##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458448
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.645349090845635	31.0	30.0	31.0	27.0	34.0
2	30.841118294768435	31.0	31.0	33.0	27.0	34.0
3	30.807899696366874	31.0	30.0	34.0	27.0	34.0
4	34.643508096883394	35.0	35.0	37.0	32.0	37.0
5	34.38689229749066	35.0	35.0	37.0	30.0	37.0
6	34.60352319128887	35.0	35.0	37.0	31.0	37.0
7	34.45361087844205	35.0	35.0	37.0	30.0	37.0
8	34.574226520783164	35.0	35.0	37.0	30.0	37.0
9	35.93341883921404	37.0	35.0	39.0	30.0	39.0
10	35.680570097372005	37.0	35.0	39.0	30.0	39.0
11	35.94325201549576	37.0	35.0	39.0	30.0	39.0
12	35.689295187240425	37.0	35.0	39.0	30.0	39.0
13	35.830896851987575	37.0	35.0	39.0	30.0	39.0
14	36.589938226363735	38.0	35.0	40.0	30.0	41.0
15	36.64031689526402	38.0	35.0	40.0	30.0	41.0
16	36.50282692911737	38.0	35.0	40.0	30.0	41.0
17	36.60404451540851	38.0	35.0	40.0	30.0	41.0
18	36.59698155516002	38.0	35.0	40.0	30.0	41.0
19	36.69652174292395	38.0	35.0	40.0	30.0	41.0
20	36.669360538163545	38.0	35.0	40.0	30.0	41.0
21	36.654141800160545	38.0	35.0	40.0	30.0	41.0
22	36.515013698391094	38.0	35.0	40.0	30.0	41.0
23	36.37844422922556	38.0	35.0	40.0	30.0	41.0
24	36.27760182179876	38.0	35.0	40.0	30.0	41.0
25	36.186522789934735	38.0	34.0	40.0	30.0	41.0
26	35.8997945241336	38.0	34.0	40.0	29.0	41.0
27	35.68631775032283	38.0	34.0	40.0	27.0	41.0
28	35.532564216661434	38.0	34.0	40.0	27.0	41.0
29	35.349636163752486	38.0	34.0	40.0	27.0	41.0
30	35.09371837154923	37.0	33.0	40.0	27.0	41.0
31	34.804291871706276	37.0	33.0	40.0	26.0	41.0
32	34.663870275363834	37.0	33.0	40.0	25.0	41.0
33	34.709306180853666	37.0	33.0	40.0	25.0	41.0
34	34.64066589885876	37.0	33.0	40.0	25.0	41.0
35	34.49241571563187	37.0	33.0	40.0	25.0	41.0
36	34.3526528635745	37.0	33.0	40.0	24.0	41.0
37	34.196303615677245	37.0	33.0	40.0	24.0	41.0
38	34.04948216591631	37.0	33.0	40.0	23.0	41.0
39	33.884348933794016	37.0	33.0	40.0	22.0	41.0
40	33.65496413988064	37.0	32.0	40.0	21.0	41.0
41	33.454771315394545	37.0	32.0	40.0	19.0	41.0
42	33.21785022510732	37.0	31.0	40.0	18.0	41.0
43	32.811018915994836	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	3.0
15	4.0
16	12.0
17	36.0
18	82.0
19	165.0
20	378.0
21	649.0
22	1143.0
23	1957.0
24	3076.0
25	4464.0
26	6227.0
27	8642.0
28	11494.0
29	14792.0
30	18329.0
31	21670.0
32	25744.0
33	30874.0
34	36442.0
35	43776.0
36	55353.0
37	68693.0
38	71538.0
39	32901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71693190939867	25.927913307507065	15.659573168603636	19.695581614490628
2	21.79462010958713	25.959105503786688	31.22295222140788	21.023322165218303
3	24.018863644295536	25.447815237496947	31.656371060621925	18.876950057585592
4	17.225508672739327	18.965727846996824	32.415672006421666	31.393091473842183
5	11.61593026908177	42.360747565699924	33.217944019823406	12.805378145394897
6	31.914415593480612	37.273147663420936	14.25919624472132	16.553240498377132
7	23.02420339929501	36.57797612815412	22.038486371409626	18.359334101141243
8	27.543145569399364	34.91999092590653	20.167827103619167	17.36903640107493
9	24.721887760443934	13.627935992740724	24.038495096499492	37.61168115031585
10	16.969209157854326	29.599867378634	33.121749904024014	20.30917355948766
11	32.270399260112384	22.50636931560395	23.742060168219734	21.481171256063938
12	18.706810805151296	29.653308554078105	31.775904791819354	19.86397584895124
13	30.787570236973437	21.29096429693226	29.248028129689736	18.673437336404564
14	20.224540187763935	23.566467734617667	29.962176735420375	26.246815342198026
15	23.46285729243011	30.153910585279025	26.492644749240917	19.890587373049943
