##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891011
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13414200273622	31.0	31.0	33.0	30.0	34.0
2	31.354062968919575	31.0	31.0	34.0	30.0	34.0
3	31.436149497593185	31.0	31.0	34.0	28.0	34.0
4	35.24418665987289	37.0	35.0	37.0	33.0	37.0
5	35.02248569321815	35.0	35.0	37.0	32.0	37.0
6	35.06923932476703	37.0	35.0	37.0	32.0	37.0
7	34.95455836123235	37.0	35.0	37.0	32.0	37.0
8	34.985599504383224	37.0	35.0	37.0	32.0	37.0
9	36.49709487312727	38.0	35.0	39.0	32.0	39.0
10	36.28388987341346	38.0	35.0	39.0	32.0	39.0
11	36.42568273567891	38.0	35.0	39.0	32.0	39.0
12	36.26971159727545	38.0	35.0	39.0	32.0	39.0
13	36.3302102892108	38.0	35.0	39.0	32.0	39.0
14	37.31272902354741	39.0	36.0	41.0	32.0	41.0
15	37.31573347579323	39.0	36.0	41.0	32.0	41.0
16	37.26239743392618	39.0	36.0	41.0	32.0	41.0
17	37.27669579836837	39.0	36.0	41.0	32.0	41.0
18	37.23170421016127	39.0	36.0	40.0	31.0	41.0
19	37.27495620143859	39.0	36.0	41.0	31.0	41.0
20	37.238907263771154	39.0	36.0	40.0	31.0	41.0
21	37.19158012639575	39.0	36.0	40.0	31.0	41.0
22	37.09622327894942	39.0	36.0	40.0	31.0	41.0
23	36.9968215880612	39.0	36.0	40.0	31.0	41.0
24	36.92402675163382	39.0	36.0	40.0	31.0	41.0
25	36.85007816962978	39.0	35.0	40.0	30.0	41.0
26	36.621636545452304	39.0	35.0	40.0	30.0	41.0
27	36.451459072895844	38.0	35.0	40.0	30.0	41.0
28	36.35798323477488	38.0	35.0	40.0	30.0	41.0
29	36.21935868356283	38.0	35.0	40.0	30.0	41.0
30	36.033086011283814	38.0	35.0	40.0	29.0	41.0
31	35.86352918201908	38.0	34.0	40.0	29.0	41.0
32	35.68573115258959	38.0	34.0	40.0	27.0	41.0
33	35.83703231497703	38.0	34.0	40.0	29.0	41.0
34	35.84354065213561	38.0	34.0	40.0	29.0	41.0
35	35.80464663174753	38.0	34.0	40.0	28.0	41.0
36	35.747738243411135	38.0	34.0	40.0	28.0	41.0
37	35.62045586418125	38.0	34.0	40.0	27.0	41.0
38	35.54281260276248	38.0	34.0	40.0	27.0	41.0
39	35.515988018105276	38.0	34.0	40.0	27.0	41.0
40	35.42737407282289	38.0	34.0	40.0	26.0	41.0
41	35.33350878945378	38.0	34.0	40.0	26.0	41.0
42	35.23350104544164	38.0	34.0	40.0	25.0	41.0
43	34.89077912618363	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	1.0
16	12.0
17	30.0
18	78.0
19	188.0
20	383.0
21	672.0
22	1286.0
23	2201.0
24	3588.0
25	5460.0
26	8352.0
27	11655.0
28	16114.0
29	21421.0
30	27291.0
31	33864.0
32	41460.0
33	50027.0
34	60377.0
35	73990.0
36	92659.0
37	121245.0
38	168504.0
39	150150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.391096181753085	23.33192295044618	13.811950694211408	23.465030173589327
2	18.551061659171435	24.383874048693002	34.330440364933764	22.734623927201795
3	20.00805826190698	26.453433234830996	31.567287048083582	21.971221455178444
4	14.57748557537449	17.925929085050576	36.3206514846618	31.17593385491313
5	12.836990789114838	39.41589946700994	34.64873048705347	13.098379256821746
6	31.12441933937965	38.34935820096497	16.077354825024607	14.448867634630775
7	24.997558952695307	33.73201902108953	23.44898098901136	17.821441037203805
8	25.973977874571695	34.82022107471176	21.14384670896319	18.061954341753356
9	25.822801289770837	14.109365653173755	22.264708292041288	37.803124765014125
10	15.845034460854018	28.23332147414566	35.015841555266995	20.905802509733327
11	32.85907805852004	23.516095760882862	23.57961910683482	20.045207073762278
