##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2426512
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.767163319200563	31.0	31.0	33.0	28.0	34.0
2	31.09216150589818	31.0	31.0	34.0	28.0	34.0
3	31.1673744040829	31.0	31.0	34.0	28.0	34.0
4	35.06883172224164	35.0	35.0	37.0	32.0	37.0
5	34.75124870596148	35.0	35.0	37.0	32.0	37.0
6	34.83457572021074	35.0	35.0	37.0	32.0	37.0
7	34.65386901033253	35.0	35.0	37.0	30.0	37.0
8	34.714245385969654	35.0	35.0	37.0	31.0	37.0
9	36.185443962362434	38.0	35.0	39.0	32.0	39.0
10	35.93886657061659	37.0	35.0	39.0	30.0	39.0
11	36.06699039609118	37.0	35.0	39.0	31.0	39.0
12	35.81911731736748	37.0	35.0	39.0	30.0	39.0
13	35.90542515347132	37.0	35.0	39.0	30.0	39.0
14	36.673993369907095	38.0	36.0	40.0	30.0	41.0
15	36.8096918539863	38.0	36.0	40.0	31.0	41.0
16	36.790219046928264	38.0	36.0	40.0	31.0	41.0
17	36.850105006692736	38.0	36.0	40.0	31.0	41.0
18	36.844978306309635	38.0	36.0	40.0	31.0	41.0
19	36.93321195197056	39.0	36.0	40.0	31.0	41.0
20	36.917559443349134	39.0	36.0	40.0	31.0	41.0
21	36.82522155258247	39.0	36.0	40.0	30.0	41.0
22	36.75114196838919	39.0	35.0	40.0	30.0	41.0
23	36.64218557336621	38.0	35.0	40.0	30.0	41.0
24	36.56697926900836	38.0	35.0	40.0	30.0	41.0
25	36.44356055111205	38.0	35.0	40.0	30.0	41.0
26	36.19515831778289	38.0	35.0	40.0	30.0	41.0
27	36.061007734558906	38.0	34.0	40.0	29.0	41.0
28	35.91423079712773	38.0	34.0	40.0	29.0	41.0
29	35.698979028333675	38.0	34.0	40.0	27.0	41.0
30	35.49128254877783	38.0	34.0	40.0	27.0	41.0
31	35.295016055968404	38.0	34.0	40.0	27.0	41.0
32	35.17894327330753	38.0	33.0	40.0	27.0	41.0
33	35.01913528554567	37.0	33.0	40.0	26.0	41.0
34	35.204434183717204	38.0	33.0	40.0	27.0	41.0
35	35.14255853669794	38.0	33.0	40.0	26.0	41.0
36	35.09655711572825	38.0	33.0	40.0	26.0	41.0
37	34.99525574157474	38.0	33.0	40.0	25.0	41.0
38	34.98579442426001	38.0	33.0	40.0	25.0	41.0
39	34.91122112728064	38.0	33.0	40.0	25.0	41.0
40	34.75898614966668	38.0	33.0	40.0	24.0	41.0
41	34.65847521050792	38.0	33.0	40.0	24.0	41.0
42	34.53547190370375	38.0	33.0	40.0	24.0	41.0
43	34.174330479305276	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	4.0
15	14.0
16	30.0
17	98.0
18	239.0
19	534.0
20	1099.0
21	2242.0
22	4049.0
23	6903.0
24	11439.0
25	18193.0
26	26894.0
27	38872.0
28	53322.0
29	70575.0
30	89055.0
31	108374.0
32	131334.0
33	155673.0
34	186016.0
35	218722.0
36	263797.0
37	325145.0
38	393611.0
39	320273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9740450490251	22.69896048319563	13.894058632308434	23.432935835470833
2	18.56673282472949	23.960812886975212	35.22603638473661	22.246417903558687
3	19.744884838813903	25.676815115688694	32.55825646030186	22.02004358519554
4	13.861748880697892	16.824561345668183	36.89979691013273	32.41389286350119
5	12.304740302129147	38.649386444410744	35.71843040545441	13.327442848005697
6	31.671963707576968	37.90403674080326	14.257090012330456	16.166909539289318
7	25.029260106688117	33.63585261478204	22.537535359396532	18.797351919133305
8	25.900016154875804	34.979427260199	19.98819704992186	19.132359535003328
9	24.321618850432223	14.537822190864913	22.621647863270404	38.51891109543246
10	15.53864147385218	27.560176912374633	35.17291486710142	21.728266746671764
11	32.45366188174631	22.752370480755914	24.506781750924784	20.28718588657299
12	20.58613351180625	26.95943807407505	31.85205760367144	20.60237081044726
13	30.65676163975286	21.488663563172157	27.29143725644052	20.563137540634457
14	20.275770323822837	23.025602181237925	28.430026309369165	28.26860118557007
15	21.974381334194927	29.90663141167239	26.081676084849363	22.037311169283317
16	21.53560336812676	28.84844583500926	27.272809695563016	22.34314110130096
17	21.08841827281299	27.19487890436973	27.661680634589896	24.055022188227383
18	22.48824650362331	26.066716340162337	30.251323710742	21.193713445472348
19	22.046624949722073	25.30892078835794	31.31499040598192	21.329463855938073
20	23.07204744917808	25.02307839400753	31.2310839591974	20.673790197616988
