##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500551
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42446024481022	31.0	31.0	34.0	30.0	34.0
2	31.631110516211134	31.0	31.0	34.0	30.0	34.0
3	31.73233696466494	31.0	31.0	34.0	30.0	34.0
4	35.4990440534531	37.0	35.0	37.0	33.0	37.0
5	35.320912354585246	37.0	35.0	37.0	33.0	37.0
6	35.34328569915952	37.0	35.0	37.0	32.0	37.0
7	35.215726269650844	37.0	35.0	37.0	32.0	37.0
8	35.25066976192236	37.0	35.0	37.0	32.0	37.0
9	36.768219422196736	39.0	37.0	39.0	32.0	39.0
10	36.63726573316205	39.0	35.0	39.0	32.0	39.0
11	36.74460944039668	39.0	35.0	39.0	32.0	39.0
12	36.603199274399614	39.0	35.0	39.0	32.0	39.0
13	36.636670389231064	39.0	35.0	39.0	32.0	39.0
14	37.64532884760993	39.0	36.0	41.0	32.0	41.0
15	37.651128456441	39.0	36.0	41.0	32.0	41.0
16	37.60452581255456	39.0	36.0	41.0	32.0	41.0
17	37.65748944662981	39.0	36.0	41.0	32.0	41.0
18	37.63500822094052	39.0	36.0	41.0	32.0	41.0
19	37.702673653633695	40.0	37.0	41.0	32.0	41.0
20	37.65794894026782	39.0	37.0	41.0	32.0	41.0
21	37.6196950960042	39.0	37.0	41.0	32.0	41.0
22	37.50683746511344	39.0	36.0	41.0	32.0	41.0
23	37.40638816024741	39.0	36.0	41.0	32.0	41.0
24	37.32733128092842	39.0	36.0	41.0	31.0	41.0
25	37.27206218746941	39.0	36.0	41.0	31.0	41.0
26	37.05075606681437	39.0	36.0	41.0	31.0	41.0
27	36.898004399152136	39.0	35.0	40.0	31.0	41.0
28	36.79659015764627	39.0	35.0	40.0	30.0	41.0
29	36.6472067781305	39.0	35.0	40.0	30.0	41.0
30	36.48175910147018	38.0	35.0	40.0	30.0	41.0
31	36.247207577249874	38.0	35.0	40.0	30.0	41.0
32	36.08514816672027	38.0	35.0	40.0	30.0	41.0
33	36.16119036821423	38.0	35.0	40.0	30.0	41.0
34	36.13801191087422	38.0	35.0	40.0	30.0	41.0
35	36.07346504152424	38.0	35.0	40.0	30.0	41.0
36	35.998209972610184	38.0	35.0	40.0	29.0	41.0
37	35.869216123831535	38.0	35.0	40.0	29.0	41.0
38	35.76758412229723	38.0	35.0	40.0	28.0	41.0
39	35.70176065975295	38.0	35.0	40.0	27.0	41.0
40	35.56639183619651	38.0	35.0	40.0	26.0	41.0
41	35.45164029239778	38.0	34.0	40.0	26.0	41.0
42	35.30306202564773	38.0	34.0	40.0	25.0	41.0
43	34.97860757445295	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	4.0
17	10.0
18	29.0
19	62.0
20	166.0
21	310.0
22	531.0
23	966.0
24	1581.0
25	2606.0
26	3924.0
27	5471.0
28	8162.0
29	10728.0
30	14001.0
31	17169.0
32	21202.0
33	26085.0
34	31615.0
35	38711.0
36	49706.0
37	67752.0
38	90556.0
39	109199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.897331141082525	22.387728722947312	13.609402438512758	23.1055376974574
2	17.824956897498957	23.060187673184153	36.12359180183438	22.991263627482514
3	19.454361293854173	26.148983819830544	31.811343899023274	22.585310987292004
4	13.005268194449716	16.1577941108898	36.346745886033595	34.49019180862689
5	12.05711306140633	39.68426793673372	34.6106590537228	13.647959948137153
6	31.223791381897147	37.056563666839146	16.083276229594986	15.63636872166872
7	24.96309067407717	34.41527436764685	22.44326751919385	18.178367439082134
8	27.079758106566565	33.812738362324716	20.652041450321747	18.455462080786972
9	25.759413126734337	13.930848205277785	22.959298852664364	37.35043981532352
10	16.19035822523579	27.423778995546904	34.153962333508474	22.23190044570883
11	32.397098397565884	22.380336868770616	24.661423111730873	20.56114162193263
12	20.192148252625604	27.197428433865877	31.787570097752276	20.822853215756236
13	30.05787622040511	21.236597269808673	28.080255558374674	20.625270951411544
14	20.820256077802263	22.435875665017154	28.35135680480111	28.392511452379477
15	23.101741880447747	29.205415631973565	26.043100503245427	21.649741984333264
