FastQCFastQC Report
Wed 25 May 2016
SRR2088362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578374
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117542.032249029174894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104081.7995276412840135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58271.0074795893314705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36360.6286589646145919No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT21580.37311497404793437No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT21500.3717317860069782No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG15990.27646470968612No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG14950.25848326515368947No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14530.2512215279386694No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.2460345727850837No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA13970.24153921165197606No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11920.2060950181024735No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT11890.2055763225871149No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.19209023918779197No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10410.17998734382942524No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8090.1398748906416955No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT8000.13831880409561978No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7610.13157576239595833No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.1300196758498826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7250.12535141621165544No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6880.11895417152223303No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT6520.11272982533793013No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6420.11100084028673489No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.10667837765874677No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA5990.10356620456659531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.030.2381921
CGCGATA358.864048E-426.4285714
TGCGGTC358.864048E-426.4285712
CGTCTGT1250.025.1634
ACGTGCG400.001930033723.1259
TACTACG400.001930033723.1255
CCTCCGT652.6790203E-622.769238
GTATCAA34850.021.0746062
ACTACGT450.003823544420.5555556
GAACCGC450.003823544420.5555556
GCGCGAT555.138847E-420.18181813
GTGTTAC609.229255E-418.51
AGCCGTC500.00703075918.512
AACCCGT500.00703075918.56
ACCGACC609.229255E-418.58
TGCGCGA500.00703075918.512
CGTAGTC500.00703075918.526
CTACGTG500.00703075918.57
GACGGCG500.00703075918.535
GCGATAT500.00703075918.515
Produced by FastQC (version 0.11.5)