FastQCFastQC Report
Wed 25 May 2016
SRR2088354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426512
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234970.9683446857052427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT226890.9350458600658064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195820.8070019847418847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86480.35639634174485846No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA70940.2923537983739623No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG69390.2859660286040209No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG61830.25481019669385524No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA60930.2511011690855022No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA60140.2478454670737256No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG56690.23362752790837218No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37780.15569673671508732No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA34460.1420145459820516No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31610.13026929188893357No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.12631299577335697No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC30470.12557119025168637No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28150.1160101413057096No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA26310.10842724041752111No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG25000.10302854467647389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.029.3612881
GTATTGG7300.019.006851
GTATCAA106150.017.1493172
ATTGGAC7600.016.5526313
CGAACTA2100.015.85714424
GCGAACT2450.015.85714223
CGAATTA1659.749783E-1015.6969715
TATACTG3850.015.3766235
GGACCCT12200.014.8606566
TTGGACC12700.014.8582674
AGCGAAC2500.014.79999922
ACCGTCG1051.6568275E-414.09523923
GTATTAG3050.013.950821
TGGACCC13700.013.6386865
TATACCG1501.3070949E-613.5666675
CGTATAC1652.59839E-713.4545453
GTCTATA1652.59839E-713.4545451
TTGATAC20900.013.36602934
GAACCGT850.00940866513.0588256
ACACCGC1857.3932824E-813.06
Produced by FastQC (version 0.11.5)