##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2599520
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.844040053548348	31.0	31.0	33.0	28.0	34.0
2	31.16936665230504	31.0	31.0	34.0	28.0	34.0
3	31.238760232658336	31.0	31.0	34.0	28.0	34.0
4	35.13418938881024	35.0	35.0	37.0	32.0	37.0
5	34.831977057302886	35.0	35.0	37.0	32.0	37.0
6	34.91257155167108	35.0	35.0	37.0	32.0	37.0
7	34.742160475780146	35.0	35.0	37.0	31.0	37.0
8	34.80056279620853	35.0	35.0	37.0	32.0	37.0
9	36.28352234258632	38.0	35.0	39.0	32.0	39.0
10	36.053521419338956	38.0	35.0	39.0	30.0	39.0
11	36.17316889271866	38.0	35.0	39.0	31.0	39.0
12	35.93033329230012	37.0	35.0	39.0	30.0	39.0
13	36.00906436572906	38.0	35.0	39.0	30.0	39.0
14	36.79631008801625	38.0	36.0	40.0	31.0	41.0
15	36.93275296977904	38.0	36.0	40.0	31.0	41.0
16	36.909167461685236	38.0	36.0	40.0	31.0	41.0
17	36.97442489382655	38.0	36.0	40.0	31.0	41.0
18	36.96821682464455	38.0	36.0	40.0	31.0	41.0
19	37.04727911306703	39.0	36.0	40.0	31.0	41.0
20	37.04040130485628	39.0	36.0	40.0	31.0	41.0
21	36.950093478796084	39.0	36.0	40.0	31.0	41.0
22	36.8696894041977	39.0	36.0	40.0	31.0	41.0
23	36.76295623807472	39.0	35.0	40.0	30.0	41.0
24	36.68632939927371	38.0	35.0	40.0	30.0	41.0
25	36.566524973841325	38.0	35.0	40.0	30.0	41.0
26	36.33364082599864	38.0	35.0	40.0	30.0	41.0
27	36.2024173693605	38.0	35.0	40.0	30.0	41.0
28	36.051990367452454	38.0	34.0	40.0	29.0	41.0
29	35.849804194620546	38.0	34.0	40.0	29.0	41.0
30	35.640609420200654	38.0	34.0	40.0	27.0	41.0
31	35.43606088816397	38.0	34.0	40.0	27.0	41.0
32	35.32805479473134	38.0	34.0	40.0	27.0	41.0
33	35.16777674339878	38.0	33.0	40.0	27.0	41.0
34	35.3482154397735	38.0	34.0	40.0	27.0	41.0
35	35.28597625715517	38.0	34.0	40.0	27.0	41.0
36	35.242592478611435	38.0	34.0	40.0	26.0	41.0
37	35.14450706284237	38.0	33.0	40.0	26.0	41.0
38	35.132805671816335	38.0	33.0	40.0	25.0	41.0
39	35.053980734904904	38.0	33.0	40.0	25.0	41.0
40	34.90081053425248	38.0	33.0	40.0	25.0	41.0
41	34.79828122114852	38.0	33.0	40.0	24.0	41.0
42	34.67636794485136	38.0	33.0	40.0	24.0	41.0
43	34.323562811595984	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	6.0
16	33.0
17	74.0
18	204.0
19	504.0
20	1117.0
21	2179.0
22	4044.0
23	6967.0
24	11735.0
25	18279.0
26	27346.0
27	39255.0
28	54082.0
29	71687.0
30	91930.0
31	112204.0
32	135028.0
33	163745.0
34	194032.0
35	231335.0
36	280872.0
37	351653.0
38	429620.0
39	371587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14687326891118	22.59328645288361	13.8982581399643	23.361582138240905
2	18.419708253831477	23.66217609404813	35.60876623376623	22.309349418354156
3	19.793654213085492	25.6294238936419	32.58216901581831	21.9947528774543
4	13.747999630701052	16.783175355450236	36.89973533575429	32.569089678094414
5	12.266687696190067	38.47125623191974	35.8398858250754	13.422170246814796
6	31.638494799039822	37.61990675201576	14.194851357173633	16.54674709177079
7	25.03577583553887	33.417823290453626	22.54943220286822	18.996968671139285
8	25.782105927248107	35.14795039084139	19.843355696436266	19.226587985474243
9	24.16792330891857	14.575960177263495	22.681071890195113	38.57504462362282
10	15.582838370160646	27.54466209146304	35.11367483227673	21.75882470609959
11	32.40882932233643	22.65968332615252	24.46178525266203	20.46970209884902
12	20.555756447344127	26.93805010155721	31.745245276050966	20.7609481750477
13	30.80714901212532	21.441112205330214	27.37555394842125	20.376184834123222
14	20.20038314765803	23.101534129377733	28.24309872591863	28.45498399704561
15	21.977480457930696	29.694289715024315	26.222187173016557	22.106042654028435
16	21.38002400443159	28.870868468024867	27.449105988797935	22.300001538745615
17	21.035037237643873	26.965362836215917	27.630985720440698	24.36861420569951
18	22.669069674401428	25.914322644180466	30.230427155782607	21.1861805256355
19	22.00548562811596	25.153643749615313	31.44422816519973	21.396642457069
20	23.120960792761743	24.819697482612174	31.401720317597093	20.65762140702899
21	22.160475780144026	25.552678956114978	31.7490921400874	20.537753123653598
22	20.736251307933774	25.78672216409183	31.379062288422478	22.097964239551917
23	21.830453314458055	26.170908475410844	32.27465070474549	19.723987505385608
24	21.674655320982335	25.87369975995568	30.133216901581832	22.318428017480148
25	21.71408567735582	26.45619191235305	30.78206745860774	21.047654951683388
26	21.415915245891547	26.988444020434542	30.642541700006156	20.953099033667755
27	20.289053363697914	27.187211485197267	30.50974795346833	22.013987197636485
