FastQCFastQC Report
Wed 25 May 2016
SRR2088352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599520
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT264771.0185341909275558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242120.9314027205022465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT216860.8342309349418354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87590.33694682095156026No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA84430.3247907305964178No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83060.31952052686649846No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA71840.27635871237766974No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG70960.27297347202560474No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA69440.26712623869021973No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG65510.2520080630270204No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46630.17937927001908044No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37020.1424109066289161No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA35090.13498645903859174No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34650.13329383886255924No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34100.13117806364251863No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG32670.12567704807041302No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.12409983381547363No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30810.11852188096263927No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29470.11336708315381301No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28940.11132824521450115No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC28730.11052040376684927No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC27480.10571182372130239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68750.029.3309081
GTATTGG10250.019.1317061
ATTGGAC10100.018.8663373
TTGGACC14450.017.5397914
GTATCAA116000.017.4474122
TGGACCC15200.016.309215
GGACCCT15700.016.0254786
CGTATAC1101.4531915E-515.1363643
TATTGGA12950.015.02
TATCGTG750.00410625914.85
CGCGAAT1406.002738E-714.53571535
ACTAGAC1854.9058144E-914.03
GACCCTC17600.013.8757
CGATCGT1601.7859202E-713.87526
ACGCGAA1203.3035092E-513.87499934
GTATACT2700.013.7037044
TTGTGCG1501.3071985E-613.56666735
CTAGGCA4650.013.1290324
TCTACAC3250.013.0923073
CGTGCTT1703.7363134E-713.05882430
Produced by FastQC (version 0.11.5)