FastQCFastQC Report
Wed 25 May 2016
SRR2088358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172665
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235712.0100369670792597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225101.9195592944276498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142881.2184212882622063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75930.6474994990044045No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49300.42040992099192864No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT48480.41341730161640367No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT47810.4077038199315235No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA39720.33871566048274654No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA38020.3242187666554387No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37210.31731142312595667No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30510.260176606277155No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG30480.2599207787390261No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT27470.23425274908008678No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25780.21984113109882192No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT25680.21898837263839205No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.17839706992192997No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.14837997211479834No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT17180.14650390350185263No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA16840.14360452473639104No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC16510.14079042181697243No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16380.13968183581841362No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15650.13345669905727553No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14670.12509966614506274No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14440.12313832168607403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC14260.12160335645730025No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13730.1170837366170219No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA13700.11682790907889294No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.11137025493214174No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA12880.10983528970336796No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12270.10463346309474574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54450.026.7052351
GTCGCAA358.8695006E-426.42857219
TTGCGTG358.8695006E-426.4285729
CGCGATA659.39217E-825.61538514
GCGATAT753.7408972E-722.215
ACGTGCG806.960363E-720.81259
TGCGCGA909.478754E-820.55555512
TGCACGG450.003825853320.5555555
CGTCTGT2450.019.63265234
CGATATG1002.87504E-718.516
GTATCAA80600.018.086852
TACTACG953.606514E-617.5263165
ATACCGT1900.017.5263166
ACGTTTA752.0673111E-417.26666626
TATAGTG1301.393164E-817.0769235
TGCGTGT650.001579960117.07692310
ACGATCT1107.807939E-716.81818232
ATTGGAC4200.016.2976193
GGCTACT1251.6576814E-716.2799992
GTATTGG4800.016.18751
Produced by FastQC (version 0.11.5)