##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088353_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4375506
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.878495195755644	31.0	31.0	33.0	28.0	34.0
2	31.205130103809708	31.0	31.0	34.0	28.0	34.0
3	31.298259218476673	31.0	31.0	34.0	28.0	34.0
4	35.18580525315244	35.0	35.0	37.0	33.0	37.0
5	34.86924163742434	35.0	35.0	37.0	32.0	37.0
6	34.93847271606987	35.0	35.0	37.0	32.0	37.0
7	34.768436610531445	35.0	35.0	37.0	32.0	37.0
8	34.812900267991864	35.0	35.0	37.0	32.0	37.0
9	36.31806447071493	38.0	35.0	39.0	32.0	39.0
10	36.072335405322264	38.0	35.0	39.0	31.0	39.0
11	36.19402395974317	38.0	35.0	39.0	32.0	39.0
12	35.945175940794044	37.0	35.0	39.0	30.0	39.0
13	36.03475917985257	38.0	35.0	39.0	30.0	39.0
14	36.86834185577622	38.0	36.0	40.0	31.0	41.0
15	37.00295577242952	38.0	36.0	40.0	31.0	41.0
16	36.992554918219746	38.0	36.0	40.0	31.0	41.0
17	37.0133033756553	38.0	36.0	40.0	31.0	41.0
18	37.00198239929279	38.0	36.0	40.0	31.0	41.0
19	37.07528157886196	39.0	36.0	40.0	31.0	41.0
20	37.055669447145085	39.0	36.0	40.0	31.0	41.0
21	36.97130320470364	39.0	36.0	40.0	31.0	41.0
22	36.88792450518866	39.0	36.0	40.0	31.0	41.0
23	36.79064752739455	39.0	35.0	40.0	30.0	41.0
24	36.71546422288073	38.0	35.0	40.0	30.0	41.0
25	36.60278491219073	38.0	35.0	40.0	30.0	41.0
26	36.37790394985174	38.0	35.0	40.0	30.0	41.0
27	36.25059615962131	38.0	35.0	40.0	30.0	41.0
28	36.10777199254212	38.0	34.0	40.0	29.0	41.0
29	35.917564277137316	38.0	34.0	40.0	29.0	41.0
30	35.735664629416576	38.0	34.0	40.0	27.0	41.0
31	35.57911085026509	38.0	34.0	40.0	27.0	41.0
32	35.472131223223094	38.0	34.0	40.0	27.0	41.0
33	35.32338934056998	38.0	34.0	40.0	27.0	41.0
34	35.539220835258824	38.0	34.0	40.0	27.0	41.0
35	35.51504991651251	38.0	34.0	40.0	27.0	41.0
36	35.48422765275605	38.0	34.0	40.0	27.0	41.0
37	35.40392151216339	38.0	34.0	40.0	27.0	41.0
38	35.418022052763725	38.0	34.0	40.0	27.0	41.0
39	35.36998509429538	38.0	34.0	40.0	26.0	41.0
40	35.245091653399626	38.0	34.0	40.0	26.0	41.0
41	35.173317097496835	38.0	34.0	40.0	25.0	41.0
42	35.08693097438331	38.0	33.0	40.0	25.0	41.0
43	34.74993132222879	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	17.0
16	39.0
17	133.0
18	353.0
19	727.0
20	1620.0
21	3342.0
22	6115.0
23	10783.0
24	17918.0
25	28304.0
26	42788.0
27	61958.0
28	86597.0
29	115502.0
30	148590.0
31	184569.0
32	224907.0
33	269891.0
34	324258.0
35	386477.0
36	468222.0
37	581306.0
38	736684.0
39	674400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61226290170782	21.956112047383776	13.084818075897964	24.34680697501043
2	18.38351952894134	23.322331177239843	35.21882954794257	23.07531974587625
3	19.572890541116845	25.307175901484307	31.90780677709047	23.21212678030838
4	13.771687206005431	16.47763710071475	37.09059020830962	32.660085484970196
5	12.712518277886032	38.0399204114907	35.913172099409756	13.334389211213516
6	32.27021057678815	37.74027506761504	14.602059738919337	15.387454616677477
7	25.992742325116225	32.70677722759379	22.624080506346008	18.676399940943973
8	25.898627495882764	34.85242621081996	20.136139683044657	19.11280661025262
9	24.677808692297532	14.339850065341015	21.84647901294159	39.135862229419864
10	15.346156535952643	27.177245328883103	35.601002489769186	21.87559564539507
11	33.227608418317786	22.444901229709206	24.042156495728722	20.285333856244282
12	20.587333213575757	26.394090192082924	31.708355559334166	21.310221035007153
13	30.365128056046547	21.14765697955848	26.878011365999726	21.60920359839525
14	20.662387390166987	22.35576868138222	27.69576821515043	29.28607571330036
15	22.454682955525602	29.504336184203613	25.389703499435267	22.651277360835522
