##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1172665
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.682129167324	31.0	30.0	31.0	28.0	34.0
2	30.866719821944034	31.0	31.0	33.0	27.0	34.0
3	30.844958278792323	31.0	30.0	34.0	27.0	34.0
4	34.68863912541092	35.0	35.0	37.0	32.0	37.0
5	34.4812585009359	35.0	35.0	37.0	30.0	37.0
6	34.66106432783446	35.0	35.0	37.0	31.0	37.0
7	34.49071388674515	35.0	35.0	37.0	30.0	37.0
8	34.6223866151032	35.0	35.0	37.0	30.0	37.0
9	35.95168611666588	37.0	35.0	39.0	30.0	39.0
10	35.73772987170249	37.0	35.0	39.0	30.0	39.0
11	35.98193516477426	37.0	35.0	39.0	30.0	39.0
12	35.73892543906401	37.0	35.0	39.0	30.0	39.0
13	35.86280566061066	37.0	35.0	39.0	30.0	39.0
14	36.603561119330756	38.0	35.0	40.0	30.0	41.0
15	36.65618569668234	38.0	35.0	40.0	31.0	41.0
16	36.522839003466466	38.0	35.0	40.0	30.0	41.0
17	36.656423616292805	38.0	36.0	40.0	31.0	41.0
18	36.64859699914298	38.0	36.0	40.0	30.0	41.0
19	36.750138360060205	39.0	36.0	40.0	30.0	41.0
20	36.73506073772134	39.0	35.0	40.0	30.0	41.0
21	36.71441033884357	38.0	35.0	40.0	30.0	41.0
22	36.57780184451655	38.0	35.0	40.0	30.0	41.0
23	36.43108389864113	38.0	35.0	40.0	30.0	41.0
24	36.33952748653707	38.0	35.0	40.0	30.0	41.0
25	36.25281985903903	38.0	35.0	40.0	30.0	41.0
26	35.96060255912814	38.0	34.0	40.0	29.0	41.0
27	35.740817710087704	38.0	34.0	40.0	28.0	41.0
28	35.580001108585996	38.0	34.0	40.0	27.0	41.0
29	35.385166266580825	38.0	34.0	40.0	27.0	41.0
30	35.13598683340937	37.0	33.0	40.0	27.0	41.0
31	34.81796762076126	37.0	33.0	40.0	26.0	41.0
32	34.65125931105644	37.0	33.0	40.0	25.0	41.0
33	34.66516524327067	37.0	33.0	40.0	25.0	41.0
34	34.57118614438053	37.0	33.0	40.0	25.0	41.0
35	34.41660576549995	37.0	33.0	40.0	25.0	41.0
36	34.258025949439954	37.0	33.0	40.0	24.0	41.0
37	34.06285938439367	37.0	33.0	40.0	23.0	41.0
38	33.90645580792469	37.0	33.0	40.0	23.0	41.0
39	33.7169592338818	37.0	33.0	40.0	21.0	41.0
40	33.461939258014866	37.0	32.0	40.0	20.0	41.0
41	33.23170555955878	37.0	32.0	40.0	18.0	41.0
42	32.9667884690001	36.0	31.0	40.0	16.0	41.0
43	32.55079242579936	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	11.0
15	17.0
16	38.0
17	99.0
18	220.0
19	462.0
20	947.0
21	1862.0
22	3105.0
23	4959.0
24	7576.0
25	11405.0
26	16341.0
27	22493.0
28	29746.0
29	38276.0
30	47187.0
31	55873.0
32	66212.0
33	78024.0
34	91908.0
35	110724.0
36	139146.0
37	175683.0
38	184219.0
39	86127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.48817437204999	25.426869566329685	15.630124545373144	19.454831516247182
2	21.20315691182051	25.73778530100242	31.84549722213931	21.213560565037756
3	24.13101780986045	25.613708945009872	31.75919806594381	18.496075179185873
4	16.31275769294726	18.174585239603807	32.350671334097974	33.16198573335096
5	11.264939262278656	42.46651857094737	33.11568094894962	13.152861217824357
6	32.21098949827956	36.22390026137047	14.110508968887109	17.454601271462863
7	22.851453739985416	36.69624317260258	21.599945423458532	18.852357663953473
8	28.33648143331642	34.167558509889865	19.997527000464753	17.49843305632896
9	24.817232542968366	13.151667355979754	24.423343410095807	37.607756690956066
10	17.201673112099364	29.298307700835274	32.741405260666944	20.758613926398418
11	31.868521700570923	21.82481782947389	24.132467499243177	22.17419297071201
12	19.210345665641935	29.285601599774875	31.78904461205886	19.715008122524335
13	31.555815173131286	20.52035321255431	29.737648859648747	18.186182754665655
14	20.5302452106953	22.983631301352048	30.095807413029295	26.39031607492336
15	23.669419655229753	29.21678399201818	27.308140005884034	19.80565634686803
16	21.095794621652388	27.39631523069248	29.233071678612394	22.27481846904274
17	18.890134863750514	27.464280079988747	29.97463043580221	23.670954620458527
18	19.68737874840641	24.8752201182776	34.543454439247355	20.89394669406864
19	19.252642485279257	24.39545820843975	36.50428724315981	19.847612063121183
20	22.97450678582545	23.378714296069212	35.15582028968205	18.490958628423293
21	23.048782047728892	23.623711801750712	33.84768881138262	19.479817339137774
