##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	578374
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.48453249973201	31.0	30.0	31.0	27.0	34.0
2	30.665292699879316	31.0	30.0	31.0	27.0	34.0
3	30.568127197972245	31.0	30.0	33.0	27.0	34.0
4	34.4021377171173	35.0	35.0	37.0	30.0	37.0
5	34.10912662049124	35.0	33.0	37.0	30.0	37.0
6	34.41462963411218	35.0	35.0	37.0	30.0	37.0
7	34.28182456334483	35.0	35.0	37.0	30.0	37.0
8	34.412172746354436	35.0	35.0	37.0	30.0	37.0
9	35.73741385331982	37.0	35.0	39.0	30.0	39.0
10	35.44844166577336	37.0	35.0	39.0	30.0	39.0
11	35.75768274507499	37.0	35.0	39.0	30.0	39.0
12	35.46669283197377	37.0	35.0	39.0	30.0	39.0
13	35.635706307683265	37.0	35.0	39.0	30.0	39.0
14	36.3507159727097	38.0	35.0	40.0	30.0	41.0
15	36.42414423884891	38.0	35.0	40.0	30.0	41.0
16	36.24157552033805	38.0	34.0	40.0	30.0	41.0
17	36.35230490997175	38.0	35.0	40.0	30.0	41.0
18	36.35351174153748	38.0	35.0	40.0	30.0	41.0
19	36.4527693153565	38.0	35.0	40.0	30.0	41.0
20	36.43292229595383	38.0	35.0	40.0	30.0	41.0
21	36.427536507519356	38.0	35.0	40.0	30.0	41.0
22	36.27951636830148	38.0	35.0	40.0	30.0	41.0
23	36.12659282747841	38.0	34.0	40.0	29.0	41.0
24	36.01777915328144	38.0	34.0	40.0	29.0	41.0
25	35.92200029738543	38.0	34.0	40.0	29.0	41.0
26	35.618736319405784	38.0	34.0	40.0	27.0	41.0
27	35.38240999768316	38.0	34.0	40.0	27.0	41.0
28	35.20920200423947	37.0	33.0	40.0	27.0	41.0
29	35.009760120614	37.0	33.0	40.0	27.0	41.0
30	34.76991185634209	37.0	33.0	40.0	26.0	41.0
31	34.46641446538053	37.0	33.0	39.0	25.0	40.0
32	34.31953545629644	36.0	33.0	39.0	25.0	40.0
33	34.351303827627106	36.0	33.0	39.0	25.0	40.0
34	34.28911569330572	36.0	33.0	39.0	25.0	40.0
35	34.14472123574019	36.0	33.0	39.0	24.0	40.0
36	34.000228226026756	36.0	33.0	39.0	24.0	40.0
37	33.81619332819248	36.0	33.0	39.0	23.0	40.0
38	33.66147510088628	36.0	32.0	39.0	22.0	40.0
39	33.49176311521611	36.0	32.0	39.0	21.0	41.0
40	33.253787341754645	36.0	31.0	39.0	19.0	41.0
41	33.0423065352177	36.0	31.0	39.0	18.0	41.0
42	32.790941501519775	36.0	31.0	39.0	16.0	41.0
43	32.37122000643183	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	5.0
15	12.0
16	22.0
17	51.0
18	154.0
19	263.0
20	478.0
21	997.0
22	1734.0
23	2715.0
24	4275.0
25	6230.0
26	8737.0
27	11929.0
28	15938.0
29	20014.0
30	24893.0
31	29447.0
32	34299.0
33	41629.0
34	48520.0
35	58683.0
36	73022.0
37	85126.0
38	76074.0
39	33123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.46356509801616	26.028659656208614	15.327106681835629	19.180668563939594
2	23.842185160467103	25.816340291921836	29.6486356578961	20.69283888971496
3	25.9833602478673	24.584265544440104	31.007445009630448	18.424929198062152
4	19.151967412089753	19.151794513584637	30.85806070120718	30.83817737311843
5	11.72874299328808	42.66097715319153	32.992146949897474	12.618132903622914
6	32.70167746129667	36.772572764335884	13.602789890278608	16.922959884088844
7	23.09733840041219	36.84847520808335	21.593121405872324	18.461064985632134
8	28.017684059103626	35.24812664469703	19.731523201250404	17.002666094948943
9	25.055932666406168	13.253880706947408	23.975835704924496	37.71435092172193
10	17.52412798638943	29.612845667336362	32.604681399924615	20.258344946349595
11	32.961198117481075	21.77639382129902	23.15543229813235	22.10697576308755
12	18.642435517502516	29.662294639800546	31.709585838920834	19.985684003776104
13	31.459228803507767	20.67382005415181	29.362834428933525	18.504116713406894
14	20.53895922015858	23.179983885859322	29.864412992285267	26.416643901696823
15	24.244347083375118	29.60575682862646	26.41180274355348	19.738093344444945
16	21.50442447274601	27.39905320778597	29.093631456462425	22.002890863005597
17	19.210752903830393	28.01267000245516	30.033161933281928	22.743415160432523