21	22.14087546239211	25.71291631774333	31.593085053772658	20.553123166091904
22	20.72687050383431	26.065850900387055	31.245755223959332	21.961523371819304
23	21.8221051451631	26.318147200590808	31.936458587470412	19.923289066775684
24	21.75155119776865	26.04775908794187	29.984356145776324	22.216333568513157
25	21.724846198988505	26.544974844550534	30.67946913099956	21.050709825461404
26	21.431833017928614	27.121811060485175	30.54174057247605	20.904615349110163
27	20.328232458772096	27.381978741502206	30.418518433042983	21.87127036668271
28	21.04836077464278	26.79875475579762	30.410152515215255	21.742731954344343
29	21.03793428592152	26.636134500880278	29.942320499548323	22.383610713649883
30	21.30498427372294	26.996734407247935	31.101103147233562	20.597178171795566
31	22.212336060979712	26.56187152587747	31.020864516639524	20.204927896503293
32	21.563668343696634	26.67829378136189	30.04217576504876	21.71586210989272
33	21.321510052289046	26.71085080147966	30.52447298838827	21.443166157843027
34	20.560788489815835	27.258632967815533	31.22387196107005	20.956706581298587
35	20.211975048959165	29.12555140877111	30.019880387980773	20.642593154288953
36	22.26953750898409	27.04235544683068	29.71289653626275	20.975210507922483
37	21.44617459134758	27.69333100351451	29.69521683799627	21.16527756714164
38	21.204964162551022	26.515879583533895	30.778252899635362	21.500903354279725
39	20.817370777478125	26.157587516566988	31.53452362897855	21.490518076976333
40	19.682078637979124	26.77431638500036	31.144746038758512	22.398858938262
41	21.27003699136868	25.815573959658966	31.08601976829292	21.82836928067943
42	19.91310984656165	27.744062258913203	30.544913851652083	21.797914042873064
43	20.35864648516059	27.60925971105851	29.716399506781748	22.31569429699915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	283.0
2	336.0
3	911.0
4	1486.0
5	1486.0
6	2555.5
7	3625.0
8	3918.5
9	4212.0
10	6582.5
11	8953.0
12	8953.0
13	16284.5
14	23616.0
15	37633.5
16	51651.0
17	50609.0
18	49567.0
19	49567.0
20	53568.5
21	57570.0
22	49477.5
23	41385.0
24	46775.0
25	52165.0
26	52165.0
27	57835.5
28	63506.0
29	68177.5
30	72849.0
31	79658.5
32	86468.0
33	86468.0
34	95098.0
35	103728.0
36	112630.5
37	121533.0
38	127896.5
39	134260.0
40	134260.0
41	137843.0
42	141426.0
43	145332.5
44	149239.0
45	155864.5
46	162490.0
47	162490.0
48	204629.5
49	246769.0
50	228756.5
51	210744.0
52	191196.5
53	171649.0
54	171649.0
55	150487.5
56	129326.0
57	110833.0
58	92340.0
59	82284.0
60	72228.0
61	72228.0
62	63833.5
63	55439.0
64	47914.5
65	40390.0
66	34003.5
67	27617.0
68	27617.0
69	23340.5
70	19064.0
71	15829.5
72	12595.0
73	10286.0
74	7977.0
75	7977.0
76	6346.0
77	4715.0
78	3589.0
79	2463.0
80	1951.5
81	1440.0
82	1440.0
83	1062.5
84	685.0
85	568.0
86	451.0
87	317.0
88	183.0
89	183.0
90	138.5
91	94.0
92	61.0
93	28.0
94	23.0
95	18.0
96	18.0
97	9.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2426512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.804388372768734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67912603465074	36.177943326721575
2	13.207232011591428	12.627272968227606
3	4.669575031433442	6.696785350152841
4	2.0847604293614026	3.986427889174904
5	1.1063676219604859	2.6444613741627827
6	0.662494841137356	1.9002096408411533
7	0.44792696924000913	1.4989012360130642
8	0.32596155108912683	1.2465914066283774
9	0.2371110297602052	1.0201452978711578
>10	1.377825278189351	12.257487817642177
>50	0.12191646017546841	4.0604422878480655
>100	0.07033115044424024	6.196382769566212
>500	0.004165151540791762	1.3709311949704444
>1k	0.004338699521657947	3.653134031779003
>5k	6.074179330321126E-4	1.934710004339617
>10k+	2.603219712994768E-4	2.728173404061062