12	19.596952226179027	27.747693350587145	31.899942873881464	20.755411549352367
13	29.02725106648515	21.8064647911193	27.280134588686334	21.886149553709213
14	20.78706099026836	22.987146062169828	28.22557746200664	28.000215485555174
15	23.39645638493801	29.70726511793906	24.912262587106106	21.984015910016826
16	21.93452157156309	28.663282495951233	27.145680580823356	22.256515351662326
17	20.69570409343992	28.571925599122793	28.302905351336854	22.429464956100432
18	21.114890837486854	27.206398125275673	30.277291750606892	21.40141928663058
19	21.1642729438806	27.37732755263403	30.94271563426265	20.515683869222716
20	22.60174116817862	26.579020909955098	29.97841777486473	20.84082014700155
21	22.295459876477395	27.23165033877247	29.56405700939719	20.90883277535294
22	22.01667543947269	27.09708409884951	29.67359549994332	21.21264496173448
23	21.376616001373723	27.33221026451974	29.919383711312207	21.371790022794332
24	21.030043400137597	27.61357603890412	29.636671152208	21.719709408750283
25	21.74462492606713	27.404936639390538	29.58077958633507	21.269658848207264
26	21.68413184573479	27.593149803986705	29.35160171984409	21.37111663043442
27	21.58491870470735	27.789555908961844	29.19705817324365	21.428467213087156
28	21.15260080964208	28.08809318852405	29.5924517205736	21.166854281260274
29	20.963938716805966	27.608413364144774	30.055857896254928	21.371790022794332
30	20.857767188059405	28.27552072870032	30.078304308252086	20.788407774988187
31	21.64339160795995	27.747468886467168	29.382690000460148	21.226449505112733
32	21.1398063548037	28.16452322137437	29.40042266593791	21.295247757884024
33	21.449005680064555	27.85756853731323	29.409962391036697	21.283463391585514
34	21.366515115974998	28.014581189233358	29.406258733057168	21.21264496173448
35	20.913546521872345	28.41524964338263	29.830495919803457	20.840707914941568
36	21.28649365720513	27.524127087095447	29.706255029399188	21.483124226300237
37	21.04003205347633	27.272951736847244	30.069437975513207	21.617578234163215
38	20.996037085961902	27.19921527343658	30.251702840930133	21.553044799671383
39	21.315786224861423	26.784405579729093	30.313879402162264	21.585928793247223
40	20.708274084158333	26.890577108475654	30.63160836398204	21.769540443383974
41	20.69951998347944	26.428629949574134	30.652820223319356	22.21902984362707
42	20.02219950146519	27.298091718284063	30.44171171848608	22.23799706176467
43	20.089987665696608	26.64748246654643	30.57077858746974	22.691751280287225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	176.5
2	182.0
3	442.0
4	702.0
5	702.0
6	1137.5
7	1573.0
8	1683.5
9	1794.0
10	2538.0
11	3282.0
12	3282.0
13	5580.0
14	7878.0
15	11688.0
16	15498.0
17	15058.0
18	14618.0
19	14618.0
20	16867.0
21	19116.0
22	17482.5
23	15849.0
24	18606.0
25	21363.0
26	21363.0
27	23807.0
28	26251.0
29	28287.5
30	30324.0
31	33217.0
32	36110.0
33	36110.0
34	39403.5
35	42697.0
36	46208.0
37	49719.0
38	51602.5
39	53486.0
40	53486.0
41	55172.5
42	56859.0
43	58966.0
44	61073.0
45	63889.0
46	66705.0
47	66705.0
48	68276.5
49	69848.0
50	65965.0
51	62082.0
52	56349.0
53	50616.0
54	50616.0
55	46613.0
56	42610.0
57	39882.0
58	37154.0
59	33528.0
60	29902.0
61	29902.0
62	26623.5
63	23345.0
64	20084.5