16	22.2489238958877	28.56307361937111	26.5151733308102	22.672829153930994
17	21.434686639670932	26.95370546857895	27.443294558389358	24.168313333360757
18	22.603397184234232	26.133297497478008	29.583664152214624	21.679641166073136
19	22.32907462588327	25.399736624746943	30.27688683320283	21.994301916166954
20	23.232307303429593	25.25858723539632	30.185217435423468	21.323888025750623
21	22.574737641772174	25.890331312538486	30.144170754193915	21.39076029149543
22	21.52953281289067	26.106717714476908	30.04194257761274	22.321806895019687
23	22.362053668764254	26.22690952772091	30.619772890266862	20.791263913247978
24	22.320664170041134	26.022453174558553	28.89992608854839	22.756956566851922
25	22.356340043871498	26.36908736955223	29.601033571888603	21.673539014687673
26	22.052100945582065	26.829125591417313	29.452982123667525	21.665791339333097
27	21.186121102336507	27.00631652659144	29.366157879797218	22.441404491274838
28	21.762877253510794	26.48301704991377	29.491194846950275	22.262910849625165
29	21.78024667318477	26.570184111277644	29.073734557786	22.575834657751585
30	22.001386811033967	26.839958624213978	29.905935450665595	21.252719114086464
31	22.645906553436333	26.464733450256954	29.772533736669544	21.11682625963717
32	22.109442885005755	26.48774793132497	29.113844204533144	22.28896497913613
33	21.86727660755122	26.53233705998803	29.620391333025253	21.979994999435494
34	21.49547960852985	26.892226864732905	30.03252652378948	21.579767002947772
35	21.15012526551215	28.538207923837838	29.040915496402015	21.270751314248
36	22.713441599668702	26.87309764859196	28.79349268404614	21.6199680676932
37	22.073492757180542	27.203642275887635	28.939464372806256	21.78340059412557
38	21.90606069332324	26.414567823698558	29.70113628000967	21.978235202968524
39	21.64506230822218	26.161522804448218	30.27526416373329	21.918150723596312
40	20.654525442314558	26.67220659736268	30.005649632293956	22.667618328028805
41	21.982029049897314	25.81639700642623	29.936011972101056	22.2655619715754
42	20.722540433037917	27.4188173893488	29.601147844386453	22.25749433322683
43	21.13449278780557	27.200762608941687	28.95256000106045	22.712184602192295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	287.0
1	402.0
2	517.0
3	1401.5
4	2286.0
5	2286.0
6	3684.0
7	5082.0
8	5418.0
9	5754.0
10	8969.0
11	12184.0
12	12184.0
13	22273.5
14	32363.0
15	50437.0
16	68511.0
17	69498.5
18	70486.0
19	70486.0
20	78503.0
21	86520.0
22	78933.5
23	71347.0
24	81541.5
25	91736.0
26	91736.0
27	101683.0
28	111630.0
29	122387.5
30	133145.0
31	147120.0
32	161095.0
33	161095.0
34	177552.0
35	194009.0
36	209797.5
37	225586.0
38	235739.0
39	245892.0
40	245892.0
41	252995.0
42	260098.0
43	267762.0
44	275426.0
45	282802.0
46	290178.0
47	290178.0
48	344056.0
49	397934.0
50	376588.0
51	355242.0
52	329887.5
53	304533.0
54	304533.0
55	275139.5
56	245746.0
57	214341.5
58	182937.0
59	166281.0
60	149625.0
61	149625.0
62	133932.0
63	118239.0
64	103295.0
65	88351.0
66	76060.5
67	63770.0
68	63770.0
69	54819.5
70	45869.0
71	39092.5
72	32316.0
73	26702.0
74	21088.0
75	21088.0
76	16618.0
77	12148.0
78	9367.0
79	6586.0
80	5088.0
81	3590.0
82	3590.0
83	2640.0
84	1690.0
85	1308.0
86	926.0
87	686.5
88	447.0
89	447.0
90	352.5
91	258.0
92	158.0
93	58.0
94	36.5
95	15.0
96	15.0
97	10.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4375506.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.23131807414781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85779918108622	33.68312088123183
2	14.255862949115938	13.181346688440756
3	5.404289020745372	7.495422140481123
4	2.5705613539322334	4.753617583310125
5	1.3548515184352659	3.131828574601145
6	0.8060622951793651	2.23591934136089
