FastQCFastQC Report
Wed 25 May 2016
SRR2088361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500551
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51051.0198760965416112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50551.0098871044109392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46100.9209850744479584No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20560.4107473564132326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.3532107617405619No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA11620.23214417711681729No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA10940.21855914781910335No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT10330.20637257741968348No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT10120.20217720072480128No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA8500.169812866221424No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT8490.16961308637881053No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG7570.15123334085837406No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT7540.15063400133053373No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6870.13724875187543328No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA6610.13205447596748382No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA6440.12865821864305535No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.12266482336465215No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT5640.11267583123398014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAT303.5966E-430.8333348
TAAAGGT250.00549341629.64
TCAATCG250.00549341629.632
TGACCCG405.9335325E-527.75000213
GGTATCA11950.026.4728031
TCGATGA358.862371E-426.42857228
CAACCGT400.001929672323.1250026
TAGACAG502.69915E-422.25
TCTAGAC450.00382283620.5555573
ACCCGTT450.00382283620.55555730
GTGCTAG555.137521E-420.1818181
TAGTGTA555.137521E-420.1818189
GCGTTAT656.8921836E-519.9230771
CTATTCA656.8921836E-519.9230774
ATTGGAC1800.019.5277793
TCATTCG609.226885E-418.512
GACCCGC609.226885E-418.514
CGTTATT701.2174529E-418.52
ACCCGCC609.226885E-418.515
CGTCTGT1258.543793E-917.7634
Produced by FastQC (version 0.11.5)