65	16824.0
66	14223.5
67	11623.0
68	11623.0
69	9905.0
70	8187.0
71	6926.5
72	5666.0
73	4574.0
74	3482.0
75	3482.0
76	2665.5
77	1849.0
78	1433.0
79	1017.0
80	845.5
81	674.0
82	674.0
83	526.0
84	378.0
85	305.0
86	232.0
87	196.0
88	160.0
89	160.0
90	125.5
91	91.0
92	52.0
93	13.0
94	9.0
95	5.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	891011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.40089750331421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.79370723590257	48.58619619935939
2	10.875603674548136	12.920412383170026
3	3.179220442310705	5.665456429024183
4	1.2859456045550728	3.055452922040532
5	0.6727551182971683	1.9981128913400061
6	0.40092354021554166	1.428913087140556
7	0.2884625928167205	1.1994455836612392
8	0.20072148224916606	0.9538428955036812
9	0.1560215593186509	0.8341038588054994
>10	0.998861261760722	11.50558661347965
>50	0.10136248319787672	4.096493398304572
>100	0.04281547387839077	4.317720070654637
>500	0.0022733879679279925	0.8796814417233287
>1k	9.472449866366635E-4	1.1650686921020372
>5k	3.7889799465466544E-4	1.3935135336906717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6263	0.7029093916910116	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6120	0.6868602071130434	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4694	0.5268172895733049	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2429	0.27261167370548733	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	1169	0.13119927812339016	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	1053	0.11818035916503836	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1008	0.11312991646567774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.1223205998579142E-4	0.0
13	0.0	0.0	0.0	1.1223205998579142E-4	0.0
14	0.0	0.0	0.0	1.1223205998579142E-4	0.0
15	0.0	0.0	0.0	1.1223205998579142E-4	0.0
16	0.0	0.0	0.0	2.2446411997158284E-4	0.0
17	0.0	0.0	0.0	6.733923599147485E-4	0.0
18	0.0	0.0	0.0	7.8562441990054E-4	0.0
19	0.0	0.0	0.0	8.978564798863313E-4	0.0
20	0.0	0.0	0.0	0.0012345526598437056	0.0
21	0.0	0.0	0.0	0.0020201770797442457	0.0
22	0.0	0.0	0.0	0.0032547297395879513	0.0
23	0.0	0.0	0.0	0.0056116029992895715	0.0
24	0.0	0.0	0.0	0.010886509818621768	0.0
25	0.0	0.0	0.0	0.014477935738167092	0.0
26	0.0	0.0	0.0	0.0222219478771867	0.0
27	0.0	0.0	0.0	0.0745220878305655	0.0
28	0.0	0.0	0.0	0.13624972082275078	0.0
29	0.0	0.0	0.0	0.20067092325459507	0.0
30	0.0	0.0	0.0	0.3087503970209122	0.0
31	0.0	0.0	0.0	0.5053809661160188	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGAC	50	2.7014417E-4	22.2	7
TATACCG	60	3.7250767E-5	21.583332	5
CAATGCG	65	6.899905E-5	19.923077	19
ATACCGA	95	3.6045458E-6	17.526316	6
CTACGCA	65	0.0015795572	17.076923	9
TAGACTG	110	7.802755E-7	16.818182	5
TAACACA	165	5.4569682E-11	16.818182	4
ATCGTCA	100	5.8748064E-6	16.650002	10
GGTATCA	2405	0.0	16.384615	1
CGCCGGA	80	3.381497E-4	16.1875	14
CTTAACG	70	0.0025918803	15.857143	30
TACGACG	70	0.0025918803	15.857143	2
GCGCAAG	165	9.731593E-10	15.69697	1
ATGCGAC	95	7.0568276E-5	15.578948	21
ACGGACC	145	5.3400072E-8	15.310345	8
AAGACGG	170	1.4842954E-9	15.235293	5
ACCGACC	85	5.3624716E-4	15.235293	8
CCGTATA	85	5.3624716E-4	15.235293	2
CGTCAGA	110	1.4510611E-5	15.136364	1
GTACTAG	125	2.9567527E-6	14.800001	1
>>END_MODULE