7	0.5170022614257893	1.6731187195120572
8	0.36303740620428526	1.342695823923513
9	0.25631158493033157	1.0664660168102797
>10	1.4013517445285641	12.048994354865586
>50	0.12445003614788806	3.9821156171605505
>100	0.07921682231404134	6.935734786294469
>500	0.00527354361915892	1.676006730223464
>1k	0.0033830278331260038	3.1684481904789745
>5k	2.9850245586405746E-4	1.0814496937950921
>10k+	2.4875204655338125E-4	2.5437148575101176
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31772	0.7261331603704806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29691	0.6785729467631858	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26942	0.6157459274424489	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11739	0.268288970464216	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10451	0.23885237501674092	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9964	0.22772223372565367	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8937	0.20425066266621505	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7619	0.17412843223160934	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7285	0.1664950293748883	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7255	0.16580939438775766	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5959	0.13618996294371438	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4694	0.10727902098637279	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.2854499571021044E-5	0.0
3	0.0	0.0	0.0	2.2854499571021044E-5	0.0
4	0.0	0.0	0.0	4.570899914204209E-5	0.0
5	0.0	0.0	0.0	4.570899914204209E-5	0.0
6	0.0	0.0	0.0	4.570899914204209E-5	0.0
7	0.0	0.0	0.0	6.856349871306313E-5	0.0
8	0.0	0.0	0.0	6.856349871306313E-5	0.0
9	0.0	0.0	0.0	9.141799828408418E-5	0.0
10	2.2854499571021044E-5	0.0	0.0	1.3712699742612626E-4	0.0
11	2.2854499571021044E-5	0.0	0.0	1.8283599656816835E-4	0.0
12	4.570899914204209E-5	0.0	0.0	2.5139949528123147E-4	0.0
13	4.570899914204209E-5	0.0	0.0	3.199629939942946E-4	0.0
14	4.570899914204209E-5	0.0	0.0	3.4281749356531566E-4	0.0
15	4.570899914204209E-5	0.0	0.0	3.8852649270735776E-4	0.0
16	4.570899914204209E-5	0.0	0.0	7.770529854147155E-4	0.0
17	6.856349871306313E-5	0.0	0.0	9.82743481553905E-4	0.0
18	9.141799828408418E-5	0.0	0.0	0.0011427249785510522	0.0
19	1.1427249785510521E-4	0.0	0.0	0.0014398334729743257	0.0
20	1.1427249785510521E-4	0.0	0.0	0.0020111959622498518	0.0
21	1.1427249785510521E-4	0.0	0.0	0.003588156432650304	0.0
22	1.1427249785510521E-4	0.0	0.0	0.005416516398331987	0.0
23	1.1427249785510521E-4	0.0	0.0	0.006947767869590397	0.0
24	1.1427249785510521E-4	0.0	0.0	0.009301781325405564	0.0
25	1.1427249785510521E-4	0.0	0.0	0.011450104285081543	0.0
26	1.1427249785510521E-4	0.0	0.0	0.01576960470400452	0.0
27	1.1427249785510521E-4	0.0	0.0	0.05053129855152753	0.0
28	1.1427249785510521E-4	0.0	0.0	0.09171510677850744	0.0
29	1.3712699742612626E-4	0.0	0.0	0.141195098349768	0.0
30	1.3712699742612626E-4	0.0	0.0	0.2196088863779412	0.0
31	1.3712699742612626E-4	0.0	0.0	0.33845228414725065	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7465	0.0	29.937038	1
GTATTGG	1365	0.0	17.076921	1
GTATCAA	13270	0.0	16.924643	2
TTGGACC	2175	0.0	15.905748	4
ATACGAC	205	5.456968E-12	15.341463	19
GGACCCT	2160	0.0	15.331018	6
TGGACCC	2210	0.0	15.151584	5
CGCGATA	75	0.0041068536	14.800001	14
ATTGGAC	1515	0.0	14.531352	3
TACGTTA	275	0.0	14.127272	19
GTATTAG	835	0.0	13.7365265	1
GACCCTC	2430	0.0	13.399178	7
TACGACT	240	8.185452E-11	13.104166	20
GTACGAC	100	0.0018339094	12.950001	3
TAATACT	1000	0.0	12.95	4
TATACAG	920	0.0	12.668479	5
TTGATAC	2665	0.0	12.634146	34
CTAATAC	850	0.0	12.405883	3
CGTTGAT	2935	0.0	12.354343	32
ACGACTT	260	3.092282E-10	12.096154	21
>>END_MODULE