FastQCFastQC Report
Wed 25 May 2016
SRR2088359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802939
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT281871.5633917730993672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278331.5437571653838538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224551.245466430089981No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG106820.592477061065294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90420.5015144716487913No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT64420.3573054884275064No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63750.353589333859881No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT61940.3435501700279377No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58640.32524672215754385No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA52140.2891944763522227No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT48080.2666756889722836No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG44730.24809491613415652No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43370.24055167701181238No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36100.20022862670339928No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34280.19013399787790936No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT32920.1825907587555652No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32890.1824243637749253No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA32780.18181424884591216No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28320.15707686172410715No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC25080.13910620381499317No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.13117470973782253No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22410.12429705053803818No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG22270.1235205406283851No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21580.11969345607366638No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20750.1150898616092946No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20180.11192835697713566No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.10765755247404377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG250.005497161829.630
GGTATCA64350.027.9153081
GTATTGG5150.021.5533961
ATTGGAC5600.019.8214283
AGTACCG1254.129106E-1019.245
ACGTTTA1002.8771683E-718.526
CGCATAC609.2395936E-418.537
CGTCTGT4550.018.29670334
TCACGTT1054.801004E-717.61904724
GCGCTTA650.001580404817.07692318
GTATCAA106950.016.8480592
TACACCG1107.813669E-716.8181825
GGACCCT9050.016.762436
ACGGACC1452.9831426E-916.5862088
ATCACGT1151.2430773E-616.08695623
TCTACGC700.002593260715.8571422
TATTGGA6800.015.7794122
CGACGAG957.063236E-515.57894824
TGCGACG855.366252E-415.23529422
CGGACCA1601.0975782E-815.031259
Produced by FastQC (version 0.11.5)