FastQCFastQC Report
Wed 25 May 2016
SRR2088357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941948
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231741.1933378236698409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT228211.1751601999641597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181470.9344740435892207No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG79270.4081983657646858No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77880.40104060458879437No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT51580.2656095837787623No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT50140.25819434917927775No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50020.257576412962654No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46940.24171605006931182No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA39830.20510332923435642No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG35120.18084933273187542No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32730.16854210308411965No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32500.16735772533559087No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28920.14892262820631655No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26510.1365124091891235No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26260.13522504207115743No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25430.13095098323951002No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22680.11678994494188309No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA22520.1159660299863848No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22060.11359727448932722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50800.029.0246071
GTCGTAC358.871606E-426.4285721
ATCACGT1202.382876E-1020.04166823
CGACTAT656.905265E-519.92307736
GTATTGG5100.019.5882361
ACGTTTA1254.129106E-1019.24000226
ATTGGAC5050.018.6831683
TCACGTT1451.546141E-1017.86206824
GTATCAA85150.017.4245432
TAAACGT1603.45608E-1117.343754
ACGTGAT1401.87174E-917.1785727
GCGTTAT904.448498E-516.4444451
AACGATA904.448498E-516.4444454
TGTACCG700.002593356415.8571435
TATACTG2100.015.8571425
ATAGTAC1455.351103E-815.3103453
GTCGACG855.3665164E-415.23529436
CAATTCG1252.9611037E-614.80000115
GACGTTA1001.0939806E-414.87
ACGATAG1001.0939806E-414.85
Produced by FastQC (version 0.11.5)