16	22.037514658845954	28.31080149675058	26.922531370429788	22.72915247397368
17	20.149595146148943	27.32868379046291	27.88726823041009	24.634452832978056
18	20.997061238515155	26.60867723768407	31.147275702176202	21.24698582162457
19	20.666026039304686	25.57321831341861	32.125797371296834	21.63495827597987
20	23.582412181775684	24.90235760192268	31.093934484198414	20.42129573210322
21	23.241387990434543	25.654129149677058	30.278033606965128	20.82644925292328
22	22.297428234086038	25.343071934727927	30.921324700180403	21.438175131005632
23	21.32070458354893	26.512583133387007	31.53444903716105	20.632263245903015
24	20.367554954440205	26.298818701790626	30.55073309213247	22.782893251636697
25	21.298928580704064	26.801664565648654	31.195023084560813	20.70438376908647
26	22.296229555030354	26.23548849168217	30.17554654770443	21.292735405583045
27	21.34627640340345	27.062177480416583	30.048886127487506	21.54265998869246
28	21.04560774027022	26.66241801534709	30.287223479725345	22.004750764657345
29	20.20853019971991	26.607278778785776	31.146876142490974	22.03731487900334
30	20.00635299899511	27.065773517583626	32.02490855077704	20.902964932644228
31	21.113133327073566	26.475823642346136	31.295312565552763	21.11573046502754
32	21.53327033608963	26.561129635142073	30.156767242498766	21.74883278626953
33	22.168170675915142	26.49520228707964	29.817740849583757	21.51888618742146
34	21.173466839542822	28.17475142393083	29.815343491472397	20.83643824505395
35	20.448066231013424	28.56751859450885	30.078253764351686	20.906161410126042
36	20.839035383007925	26.4842143957359	31.101925677902955	21.574824543353223
37	21.13490932991843	26.321393824005945	30.776284534443043	21.76741231163258
38	21.0883606265895	26.02532009725283	31.287920711376067	21.59839856478161
39	21.535667694200992	25.77579507382864	31.237775970880087	21.45076126109028
40	20.44846579069865	26.221104343014	30.89555310048327	22.434876765804084
41	20.895373298624914	25.795173718562143	30.991447424937718	22.318005557875225
42	19.265968902269698	27.06417527884272	31.17284752203072	22.497008296856862
43	19.6375594095307	26.792474692888433	30.72713869316014	22.84282720442073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	79.0
2	99.0
3	266.0
4	433.0
5	433.0
6	664.0
7	895.0
8	910.0
9	925.0
10	1442.5
11	1960.0
12	1960.0
13	3436.0
14	4912.0
15	7948.0
16	10984.0
17	10783.5
18	10583.0
19	10583.0
20	11497.5
21	12412.0
22	10566.5
23	8721.0
24	9658.5
25	10596.0
26	10596.0
27	11782.0
28	12968.0
29	13743.5
30	14519.0
31	15736.5
32	16954.0
33	16954.0
34	18532.5
35	20111.0
36	21780.0
37	23449.0
38	24544.0
39	25639.0
40	25639.0
41	27003.0
42	28367.0
43	30743.5
44	33120.0
45	38468.5
46	43817.0
47	43817.0
48	44597.0
49	45377.0
50	41532.0
51	37687.0
52	35771.0
53	33855.0
54	33855.0
55	30265.5
56	26676.0
57	23351.0
58	20026.0
59	17958.5
60	15891.0
61	15891.0
62	14215.5
63	12540.0
64	10907.5
65	9275.0
66	7931.0
67	6587.0
68	6587.0
69	5521.5
70	4456.0
71	3684.5
72	2913.0
73	2279.5
74	1646.0
75	1646.0
76	1268.0
77	890.0
78	689.5
79	489.0
80	394.0
81	299.0
82	299.0
83	232.5
84	166.0
85	134.5
86	103.0
87	92.0
88	81.0
89	81.0
90	70.0
91	59.0
92	34.0
93	9.0
94	5.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	500551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.128998670615545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.45643963231396	52.23866584679258
2	8.61352920624383	10.530728307955748
3	2.2764959796636073	4.174797591435548
4	0.9995505571355248	2.4440609871340206
5	0.5761017743990134	1.7608262300688275
6	0.3792200202427437	1.3908804067973688
7	0.26067911221650986	1.1154537172898191