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23497	0.9683446857052427	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22689	0.9350458600658064	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19582	0.8070019847418847	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8648	0.35639634174485846	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7094	0.2923537983739623	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6939	0.2859660286040209	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6183	0.25481019669385524	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6093	0.2511011690855022	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6014	0.2478454670737256	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5669	0.23362752790837218	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3778	0.15569673671508732	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3446	0.1420145459820516	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3161	0.13026929188893357	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3065	0.12631299577335697	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3047	0.12557119025168637	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2815	0.1160101413057096	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2631	0.10842724041752111	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2500	0.10302854467647389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.121141787058955E-5	0.0
3	0.0	0.0	0.0	8.24228357411791E-5	0.0
4	0.0	0.0	0.0	8.24228357411791E-5	0.0
5	0.0	0.0	0.0	8.24228357411791E-5	4.121141787058955E-5
6	0.0	0.0	0.0	8.24228357411791E-5	4.121141787058955E-5
7	4.121141787058955E-5	0.0	0.0	1.2363425361176867E-4	4.121141787058955E-5
8	1.2363425361176867E-4	0.0	0.0	1.2363425361176867E-4	4.121141787058955E-5
9	1.2363425361176867E-4	0.0	0.0	1.2363425361176867E-4	4.121141787058955E-5
10	1.2363425361176867E-4	0.0	0.0	1.648456714823582E-4	4.121141787058955E-5
11	1.2363425361176867E-4	0.0	0.0	1.648456714823582E-4	4.121141787058955E-5
12	1.2363425361176867E-4	0.0	0.0	3.70902760835306E-4	4.121141787058955E-5
13	1.2363425361176867E-4	0.0	0.0	4.1211417870589556E-4	4.121141787058955E-5
14	1.2363425361176867E-4	0.0	0.0	4.5332559657648507E-4	1.2363425361176867E-4
15	1.2363425361176867E-4	0.0	0.0	6.181712680588433E-4	1.2363425361176867E-4
16	1.2363425361176867E-4	0.0	0.0	8.654397752823807E-4	2.0605708935294778E-4
17	1.2363425361176867E-4	0.0	0.0	0.0013599767897294554	2.0605708935294778E-4
18	1.2363425361176867E-4	0.0	0.0	0.001566033879082403	2.8847992509412686E-4
19	1.648456714823582E-4	0.0	0.0	0.00185451380417653	2.8847992509412686E-4
20	1.648456714823582E-4	0.0	0.0	0.0025551079079765525	2.8847992509412686E-4
21	1.648456714823582E-4	0.0	0.0	0.005110215815953105	3.296913429647164E-4
22	1.648456714823582E-4	0.0	0.0	0.007912592231153195	3.296913429647164E-4
23	1.648456714823582E-4	0.0	0.0	0.01013800879616503	3.70902760835306E-4
24	1.648456714823582E-4	0.0	0.0	0.013929459240259269	3.70902760835306E-4
25	1.648456714823582E-4	0.0	0.0	0.016896681326941717	4.1211417870589556E-4
26	1.648456714823582E-4	0.0	0.0	0.02386141094707135	4.1211417870589556E-4
27	1.648456714823582E-4	0.0	0.0	0.07331511239177882	4.1211417870589556E-4
28	1.648456714823582E-4	0.0	0.0	0.13455527934747488	4.1211417870589556E-4
29	1.648456714823582E-4	0.0	0.0	0.2098897512149126	4.1211417870589556E-4
30	1.648456714823582E-4	0.0	0.0	0.32845500042859876	4.1211417870589556E-4
31	1.648456714823582E-4	0.0	0.0	0.5113100615204046	4.1211417870589556E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6200	0.0	29.361288	1
GTATTGG	730	0.0	19.00685	1
GTATCAA	10615	0.0	17.149317	2
ATTGGAC	760	0.0	16.552631	3
CGAACTA	210	0.0	15.857144	24
GCGAACT	245	0.0	15.857142	23
CGAATTA	165	9.749783E-10	15.69697	15
TATACTG	385	0.0	15.376623	5
GGACCCT	1220	0.0	14.860656	6
TTGGACC	1270	0.0	14.858267	4
AGCGAAC	250	0.0	14.799999	22
ACCGTCG	105	1.6568275E-4	14.095239	23
GTATTAG	305	0.0	13.95082	1
TGGACCC	1370	0.0	13.638686	5
TATACCG	150	1.3070949E-6	13.566667	5
CGTATAC	165	2.59839E-7	13.454545	3
GTCTATA	165	2.59839E-7	13.454545	1
TTGATAC	2090	0.0	13.366029	34
GAACCGT	85	0.009408665	13.058825	6
ACACCGC	185	7.3932824E-8	13.0	6
>>END_MODULE