FastQCFastQC Report
Wed 25 May 2016
SRR2088360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891011
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62630.7029093916910116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61200.6868602071130434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46940.5268172895733049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.27261167370548733No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG11690.13119927812339016No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10530.11818035916503836No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG10080.11312991646567774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC502.7014417E-422.27
TATACCG603.7250767E-521.5833325
CAATGCG656.899905E-519.92307719
ATACCGA953.6045458E-617.5263166
CTACGCA650.001579557217.0769239
TAGACTG1107.802755E-716.8181825
TAACACA1655.4569682E-1116.8181824
ATCGTCA1005.8748064E-616.65000210
GGTATCA24050.016.3846151
CGCCGGA803.381497E-416.187514
CTTAACG700.002591880315.85714330
TACGACG700.002591880315.8571432
GCGCAAG1659.731593E-1015.696971
ATGCGAC957.0568276E-515.57894821
ACGGACC1455.3400072E-815.3103458
AAGACGG1701.4842954E-915.2352935
ACCGACC855.3624716E-415.2352938
CCGTATA855.3624716E-415.2352932
CGTCAGA1101.4510611E-515.1363641
GTACTAG1252.9567527E-614.8000011
Produced by FastQC (version 0.11.5)