FastQCFastQC Report
Wed 25 May 2016
SRR2088353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375506
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317720.7261331603704806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT296910.6785729467631858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT269420.6157459274424489No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117390.268288970464216No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA104510.23885237501674092No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG99640.22772223372565367No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA89370.20425066266621505No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG76190.17412843223160934No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72850.1664950293748883No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG72550.16580939438775766No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59590.13618996294371438No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA46940.10727902098637279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74650.029.9370381
GTATTGG13650.017.0769211
GTATCAA132700.016.9246432
TTGGACC21750.015.9057484
ATACGAC2055.456968E-1215.34146319
GGACCCT21600.015.3310186
TGGACCC22100.015.1515845
CGCGATA750.004106853614.80000114
ATTGGAC15150.014.5313523
TACGTTA2750.014.12727219
GTATTAG8350.013.73652651
GACCCTC24300.013.3991787
TACGACT2408.185452E-1113.10416620
GTACGAC1000.001833909412.9500013
TAATACT10000.012.954
TATACAG9200.012.6684795
TTGATAC26650.012.63414634
CTAATAC8500.012.4058833
CGTTGAT29350.012.35434332
ACGACTT2603.092282E-1012.09615421
Produced by FastQC (version 0.11.5)