18	19.879697220137835	25.151026844221906	33.521735071078574	21.447540864561685
19	19.58784454349608	25.153793220303815	35.67968131347536	19.578680922724743
20	22.441707268998954	24.095654368972326	34.83801139055352	18.624626971475205
21	22.443263355545028	24.033929602644655	33.567207377925016	19.955599663885305
22	21.39463392199511	24.273394032235196	33.935481193829595	20.396490851940094
23	20.502304737073242	26.164557881232557	34.567771027051705	18.7653663546425
24	19.449525739400457	25.354182587737345	33.80234934488757	21.39394232797463
25	20.16255917451338	26.145711944174533	34.402134259147196	19.289594622164895
26	20.851041021899324	25.344500271450656	33.49372551324921	20.31073319340081
27	21.135977758336303	25.984051841887773	33.156054732750775	19.723915667025143
28	20.356205500247246	26.515887643635434	33.452575669030765	19.675331187086556
29	19.468544574963605	25.68009626988765	34.14935664466245	20.702002510486295
30	19.1718507401785	26.285932631826466	35.12000885240346	19.42220777559157
31	20.07472673391266	25.40259416917081	34.14849215213685	20.374186944779677
32	20.46426706594695	26.47439200240675	32.92782870599301	20.1335122256533
33	21.052986475878928	26.39433999453641	32.35449726301666	20.198176266567998
34	20.79052654510749	27.672405744379937	32.338936397555905	19.19813131295667
35	19.47718950021958	28.120558669649743	32.582896188279555	19.81935564185112
36	20.161003087967302	25.76741001497301	34.141576211932076	19.930010685127616
37	19.946090246103733	26.03695878445435	33.5786186792629	20.43833229017902
38	20.544146175312168	25.892415634174426	33.22953659742658	20.333901593086825
39	21.414344351578738	25.83120956336212	32.782939758702845	19.9715063263563
40	20.25125610763969	26.093496595628434	32.39962377285286	21.255623523879013
41	20.274943202841065	26.108711664078953	32.2706414880337	21.345703645046285
42	19.03785439871087	27.11273328330803	31.849633628067654	21.999778689913445
43	19.415637632397033	26.553406619246374	31.876778693371417	22.154177054985183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	77.0
2	103.0
3	200.0
4	297.0
5	297.0
6	718.0
7	1139.0
8	1239.0
9	1339.0
10	2123.5
11	2908.0
12	2908.0
13	5964.0
14	9020.0
15	15141.0
16	21262.0
17	18675.0
18	16088.0
19	16088.0
20	19457.5
21	22827.0
22	17540.5
23	12254.0
24	12793.5
25	13333.0
26	13333.0
27	14102.0
28	14871.0
29	15268.0
30	15665.0
31	16603.5
32	17542.0
33	17542.0
34	18979.0
35	20416.0
36	22287.5
37	24159.0
38	25130.5
39	26102.0
40	26102.0
41	27967.5
42	29833.0
43	33017.0
44	36201.0
45	43329.0
46	50457.0
47	50457.0
48	52125.5
49	53794.0
50	48529.5
51	43265.0
52	40560.5
53	37856.0
54	37856.0
55	33324.5
56	28793.0
57	25119.5
58	21446.0
59	19116.0
60	16786.0
61	16786.0
62	14923.5
63	13061.0
64	11279.0
65	9497.0
66	8065.5
67	6634.0
68	6634.0
69	5500.5
70	4367.0
71	3643.0
72	2919.0
73	2356.0
74	1793.0
75	1793.0
76	1402.0
77	1011.0
78	783.5
79	556.0
80	439.0
81	322.0
82	322.0
83	252.0
84	182.0
85	137.0
86	92.0
87	80.5
88	69.0
89	69.0
90	58.5
91	48.0
92	27.0
93	6.0
94	5.0
95	4.0
96	4.0
97	4.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	578374.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.953797589856826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.25839633033652	48.557894474337786
2	8.501911588020832	9.684323034219933
3	2.3808832007365655	4.068010196995225
4	1.05109980806461	2.394565028609966
5	0.6170648506181181	1.7572093300959772
6	0.3823643139501507	1.306625984538081
7	0.2762510303269615	1.1013481685661817
8	0.22524629899645346	1.0262905696726705
9	0.15735975143518593	0.8066011888826786
>10	1.010405562169561	10.720380806989677
>50	0.08353230556961151	3.2482836819963543
>100	0.045729362452297855	5.155617073917563
>500	0.005182661077927074	1.867572062339852
