##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1802939
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.109726396733333	31.0	31.0	33.0	30.0	34.0
2	31.302809468318117	31.0	31.0	34.0	28.0	34.0
3	31.35812969823161	31.0	31.0	34.0	28.0	34.0
4	35.18507448116659	37.0	35.0	37.0	32.0	37.0
5	35.008354137328	35.0	35.0	37.0	32.0	37.0
6	35.07279891332985	37.0	35.0	37.0	32.0	37.0
7	34.922143233908635	36.0	35.0	37.0	32.0	37.0
8	34.987668467984776	37.0	35.0	37.0	32.0	37.0
9	36.413351755106525	38.0	35.0	39.0	32.0	39.0
10	36.25776856565863	38.0	35.0	39.0	32.0	39.0
11	36.40725504301587	38.0	35.0	39.0	32.0	39.0
12	36.2347683421347	38.0	35.0	39.0	32.0	39.0
13	36.2954387253257	38.0	35.0	39.0	32.0	39.0
14	37.18959931534012	39.0	36.0	41.0	31.0	41.0
15	37.18402452883875	39.0	36.0	41.0	31.0	41.0
16	37.128959992545504	39.0	36.0	41.0	31.0	41.0
17	37.23136667408049	39.0	36.0	40.0	32.0	41.0
18	37.22087214265153	39.0	36.0	40.0	31.0	41.0
19	37.30832878982594	39.0	36.0	41.0	31.0	41.0
20	37.277019910268734	39.0	36.0	41.0	31.0	41.0
21	37.23589650010344	39.0	36.0	41.0	31.0	41.0
22	37.12321049131446	39.0	36.0	40.0	31.0	41.0
23	36.99787901864678	39.0	36.0	40.0	31.0	41.0
24	36.92807965216793	39.0	35.0	40.0	31.0	41.0
25	36.858103907009614	39.0	35.0	40.0	31.0	41.0
26	36.598297002838144	38.0	35.0	40.0	30.0	41.0
27	36.42000478108245	38.0	35.0	40.0	30.0	41.0
28	36.29359895148976	38.0	35.0	40.0	30.0	41.0
29	36.119972444991205	38.0	35.0	40.0	30.0	41.0
30	35.90883329940725	38.0	35.0	40.0	29.0	41.0
31	35.62227562884823	38.0	34.0	40.0	28.0	41.0
32	35.480267496570875	38.0	34.0	40.0	27.0	41.0
33	35.51074717447457	38.0	34.0	40.0	28.0	41.0
34	35.4427021657416	38.0	34.0	40.0	27.0	41.0
35	35.33520768034859	38.0	34.0	40.0	27.0	41.0
36	35.2306411919649	38.0	34.0	40.0	26.0	41.0
37	35.068419397439406	38.0	34.0	40.0	25.0	41.0
38	34.93729848874532	38.0	33.0	40.0	25.0	41.0
39	34.820034399388994	38.0	33.0	40.0	24.0	41.0
40	34.61767369833367	38.0	33.0	40.0	24.0	41.0
41	34.43837312299529	38.0	33.0	40.0	23.0	41.0
42	34.219302483334154	38.0	33.0	40.0	22.0	41.0
43	33.84861218266397	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	14.0
16	33.0
17	89.0
18	159.0
19	405.0
20	910.0
21	1682.0
22	2920.0
23	5068.0
24	8234.0
25	12609.0
26	18656.0
27	26850.0
28	36672.0
29	48551.0
30	60568.0
31	72590.0
32	86989.0
33	105136.0
34	125793.0
35	150467.0
36	191131.0
37	254395.0
38	316740.0
39	276275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22271413508721	23.781392493034982	14.592673407142447	21.403219964735356
2	18.425082601241638	24.17924289174509	35.351889331807676	22.043785175205592
3	20.544011749704232	26.480762798963248	32.78402652557851	20.191198925754005
4	13.453810694649126	16.778216012854568	35.252717923346275	34.51525536915004
5	11.156395196953419	41.19085559744396	34.26777056794489	13.384978637657735
6	30.898993254902134	36.947062546209274	15.40362707778799	16.750317121100604
7	23.712782296017778	36.01924413416094	21.778496111071977	18.4894774587493
8	27.836937356172335	33.53086266368413	20.412448785011584	18.21975119513195
9	25.19242192886171	13.541278989472188	24.128658817630548	37.137640264035554
10	16.385801183512033	28.351153311343314	33.56780234938619	21.695243155758458
11	31.331675669559534	22.195204607587943	25.021312423770297	21.45180729908222
12	20.00383817755343	28.250317953075506	31.845115114820853	19.900728754550208
13	30.966716012022594	21.05734026497846	29.19044959369119	18.785494129307757
14	20.6405208384754	22.889848186766162	29.297275171262033	27.1723558034964
15	22.891789461540295	29.354903299557	27.28722380513151	20.466083433771193
16	21.250025652559515	28.424588962799074	27.973048450335813	22.352336934305598
17	19.067533621492462	27.34418635350392	28.82205110655435	24.766228918449265
18	20.01604047613369	26.08829250462717	33.34472214534158	20.550944873897564
19	19.42317516011357	24.855527558059368	34.90900135833769	20.81229592348937
20	23.6503841782778	23.88372540612855	33.413720597313606	19.052169818280042
21	23.14870331164837	24.691961292090305	32.3225577792704	19.836777616990926
22	21.53356269956998	24.44536393078191	33.14793234823807	20.873141021410042
