FastQCFastQC Report
Wed 25 May 2016
SRR2088355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797472
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60060.7531298904538342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58220.73005698005698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50820.6372637534609366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.28352092612655994No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG13910.1744261867501304No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG13390.167905581637976No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA12640.15850086272621483No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12620.1582500702219012No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA12610.1581246739697444No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9540.11962802455760203No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA8540.10708839934192046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.029.4067171
TCACGTT802.723209E-823.12500224
TAGGTTG705.098689E-621.1428595
CTAATAC1351.8189894E-1220.5555533
CGGTATA555.1411963E-420.18181826
TACCCCG609.2334347E-418.55
ACGTTTA902.1505348E-618.526
GTTTACC801.6156586E-518.515
TATACTG1700.018.55
AGTTCCG500.0070330418.4999985
TTGGACC2450.018.1224484
TGGACTG1502.5102054E-1017.2666665
CTAGACA650.001579360317.0769234
CACGTTT1107.800227E-716.81818225
TCTTACC1554.0017767E-1016.7096772
GTATTGA803.3809402E-416.18751
GATATAC803.3809402E-416.18751
GTATCAA24600.016.0934962
TAGACAG1151.2409528E-616.0869565
CGGTGTG700.002591560115.85714330
Produced by FastQC (version 0.11.5)