	39.0
13	36.51242916648313	38.0	35.0	39.0	32.0	39.0
14	37.4793529085565	39.0	36.0	41.0	32.0	41.0
15	37.468548440139	39.0	36.0	41.0	32.0	41.0
16	37.41903934937254	39.0	36.0	41.0	32.0	41.0
17	37.51731412217126	39.0	36.0	41.0	32.0	41.0
18	37.504520794023286	39.0	36.0	41.0	32.0	41.0
19	37.58015780539949	39.0	36.0	41.0	32.0	41.0
20	37.541926563044015	39.0	36.0	41.0	32.0	41.0
21	37.49504534974304	39.0	36.0	41.0	32.0	41.0
22	37.39088123502396	39.0	36.0	41.0	32.0	41.0
23	37.27999536576373	39.0	36.0	41.0	31.0	41.0
24	37.20766145455884	39.0	36.0	41.0	31.0	41.0
25	37.149019056486395	39.0	36.0	41.0	31.0	41.0
26	36.895941483112495	39.0	35.0	40.0	31.0	41.0
27	36.729170827724076	39.0	35.0	40.0	30.0	41.0
28	36.60886110626702	38.0	35.0	40.0	30.0	41.0
29	36.45363440582514	38.0	35.0	40.0	30.0	41.0
30	36.2478966420493	38.0	35.0	40.0	30.0	41.0
31	35.975031543580826	38.0	35.0	40.0	30.0	41.0
32	35.82661680839907	38.0	34.0	40.0	29.0	41.0
33	35.85330681118082	38.0	35.0	40.0	29.0	41.0
34	35.801423277744625	38.0	35.0	40.0	29.0	41.0
35	35.70645069999294	38.0	35.0	40.0	28.0	41.0
36	35.601969188363	38.0	34.0	40.0	27.0	41.0
37	35.449014953256366	38.0	34.0	40.0	27.0	41.0
38	35.318314405173446	38.0	34.0	40.0	26.0	41.0
39	35.20945631598733	38.0	34.0	40.0	25.0	41.0
40	35.01502747655577	38.0	34.0	40.0	24.0	41.0
41	34.847392427488984	38.0	34.0	40.0	24.0	41.0
42	34.642394451950274	38.0	33.0	40.0	23.0	41.0
43	34.28981264531016	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	7.0
15	12.0
16	38.0
17	89.0
18	211.0
19	611.0
20	1177.0
21	2383.0
22	4381.0
23	7446.0
24	12213.0
25	19566.0
26	29456.0
27	42663.0
28	59916.0
29	79984.0
30	101540.0
31	122768.0
32	149394.0
33	181366.0
34	220372.0
35	265896.0
36	336236.0
37	458285.0
38	581158.0
39	637292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.305406427951205	22.532978202797317	13.974515321038528	22.18710004821295
2	17.89264743418389	23.37664453537256	35.98556869863042	22.745139331813128
3	20.053148897166416	26.291174857337328	32.33040535688748	21.32527088860877
4	13.048392167416612	16.05476243203271	35.2654216581756	35.631423742375084
5	11.595999592090662	40.52524964835922	34.069239593031995	13.809511166518126
6	31.19537952162372	36.32616696163661	15.709216156347546	16.769237360392125
7	24.56443911560911	35.328659673877695	21.578192780608134	18.528708429905063
8	28.094514283162695	33.03803151159977	20.279761843701934	18.5876923615356
9	25.776068634970017	13.500411228126918	23.577099390489316	37.146420746413746
10	16.48614465937458	27.983365022739736	33.295629878996955	22.234860438888724
11	31.721467918473646	22.08080828804192	24.914511012647	21.28321278083743
12	20.66241821447945	27.662920860157698	31.592367026684876	20.082293898677975
13	31.119861986651227	21.085231932060168	28.473369132003928	19.321536949284678
14	21.109489262510778	22.512793931564158	28.85225415165418	27.52546265427089
15	23.09798694328069	28.927681174199616	26.916223507766873	21.058108374752827
16	21.7461235036033	28.191694459010243	27.458694653913668	22.603487383472793
17	19.582604959718953	27.026437473110263	28.206146276529946	25.184811290640834
18	20.57959934372456	26.249056407947958	32.47661309738956	20.69473115093792
19	19.99356154935552	24.78767293153459	34.000570831706625	21.218194687403265
20	24.10047241452761	23.87419134689129	32.44303298695233	19.582303251628773
21	23.426637565915676	24.780281083325136	31.460098199949194	20.332983150809998
22	21.930618000749444	24.568512174826562	32.28563187630451	21.215237948119483
23	20.985064846119823	26.23774235456614	33.1126137514927	19.664579047821334
24	19.620620179081854	25.663290150860078	31.77034462908309	22.945745040974977