23	20.47240644303551	26.301943659768856	34.13415539849102	19.091494498704613
24	19.25273123494472	25.484334189897716	32.789794884907366	22.473139690250196
25	20.35875867125843	26.377431515985844	33.595701241140155	19.668108571615566
26	21.604613356303236	25.539632788463724	32.313794310290035	20.541959544943005
27	20.42886642310139	26.853265695622536	32.09143515116152	20.62643273011455
28	20.103065051008382	26.51132401040745	32.18334064546832	21.202270293115852
29	19.211631674726654	26.27177070327948	33.20561594152658	21.310981680467282
30	18.746668633825102	26.70095882334344	34.71382004604704	19.838552496784416
31	19.957746767916163	26.21547373482963	33.57867348812134	20.248106009132865
32	21.00964037052834	26.422801880707002	31.68698441821936	20.880573330545293
33	21.810166622387115	26.384420105172723	31.16478150397767	20.640631768462494
34	20.277779780680323	28.92177716495123	31.305828982566798	19.494614071801653
35	19.07535418558254	29.768672151414993	31.537062540662774	19.618911122339693
36	19.896125160085838	26.5883094214502	33.32797171729049	20.187593701173473
37	20.068066640080445	26.861030794719063	32.41346490369336	20.657437661507128
38	20.260696562667952	26.520032014394275	32.77526305659814	20.444008366339627
39	21.363396099368863	26.113418146703797	32.482019635717016	20.04116611821032
40	19.614917642804333	26.812332530385106	31.803349974680227	21.769399852130327
41	20.43485664240443	26.412651786887963	31.687261743187094	21.46522982752051
42	18.327852467554365	27.96949869074883	32.041128402014714	21.661520439682096
43	18.89847632116228	27.657175312087652	31.52902011659851	21.915328250151557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	254.0
2	322.0
3	851.5
4	1381.0
5	1381.0
6	2446.0
7	3511.0
8	3835.0
9	4159.0
10	6431.0
11	8703.0
12	8703.0
13	16160.5
14	23618.0
15	39827.0
16	56036.0
17	53237.0
18	50438.0
19	50438.0
20	54937.5
21	59437.0
22	47833.5
23	36230.0
24	39006.0
25	41782.0
26	41782.0
27	44983.5
28	48185.0
29	50058.0
30	51931.0
31	54979.5
32	58028.0
33	58028.0
34	62535.0
35	67042.0
36	72778.5
37	78515.0
38	81087.0
39	83659.0
40	83659.0
41	88492.0
42	93325.0
43	105286.0
44	117247.0
45	144751.5
46	172256.0
47	172256.0
48	175481.0
49	178706.0
50	161068.0
51	143430.0
52	133352.0
53	123274.0
54	123274.0
55	106507.5
56	89741.0
57	75307.5
58	60874.0
59	53014.0
60	45154.0
61	45154.0
62	39263.5
63	33373.0
64	29024.0
65	24675.0
66	21149.5
67	17624.0
68	17624.0
69	14742.5
70	11861.0
71	9929.0
72	7997.0
73	6259.0
74	4521.0
75	4521.0
76	3415.0
77	2309.0
78	1817.0
79	1325.0
80	1097.5
81	870.0
82	870.0
83	695.0
84	520.0
85	403.5
86	287.0
87	257.0
88	227.0
89	227.0
90	177.0
91	127.0
92	82.5
93	38.0
94	25.5
95	13.0
96	13.0
97	6.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1802939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.95967466809232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.73745310166701	33.73353473207275
2	12.866333729205515	11.311996898139547
3	4.333991970023828	5.715626310491159
4	1.8479609518562614	3.2494304897175788
5	0.9987180445810232	2.1951660162467554
6	0.597860398500682	1.5769049169015608
7	0.3938025484240974	1.2118002338532297
8	0.2990029231705969	1.0515256981910435
9	0.24059941890477637	0.9519004962347427
>10	1.4242789924441366	12.0599923973898
>50	0.15269698826230482	4.684878642567328
>100	0.09314601762373616	7.744423102608097
>500	0.005813625941549791	1.7236199605496982
>1k	0.007077457667973658	5.661822671469159
>5k	7.582990358543205E-4	2.174025679353734
>10k+	5.05532690569547E-4	4.953351754213816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28187	1.5633917730993672	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27833	1.5437571653838538	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22455	1.245466430089981	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10682	0.592477061065294	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9042	0.5015144716487913	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6442	0.3573054884275064	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6375	0.353589333859881	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6194	