16	21.132603915820333	28.12118277318256	28.87895682825533	21.867256482741773
17	19.081335287753465	28.40562070289324	29.78462115659791	22.728422852755383
18	19.63843227585244	25.841971172303076	33.50652636722158	21.0130701846229
19	19.545510068753707	25.52634104631278	35.38111192545283	19.547036959480682
20	22.327504973301224	24.75198932049	34.40368373294245	18.516821973266325
21	22.384436184692703	24.910349701601927	33.18369804208983	19.521516071615537
22	21.11035493665585	25.15945101734548	33.66401423934667	20.06617980665201
23	20.263148710431718	26.849282797612815	34.20017101176142	18.687397480194047
24	19.194543328796286	26.141023627543365	33.36692492932677	21.297508114333578
25	19.902366244372317	26.729967193662098	33.954995986458655	19.412670575506926
26	20.678026733675356	26.057044637559766	33.07049000104701	20.194438627717865
27	20.65817715422469	26.79889540362266	32.79063274351726	19.752294698635396
28	20.107842110773742	27.135247618050467	33.02773706069173	19.72917321048407
29	19.248638885980526	26.505950511290266	33.707857815935505	20.537552786793704
30	18.908142253865215	26.977541618678675	34.77580881583081	19.33850731162531
31	19.82776672599728	26.298947754161865	33.81910271175793	20.054182808082924
32	20.062035388964507	27.196105119882734	32.58864691306321	20.153212578089555
33	20.872378110494537	27.04865110110634	31.960658569783266	20.118312218615852
34	20.265329982898823	28.34061878337347	32.217612466408404	19.176438767319304
35	19.122561337381775	28.816790562942796	32.40781942554008	19.652828674135346
36	19.809007782780164	26.568989285589637	33.6912801451855	19.9307227864447
37	19.65762747356298	26.829215090915437	33.18413429658326	20.329023138938332
38	20.146232506194814	26.475412696750777	33.118914249816775	20.259440547237638
39	20.84969287683663	26.3303580776882	32.908639583987714	19.911309461487452
40	19.68249397968799	26.767921334589744	32.41087320699403	21.13871147872823
41	19.97151258157959	26.440076082783655	32.520809339336196	21.06760199630056
42	18.439823055177467	27.736406309984996	32.03198617945765	21.79178445537989
43	18.853828569434263	27.235149897043943	31.98748822112868	21.92353331239312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	88.5
2	83.0
3	181.0
4	279.0
5	279.0
6	570.0
7	861.0
8	1030.5
9	1200.0
10	1816.0
11	2432.0
12	2432.0
13	4682.0
14	6932.0
15	11626.5
16	16321.0
17	14542.0
18	12763.0
19	12763.0
20	15056.0
21	17349.0
22	13672.0
23	9995.0
24	10756.5
25	11518.0
26	11518.0
27	12110.5
28	12703.0
29	13133.0
30	13563.0
31	14481.5
32	15400.0
33	15400.0
34	16589.0
35	17778.0
36	19189.5
37	20601.0
38	21611.0
39	22621.0
40	22621.0
41	23784.0
42	24947.0
43	27357.0
44	29767.0
45	34857.0
46	39947.0
47	39947.0
48	40812.5
49	41678.0
50	37518.5
51	33359.0
52	30984.0
53	28609.0
54	28609.0
55	24758.0
56	20907.0
57	18303.0
58	15699.0
59	13868.0
60	12037.0
61	12037.0
62	10587.5
63	9138.0
64	7984.5
65	6831.0
66	5794.5
67	4758.0
68	4758.0
69	3974.5
70	3191.0
71	2713.0
72	2235.0
73	1758.5
74	1282.0
75	1282.0
76	984.5
77	687.0
78	511.5
79	336.0
80	301.5
81	267.0
82	267.0
83	194.5
84	122.0
85	97.5
86	73.0
87	57.5
88	42.0
89	42.0
90	35.0
91	28.0
92	18.0
93	8.0
94	7.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	458448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.74003716044977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.35105921025156	50.13524389693925
2	8.5702322407547	10.068315205912318
3	2.365582658625645	4.168632398213578
4	1.050743183679868	2.4688277462177903
5	0.5837101982742987	1.7143579368782902
6	0.3913538623555894	1.379288425059172