25	20.90327178287155	26.324875651010633	32.70431219304973	20.067540373068088
26	22.187522439539205	25.37205133140763	31.44709458126236	20.993331647790804
27	20.67484858779494	26.790893967105372	31.23478259820146	21.29947484689823
28	20.54674333270377	26.512990645238954	31.150606041040746	21.789659981016527
29	19.616637632291457	26.410621090240287	32.08297455212942	21.889766725338834
30	19.188694877177653	26.78084708770232	33.87213369771625	20.158324337403776
31	20.5280374311125	26.173176823267248	32.66801670980087	20.630769035819387
32	21.494981689336008	26.384734536102695	30.85354425544779	21.266739519113507
33	22.2680181579997	26.313863305719	30.378565209074655	21.039553327206647
34	20.663836242503308	28.746173587146266	30.531350185942696	20.058639984407726
35	19.426772731140076	29.673443231510877	30.75174794582047	20.148036091528578
36	20.31095846022673	26.365666584803204	32.616122918289605	20.70725203668046
37	20.346378990014067	26.63810899023733	31.679530493938383	21.335981525810222
38	20.627540759254444	26.196227321357128	32.21587696585449	20.960354953533937
39	21.617203636668634	25.610159356179075	32.16654769310977	20.60608931404252
40	19.781521103575784	26.48167334547809	31.513892752428603	22.222912798517527
41	20.785213407183427	25.73518718875039	31.52137511306511	21.958224291001073
42	18.40757263169709	27.36435053411383	31.91057854939957	22.317498284789508
43	19.04405601874452	27.08388269348087	31.510483451009545	22.36157783676506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	526.0
2	672.0
3	1764.5
4	2857.0
5	2857.0
6	4946.0
7	7035.0
8	7581.5
9	8128.0
10	12227.0
11	16326.0
12	16326.0
13	29250.5
14	42175.0
15	68697.5
16	95220.0
17	91792.5
18	88365.0
19	88365.0
20	93289.0
21	98213.0
22	78472.0
23	58731.0
24	64645.5
25	70560.0
26	70560.0
27	75569.5
28	80579.0
29	83771.0
30	86963.0
31	92907.5
32	98852.0
33	98852.0
34	107753.0
35	116654.0
36	129771.5
37	142889.0
38	148834.0
39	154779.0
40	154779.0
41	162408.0
42	170037.0
43	191768.5
44	213500.0
45	262644.5
46	311789.0
47	311789.0
48	318741.0
49	325693.0
50	296441.5
51	267190.0
52	250167.5
53	233145.0
54	233145.0
55	204843.0
56	176541.0
57	148807.5
58	121074.0
59	106510.5
60	91947.0
61	91947.0
62	81024.5
63	70102.0
64	61225.0
65	52348.0
66	44993.0
67	37638.0
68	37638.0
69	32281.0
70	26924.0
71	23103.0
72	19282.0
73	15659.5
74	12037.0
75	12037.0
76	9541.5
77	7046.0
78	5532.0
79	4018.0
80	3136.5
81	2255.0
82	2255.0
83	1707.5
84	1160.0
85	915.0
86	670.0
87	550.0
88	430.0
89	430.0
90	312.0
91	194.0
92	120.0
93	46.0
94	29.5
95	13.0
96	13.0
97	8.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3314462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.39002857607505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.03796346124237	26.561114838020277
2	14.112143230764893	10.55306877335927
3	5.651510424455467	6.3393040880512785
4	2.8537774955378614	4.268112884316821
5	1.672586046559037	3.1269020038393394
6	0.9882879926789748	2.217126977259523
7	0.6631069568829412	1.7355511646793138
8	0.45188748553020774	1.3516868797716135
9	0.34649308083891295	1.1659847574581315
>10	1.9077912534619763	13.297959643726037
>50	0.1729910311223956	4.5241423045168245
>100	0.12239538957872263	8.64637321706721
>500	0.010502540995772079	2.64380260176393
>1k	0.00710941236630882	5.360041461836978
>5k	8.078877688987294E-4	2.1633913203901094