0.3435501700279377	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5864	0.32524672215754385	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5214	0.2891944763522227	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4808	0.2666756889722836	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4473	0.24809491613415652	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4337	0.24055167701181238	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3610	0.20022862670339928	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3428	0.19013399787790936	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3292	0.1825907587555652	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3289	0.1824243637749253	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3278	0.18181424884591216	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2832	0.15707686172410715	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2508	0.13910620381499317	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2365	0.13117470973782253	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2241	0.12429705053803818	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2227	0.1235205406283851	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2158	0.11969345607366638	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2075	0.1150898616092946	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2018	0.11192835697713566	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1941	0.10765755247404377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.5464993546648E-5
5	0.0	0.0	0.0	0.0	5.5464993546648E-5
6	0.0	0.0	0.0	0.0	5.5464993546648E-5
7	0.0	0.0	0.0	5.5464993546648E-5	5.5464993546648E-5
8	0.0	0.0	0.0	5.5464993546648E-5	5.5464993546648E-5
9	5.5464993546648E-5	0.0	0.0	1.10929987093296E-4	5.5464993546648E-5
10	5.5464993546648E-5	0.0	0.0	1.10929987093296E-4	5.5464993546648E-5
11	5.5464993546648E-5	0.0	0.0	3.88254954826536E-4	5.5464993546648E-5
12	5.5464993546648E-5	0.0	0.0	4.43719948373184E-4	5.5464993546648E-5
13	5.5464993546648E-5	0.0	0.0	5.5464993546648E-4	5.5464993546648E-5
14	5.5464993546648E-5	0.0	0.0	6.65579922559776E-4	5.5464993546648E-5
15	5.5464993546648E-5	0.0	0.0	9.42904890293016E-4	5.5464993546648E-5
16	5.5464993546648E-5	0.0	0.0	0.001553019819306144	5.5464993546648E-5
17	5.5464993546648E-5	0.0	0.0	0.002440459716052512	5.5464993546648E-5
18	1.10929987093296E-4	0.0	0.0	0.002828714670879048	5.5464993546648E-5
19	1.10929987093296E-4	0.0	0.0	0.0032724346192522322	5.5464993546648E-5
20	1.10929987093296E-4	0.0	0.0	0.00388254954826536	5.5464993546648E-5
21	1.10929987093296E-4	0.0	0.0	0.004880919432105024	5.5464993546648E-5
22	1.10929987093296E-4	0.0	0.0	0.007154984167517592	5.5464993546648E-5
23	1.10929987093296E-4	0.0	0.0	0.011259393689969545	1.10929987093296E-4
24	1.10929987093296E-4	0.0	0.0	0.017083218012367583	1.10929987093296E-4
25	1.10929987093296E-4	0.0	0.0	0.02190867245092596	1.10929987093296E-4
26	1.10929987093296E-4	0.0	0.0	0.02828714670879048	1.10929987093296E-4
27	1.10929987093296E-4	0.0	0.0	0.07909308079752005	1.10929987093296E-4
28	1.10929987093296E-4	0.0	0.0	0.13627748914411414	1.10929987093296E-4
29	1.10929987093296E-4	0.0	0.0	0.20017316170985264	1.10929987093296E-4
30	1.10929987093296E-4	0.0	0.0	0.32496939718981066	1.66394980639944E-4
31	1.10929987093296E-4	0.0	0.0	0.4930837926297007	1.66394980639944E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTG	25	0.0054971618	29.6	30
GGTATCA	6435	0.0	27.915308	1
GTATTGG	515	0.0	21.553396	1
ATTGGAC	560	0.0	19.821428	3
AGTACCG	125	4.129106E-10	19.24	5
ACGTTTA	100	2.8771683E-7	18.5	26
CGCATAC	60	9.2395936E-4	18.5	37
CGTCTGT	455	0.0	18.296703	34
TCACGTT	105	4.801004E-7	17.619047	24
GCGCTTA	65	0.0015804048	17.076923	18
GTATCAA	10695	0.0	16.848059	2
TACACCG	110	7.813669E-7	16.818182	5
GGACCCT	905	0.0	16.76243	6
ACGGACC	145	2.9831426E-9	16.586208	8
ATCACGT	115	1.2430773E-6	16.086956	23
TCTACGC	70	0.0025932607	15.857142	2
TATTGGA	680	0.0	15.779412	2
CGACGAG	95	7.063236E-5	15.578948	24
TGCGACG	85	5.366252E-4	15.235294	22
CGGACCA	160	1.0975782E-8	15.03125	9
>>END_MODULE