22	21.199319498748576	23.832296521171862	34.3878260202189	20.58055795986066
23	20.152302661032774	26.07411323779596	35.261647614621396	18.511936486549867
24	18.993062809924403	25.15441323822234	34.169519854348856	21.6830040975044
25	19.83644092728955	26.023203557708296	34.781374049707296	19.358981465294864
26	21.034054909117266	24.84759074415967	33.896040216089	20.22231413063407
27	20.559835929272214	25.843100970865507	33.57813186204074	20.018931237821544
28	19.944400148379973	26.279542750913514	33.68097453236858	20.095082568337933
29	18.965518711652518	25.62198070207604	34.50030486114961	20.912195725121837
30	18.402698127768797	26.185739320266233	35.9912677533652	19.420294798599773
31	19.67919226718628	25.27490800868108	34.82767883410863	20.218220890024003
32	20.203212341120437	26.409076761052813	33.0751749220792	20.31253597574755
33	21.16947295263353	26.197592662866203	32.425799354461844	20.20713503003842
34	20.129704561831385	28.36845987558254	32.56360512166731	18.938230440918762
35	18.774841919900396	29.052457436693345	32.69646488980229	19.47623575360397
36	19.710147399299885	25.94824608903651	34.4905834147007	19.8510230969629
37	19.48356947636367	26.441822685933325	33.694107012659195	20.38050082504381
38	20.289511497315942	25.981674220685363	33.51613632196748	20.21267796003121
39	21.146960129278185	25.803191874917392	33.20385617375806	19.845991822046365
40	19.634763551397885	26.61186272294304	32.483360550540866	21.270013175118212
41	19.90866956888796	26.428178550566443	32.27639607219453	21.38675580835106
42	18.267621187636706	27.55944792417272	32.33907381903613	21.833857069154448
43	18.791129606494607	27.278634563153158	31.945184686163568	21.985051144188663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	152.5
2	183.0
3	458.0
4	733.0
5	733.0
6	1642.0
7	2551.0
8	2944.5
9	3338.0
10	5105.0
11	6872.0
12	6872.0
13	13251.0
14	19630.0
15	33083.0
16	46536.0
17	41679.5
18	36823.0
19	36823.0
20	41612.0
21	46401.0
22	35794.5
23	25188.0
24	26076.5
25	26965.0
26	26965.0
27	28433.0
28	29901.0
29	31061.0
30	32221.0
31	33622.5
32	35024.0
33	35024.0
34	37273.0
35	39522.0
36	42764.0
37	46006.0
38	47756.0
39	49506.0
40	49506.0
41	52553.5
42	55601.0
43	63542.5
44	71484.0
45	88598.5
46	105713.0
47	105713.0
48	110214.0
49	114715.0
50	103848.0
51	92981.0
52	86402.0
53	79823.0
54	79823.0
55	68645.5
56	57468.0
57	48948.0
58	40428.0
59	35361.0
60	30294.0
61	30294.0
62	26889.5
63	23485.0
64	20387.5
65	17290.0
66	14893.5
67	12497.0
68	12497.0
69	10626.0
70	8755.0
71	7389.0
72	6023.0
73	4948.0
74	3873.0
75	3873.0
76	2976.5
77	2080.0
78	1619.0
79	1158.0
80	935.5
81	713.0
82	713.0
83	513.0
84	313.0
85	260.5
86	208.0
87	166.0
88	124.0
89	124.0
90	105.5
91	87.0
92	52.0
93	17.0
94	13.0
95	9.0
96	9.0
97	6.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1172665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.85095173809155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20702496579739	40.482474823639606
2	10.783113753087795	10.750969665830617
3	3.2523718634000707	4.864014983900514
4	1.4102399834411006	2.8120722141459735
5	0.7724757682522273	1.9254326120993488
6	0.47179046962537624	1.4111522359071709
7	0.33571608462792984	1.1715036432741595
8	0.2285757288740494	0.911577410287947
9	0.17703280285229972	0.7942728339944161
>10	1.1770667520102824	11.150763668537618
>50	0.11244522377632149	3.8276414437598225
>100	0.059636623955631615	5.695234886617455
>500	0.005826566166914158	2.1863085182241075
>1k	0.0059979357600586924	6.210630000249077
>5k	1.7136959314453405E-4	0.6486652304968602
>10k+	5.141087794336022E-4	5.15728582903529