>10k+	6.463102151189836E-4	6.045437083943565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	46332	1.3978739234301072	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46175	1.3931371064142537	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40453	1.2204997372122535	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19555	0.5899901703504218	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15424	0.46535455829633887	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11721	0.353632052502035	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10386	0.3133540224627707	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10088	0.30436312137535443	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9388	0.28324355506263155	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8778	0.26483936156154453	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8213	0.24779285446627536	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7764	0.23424616121711458	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7485	0.2258285055010436	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6568	0.19816187363137666	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6000	0.181024854109053	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5953	0.17960682608519873	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5831	0.17592598738498133	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5639	0.17013319205349164	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4608	0.1390270879557527	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4548	0.13721683941466217	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4070	0.12279519270397428	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3980	0.12007981989233849	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3931	0.11860145025044788	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3824	0.11537317368550311	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3583	0.10810200871212282	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3515	0.10605039369888689	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3422	0.10324450846019655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.05124270545265E-5	0.0
10	0.0	0.0	0.0	1.2068323607270199E-4	0.0
11	0.0	0.0	0.0	1.508540450908775E-4	0.0
12	0.0	0.0	0.0	2.4136647214540398E-4	0.0
13	0.0	0.0	0.0	3.01708090181755E-4	0.0
14	0.0	0.0	0.0	3.922205172362815E-4	0.0
15	0.0	0.0	0.0	7.24099416436212E-4	0.0
16	0.0	0.0	0.0	0.0013576864058178974	0.0
17	0.0	0.0	0.0	0.0021119566312722848	0.0
18	0.0	0.0	0.0	0.002353323103417689	0.0
19	0.0	0.0	0.0	0.002866226856726672	0.0
20	0.0	0.0	0.0	0.0039523759813809905	0.0
21	0.0	0.0	0.0	0.0054307456232715896	0.0
22	0.0	3.0170809018175498E-5	0.0	0.008327143289016438	0.0
23	0.0	3.0170809018175498E-5	0.0	0.01421045104756066	0.0
24	0.0	3.0170809018175498E-5	0.0	0.022416911100504394	0.0
25	0.0	3.0170809018175498E-5	0.0	0.02688219083519437	0.0
26	0.0	3.0170809018175498E-5	0.0	0.03533001736028351	0.0
27	0.0	3.0170809018175498E-5	0.0	0.10381775383154189	0.0
28	0.0	3.0170809018175498E-5	0.0	0.17456830097916343	0.0
29	0.0	3.0170809018175498E-5	0.0	0.25421923678714675	0.0
30	0.0	3.0170809018175498E-5	0.0	0.3979529709497348	0.0
31	0.0	3.0170809018175498E-5	0.0	0.61168298203449	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	0.005497819	29.6	3
GGTATCA	11015	0.0	27.443487	1
GTATTGG	975	0.0	21.251282	1
ATTGGAC	1065	0.0	19.976526	3
TTGGACC	1960	0.0	17.084183	4
GGACCCT	1925	0.0	16.914286	6
TGGACCC	1980	0.0	16.818182	5
TATTGGA	1280	0.0	16.621094	2
CGTCTGT	760	0.0	16.55263	34
GTCGTCA	215	0.0	16.348839	24
TATACCG	240	0.0	16.1875	5
GTATCAA	19030	0.0	15.89464	2
TATACTG	440	0.0	15.556818	5
CTAGTAC	465	0.0	15.516129	3
TAGTACT	550	0.0	15.136364	4
GACCCTC	2065	0.0	15.050848	7
TAGGTCG	240	0.0	14.645834	21
CCTAGTA	300	0.0	14.183333	2
TACTGTG	655	0.0	14.122137	7
TTTTTAC	2225	0.0	13.885393	1
>>END_MODULE