7	0.2560196760180099	1.052702369817295
8	0.19699622890393265	0.9257252645028379
9	0.1549250327794203	0.8190271964292343
>10	0.9581052936765094	10.67966122943324
>50	0.06828569811813699	2.8018550884088573
>100	0.045523798736632565	5.361290421061437
>500	0.0037314589128387347	1.6094830564944453
>1k	0.002985167130270988	3.2895440163242045
>5k	7.46291782567747E-4	3.5260457483080674
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8229	1.7949691131818657	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7858	1.7140439046522178	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4553	0.9931333542735491	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2624	0.5723658953687223	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1565	0.3413691411021534	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1484	0.3237008341185914	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1429	0.31170383554950615	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1136	0.24779255226328833	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1130	0.2464837887830245	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.2371043171744669	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	964	0.21027466582905804	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	929	0.2026402121941856	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	907	0.19784141276655148	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	807	0.17602868809548738	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	741	0.16163228981258507	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	654	0.1426552193487593	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	644	0.14047394688165288	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	589	0.12847694831256762	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	588	0.12825882106585698	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	520	0.11342616828953338	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	492	0.10731860538163542	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	481	0.10491920566781839	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	474	0.10339231494084389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	4.3625449342128225E-4	0.0
16	0.0	0.0	0.0	8.725089868425645E-4	0.0
17	0.0	0.0	0.0	0.001526890726974488	0.0
18	0.0	0.0	0.0	0.001745017973685129	0.0
19	0.0	0.0	0.0	0.0023993997138170523	0.0
20	0.0	0.0	0.0	0.003053781453948976	0.0
21	0.0	0.0	0.0	0.0050169266743447455	0.0
22	0.0	0.0	0.0	0.008725089868425644	0.0
23	0.0	0.0	0.0	0.012433253062506543	0.0
24	0.0	0.0	0.0	0.020067706697378982	0.0
25	0.0	0.0	0.0	0.02508463337172373	0.0
26	0.0	0.0	0.0	0.032064705266464245	0.0
27	0.0	0.0	0.0	0.08354273549017555	0.0
28	0.0	0.0	0.0	0.13720203818099327	0.0
29	0.0	0.0	0.0	0.19435137681918124	0.0
30	0.0	0.0	0.0	0.30777754510871463	0.0
31	0.0	0.0	0.0	0.4971119952535511	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGGA	25	0.005492939	29.6	22
GGTATCA	1885	0.0	28.755968	1
TTAGAGT	50	9.0755275E-6	25.900002	4
GTATCAA	2640	0.0	20.532196	2
TAGAGTG	65	6.890569E-5	19.923075	5
AGTACTG	145	7.2759576E-12	19.13793	5
GGTAATA	70	1.2171697E-4	18.5	2
CGTCTGT	80	1.6132175E-5	18.5	34
TTATCTA	50	0.0070285886	18.5	4
ATACTCT	50	0.0070285886	18.5	4
TGTGAAC	50	0.0070285886	18.5	3
GTACTGT	50	0.0070285886	18.5	6
CCGGTCG	50	0.0070285886	18.5	28
ATACGGA	50	0.0070285886	18.5	29
TAGTAGT	50	0.0070285886	18.5	4
TGCGTCT	80	1.6132175E-5	18.5	32
TAGAGTC	65	0.0015779763	17.076921	5
ATTAGAG	65	0.0015779763	17.076921	3
ACACCGC	65	0.0015779763	17.076921	6
CCGTGAA	65	0.0015779763	17.076921	9
>>END_MODULE