FastQCFastQC Report
Wed 25 May 2016
SRR2088356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458448
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82291.7949691131818657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78581.7140439046522178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45530.9931333542735491No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.5723658953687223No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT15650.3413691411021534No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT14840.3237008341185914No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG14290.31170383554950615No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA11360.24779255226328833No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA11300.2464837887830245No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.2371043171744669No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9640.21027466582905804No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG9290.2026402121941856No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT9070.19784141276655148No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.17602868809548738No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7410.16163228981258507No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT6540.1426552193487593No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA6440.14047394688165288No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT5890.12847694831256762No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.12825882106585698No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA5200.11342616828953338No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG4920.10731860538163542No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC4810.10491920566781839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4740.10339231494084389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA250.00549293929.622
GGTATCA18850.028.7559681
TTAGAGT509.0755275E-625.9000024
GTATCAA26400.020.5321962
TAGAGTG656.890569E-519.9230755
AGTACTG1457.2759576E-1219.137935
GGTAATA701.2171697E-418.52
CGTCTGT801.6132175E-518.534
TTATCTA500.007028588618.54
ATACTCT500.007028588618.54
TGTGAAC500.007028588618.53
GTACTGT500.007028588618.56
CCGGTCG500.007028588618.528
ATACGGA500.007028588618.529
TAGTAGT500.007028588618.54
TGCGTCT801.6132175E-518.532
TAGAGTC650.001577976317.0769215
ATTAGAG650.001577976317.0769213
ACACCGC650.001577976317.0769216
CCGTGAA650.001577976317.0769219
Produced by FastQC (version 0.11.5)