8	0.19041184608882245	0.9311748389146465
9	0.1632484039073216	0.8981290318887695
>10	0.9694283241704722	11.318047245629295
>50	0.07496007962111965	3.179350461647459
>100	0.03404300537456251	4.160028684772428
>500	0.002946029311260217	1.2766225593481524
>1k	0.0022913561309801686	2.5482426792004262
>5k	6.546731802800482E-4	2.032991411124957
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5105	1.0198760965416112	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5055	1.0098871044109392	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4610	0.9209850744479584	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2056	0.4107473564132326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1768	0.3532107617405619	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1162	0.23214417711681729	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1094	0.21855914781910335	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1033	0.20637257741968348	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1012	0.20217720072480128	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	850	0.169812866221424	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	849	0.16961308637881053	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	757	0.15123334085837406	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	754	0.15063400133053373	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	687	0.13724875187543328	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	661	0.13205447596748382	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	644	0.12865821864305535	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	614	0.12266482336465215	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	564	0.11267583123398014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.9977984261343999E-4	0.0
8	0.0	0.0	0.0	1.9977984261343999E-4	0.0
9	0.0	0.0	0.0	1.9977984261343999E-4	0.0
10	0.0	0.0	0.0	1.9977984261343999E-4	0.0
11	0.0	0.0	0.0	3.9955968522687997E-4	0.0
12	0.0	0.0	0.0	9.988992130671999E-4	0.0
13	0.0	0.0	0.0	9.988992130671999E-4	0.0
14	0.0	0.0	0.0	0.00139845889829408	0.0
15	0.0	0.0	0.0	0.0015982387409075199	0.0
16	0.0	0.0	0.0	0.00279691779658816	0.0
17	0.0	0.0	0.0	0.0035960371670419196	0.0
18	0.0	0.0	0.0	0.00439515653749568	0.0
19	0.0	0.0	0.0	0.00479471622272256	0.0
20	0.0	0.0	0.0	0.006592734806243519	0.0
21	0.0	0.0	0.0	0.01018877197328544	0.0
22	0.0	0.0	0.0	0.01518326803862144	0.0
23	0.0	0.0	0.0	0.02157622300225152	0.0
24	0.0	0.0	0.0	0.03416235308689824	0.0
25	0.0	0.0	0.0	0.03935662899484768	0.0
26	0.0	0.0	1.9977984261343999E-4	0.047947162227225594	0.0
27	0.0	0.0	1.9977984261343999E-4	0.10927957390955167	0.0
28	0.0	0.0	1.9977984261343999E-4	0.1724100041753987	0.0
29	0.0	0.0	3.9955968522687997E-4	0.2533208404338419	0.0
30	0.0	0.0	3.9955968522687997E-4	0.4059526401905101	0.0
31	0.0	0.0	3.9955968522687997E-4	0.6532800853459487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAT	30	3.5966E-4	30.833334	8
TAAAGGT	25	0.005493416	29.6	4
TCAATCG	25	0.005493416	29.6	32
TGACCCG	40	5.9335325E-5	27.750002	13
GGTATCA	1195	0.0	26.472803	1
TCGATGA	35	8.862371E-4	26.428572	28
CAACCGT	40	0.0019296723	23.125002	6
TAGACAG	50	2.69915E-4	22.2	5
TCTAGAC	45	0.003822836	20.555557	3
ACCCGTT	45	0.003822836	20.555557	30
GTGCTAG	55	5.137521E-4	20.181818	1
TAGTGTA	55	5.137521E-4	20.181818	9
GCGTTAT	65	6.8921836E-5	19.923077	1
CTATTCA	65	6.8921836E-5	19.923077	4
ATTGGAC	180	0.0	19.527779	3
TCATTCG	60	9.226885E-4	18.5	12
GACCCGC	60	9.226885E-4	18.5	14
CGTTATT	70	1.2174529E-4	18.5	2
ACCCGCC	60	9.226885E-4	18.5	15
CGTCTGT	125	8.543793E-9	17.76	34
>>END_MODULE