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23571	2.0100369670792597	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22510	1.9195592944276498	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14288	1.2184212882622063	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7593	0.6474994990044045	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4930	0.42040992099192864	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4848	0.41341730161640367	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4781	0.4077038199315235	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3972	0.33871566048274654	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3802	0.3242187666554387	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3721	0.31731142312595667	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3051	0.260176606277155	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3048	0.2599207787390261	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2747	0.23425274908008678	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2578	0.21984113109882192	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2568	0.21898837263839205	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2092	0.17839706992192997	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1740	0.14837997211479834	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1718	0.14650390350185263	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1684	0.14360452473639104	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1651	0.14079042181697243	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1638	0.13968183581841362	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1565	0.13345669905727553	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1467	0.12509966614506274	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1444	0.12313832168607403	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1426	0.12160335645730025	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1373	0.1170837366170219	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1370	0.11682790907889294	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1306	0.11137025493214174	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1288	0.10983528970336796	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1227	0.10463346309474574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.527584604298755E-5	0.0	0.0	0.0	0.0
9	8.527584604298755E-5	0.0	0.0	0.0	0.0
10	8.527584604298755E-5	0.0	0.0	0.0	0.0
11	8.527584604298755E-5	0.0	0.0	8.527584604298755E-5	0.0
12	8.527584604298755E-5	0.0	0.0	1.705516920859751E-4	0.0
13	8.527584604298755E-5	0.0	0.0	1.705516920859751E-4	0.0
14	8.527584604298755E-5	0.0	0.0	3.411033841719502E-4	0.0
15	8.527584604298755E-5	0.0	0.0	5.116550762579253E-4	0.0
16	8.527584604298755E-5	0.0	0.0	0.0011938618446018258	0.0
17	8.527584604298755E-5	0.0	0.0	0.002302447843160664	0.0
18	8.527584604298755E-5	0.0	0.0	0.002472999535246639	0.0
19	8.527584604298755E-5	0.0	0.0	0.0031552063035905397	0.0
20	8.527584604298755E-5	0.0	0.0	0.0037521372258914523	0.0
21	8.527584604298755E-5	0.0	0.0	0.005116550762579253	0.0
22	8.527584604298755E-5	0.0	0.0	0.008101205374083818	0.0
23	8.527584604298755E-5	0.0	0.0	0.012450273522276184	0.0
24	8.527584604298755E-5	8.527584604298755E-5	0.0	0.020807306434488963	0.0
25	8.527584604298755E-5	8.527584604298755E-5	0.0	0.025326926274767302	0.0
26	8.527584604298755E-5	8.527584604298755E-5	0.0	0.03325757995676515	0.0
27	8.527584604298755E-5	8.527584604298755E-5	0.0	0.09030712095952383	0.0
28	8.527584604298755E-5	8.527584604298755E-5	0.0	0.1412168010471874	0.0
29	8.527584604298755E-5	8.527584604298755E-5	0.0	0.2040650995808692	0.0
30	8.527584604298755E-5	8.527584604298755E-5	0.0	0.31688504389574174	0.0
31	8.527584604298755E-5	8.527584604298755E-5	0.0	0.4754981175356986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5445	0.0	26.705235	1
GTCGCAA	35	8.8695006E-4	26.428572	19
TTGCGTG	35	8.8695006E-4	26.428572	9
CGCGATA	65	9.39217E-8	25.615385	14
GCGATAT	75	3.7408972E-7	22.2	15
ACGTGCG	80	6.960363E-7	20.8125	9
TGCGCGA	90	9.478754E-8	20.555555	12
TGCACGG	45	0.0038258533	20.555555	5
CGTCTGT	245	0.0	19.632652	34
CGATATG	100	2.87504E-7	18.5	16
GTATCAA	8060	0.0	18.08685	2
TACTACG	95	3.606514E-6	17.526316	5
ATACCGT	190	0.0	17.526316	6
ACGTTTA	75	2.0673111E-4	17.266666	26
TATAGTG	130	1.393164E-8	17.076923	5
TGCGTGT	65	0.0015799601	17.076923	10
ACGATCT	110	7.807939E-7	16.818182	32
ATTGGAC	420	0.0	16.297619	3
GGCTACT	125	1.6576814E-7	16.279999	2
GTATTGG	480	0.0	16.1875	1
>>END_MODULE