>1k	0.003658348996183817	3.4455280778237283
>5k	3.048624163486514E-4	1.011746226037032
>10k+	6.097248326973028E-4	3.8480040949772962
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11754	2.032249029174894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10408	1.7995276412840135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5827	1.0074795893314705	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3636	0.6286589646145919	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2158	0.37311497404793437	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2150	0.3717317860069782	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1599	0.27646470968612	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1495	0.25848326515368947	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1453	0.2512215279386694	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.2460345727850837	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1397	0.24153921165197606	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1192	0.2060950181024735	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1189	0.2055763225871149	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1111	0.19209023918779197	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1041	0.17998734382942524	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	809	0.1398748906416955	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	800	0.13831880409561978	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	761	0.13157576239595833	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.1300196758498826	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	725	0.12535141621165544	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	688	0.11895417152223303	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	652	0.11272982533793013	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	642	0.11100084028673489	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	617	0.10667837765874677	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	599	0.10356620456659531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.7289850511952474E-4	0.0
13	0.0	0.0	0.0	1.7289850511952474E-4	0.0
14	0.0	0.0	0.0	5.186955153585742E-4	0.0
15	0.0	0.0	0.0	6.915940204780989E-4	0.0
16	0.0	0.0	0.0	6.915940204780989E-4	0.0
17	0.0	0.0	0.0	0.0015560865460757225	0.0
18	0.0	0.0	0.0	0.0015560865460757225	0.0
19	0.0	0.0	0.0	0.0017289850511952474	0.0
20	0.0	0.0	0.0	0.002593477576792871	0.0
21	0.0	0.0	0.0	0.004668259638227168	0.0
22	0.0	1.7289850511952474E-4	0.0	0.007953331235498138	0.0
23	0.0	1.7289850511952474E-4	0.0	0.010719707317410534	0.0
24	0.0	1.7289850511952474E-4	0.0	0.017635647522191523	0.0
25	0.0	1.7289850511952474E-4	0.0	0.021785211645060115	0.0
26	0.0	1.7289850511952474E-4	0.0	0.028355354839602057	0.0
27	0.0	1.7289850511952474E-4	0.0	0.07866881982938376	0.0
28	0.0	1.7289850511952474E-4	0.0	0.12759909677820924	0.0
29	0.0	1.7289850511952474E-4	0.0	0.18552009599325003	0.0
30	0.0	1.7289850511952474E-4	0.0	0.29444615421855064	0.0
31	0.0	1.7289850511952474E-4	0.0	0.4502277073312424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2435	0.0	30.238192	1
CGCGATA	35	8.864048E-4	26.42857	14
TGCGGTC	35	8.864048E-4	26.42857	12
CGTCTGT	125	0.0	25.16	34
ACGTGCG	40	0.0019300337	23.125	9
TACTACG	40	0.0019300337	23.125	5
CCTCCGT	65	2.6790203E-6	22.76923	8
GTATCAA	3485	0.0	21.074606	2
ACTACGT	45	0.0038235444	20.555555	6
GAACCGC	45	0.0038235444	20.555555	6
GCGCGAT	55	5.138847E-4	20.181818	13
GTGTTAC	60	9.229255E-4	18.5	1
AGCCGTC	50	0.007030759	18.5	12
AACCCGT	50	0.007030759	18.5	6
ACCGACC	60	9.229255E-4	18.5	8
TGCGCGA	50	0.007030759	18.5	12
CGTAGTC	50	0.007030759	18.5	26
CTACGTG	50	0.007030759	18.5	7
GACGGCG	50	0.007030759	18.5	35
GCGATAT	50	0.007030759	18.5	15
>>END_MODULE