28	21.146519357419834	26.622953468332618	30.484358650827847	21.74616852341971
29	20.99176001723395	26.55017080076322	30.028505262510002	22.42956391949283
30	21.316973902874377	26.75463162429987	31.354596233150733	20.573798239675018
31	22.219371268541884	26.2756970517634	31.18652674339878	20.31840493629593
32	21.68261832953776	26.454653166738474	30.051124823044255	21.811603680679507
33	21.30027851295624	26.573059641780024	30.639387271496275	21.487274573767465
34	20.621537822367205	27.1743244906752	31.24638394780575	20.957753739151844
35	20.301748015018156	29.084715639810426	29.97491844648243	20.638617898688988
36	22.336469809811042	26.872807287499228	29.817235489628857	20.973487413060873
37	21.60706591986213	27.570282205945713	29.67024681479658	21.152405059395583
38	21.525627808210746	26.210838924109066	30.655044008124577	21.60848925955561
39	21.08785468086416	25.988413245522253	31.5012771588601	21.422454914753494
40	19.759263248599744	26.66649996922509	31.018572659567923	22.55566412260725
41	21.606219609774115	25.54683172277959	31.03195974641472	21.814988921031574
42	20.021580907244417	27.755431772019453	30.379031513510185	21.84395580722595
43	20.567258570813074	27.72365667507848	29.378923801317164	22.330160952791285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	262.0
1	306.5
2	351.0
3	871.5
4	1392.0
5	1392.0
6	2405.0
7	3418.0
8	3797.5
9	4177.0
10	6562.5
11	8948.0
12	8948.0
13	16544.5
14	24141.0
15	39298.0
16	54455.0
17	53756.5
18	53058.0
19	53058.0
20	58432.5
21	63807.0
22	54954.0
23	46101.0
24	51687.5
25	57274.0
26	57274.0
27	63190.5
28	69107.0
29	74215.0
30	79323.0
31	87190.5
32	95058.0
33	95058.0
34	102203.5
35	109349.0
36	118877.5
37	128406.0
38	133865.0
39	139324.0
40	139324.0
41	142961.0
42	146598.0
43	151096.0
44	155594.0
45	164228.5
46	172863.0
47	172863.0
48	220202.5
49	267542.0
50	248036.5
51	228531.0
52	207754.0
53	186977.0
54	186977.0
55	162557.5
56	138138.0
57	117541.0
58	96944.0
59	85846.5
60	74749.0
61	74749.0
62	66242.5
63	57736.0
64	50572.0
65	43408.0
66	37336.5
67	31265.0
68	31265.0
69	26859.0
70	22453.0
71	19138.0
72	15823.0
73	13079.0
74	10335.0
75	10335.0
76	8244.5
77	6154.0
78	4678.0
79	3202.0
80	2457.0
81	1712.0
82	1712.0
83	1249.5
84	787.0
85	596.5
86	406.0
87	293.5
88	181.0
89	181.0
90	149.5
91	118.0
92	77.0
93	36.0
94	24.5
95	13.0
96	13.0
97	7.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2599520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.63156936577355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15314483696723	33.09571227481356
2	13.67053983349957	12.20275293692813
3	5.058542256459934	6.773120388266647
4	2.2770239905759597	4.065086167316858
5	1.2301184043362	2.7451057445622893
6	0.7620990210754472	2.0408205193630167
7	0.48232469439236647	1.5068835638218903
8	0.3519990953008786	1.2568217630888558
9	0.25418019986204415	1.021001509939411
>10	1.517070487896141	12.797053817503237
>50	0.1427050911118269	4.4447981899807525
>100	0.08880321989024943	7.310990806966061
>500	0.00546423275632113	1.610790100820042
>1k	0.005117297343220728	4.2647556032617935
>5k	6.071369729244931E-4	2.062621156472707
>10k+	2.602015598247828E-4	2.8016854568945475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26477	1.0185341909275558	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24212	0.9314027205022465	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21686	0.8342309349418354	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8759	0.33694682095156026	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8443	0.3247907305964178	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8306	0.31952052686649846	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7184	0.27635871237766974	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7096	0.27297347202560474	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6944	0.26712623869021973	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6551	0.2520080630270204	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4663	0.17937927001908044	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3702	0.1424109066289161	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3509	0.13498645903859174	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3465	0.13329383886255924	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3410	0.13117806364251863	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3267	0.12567704807041302	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3226	0.12409983381547363	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3081	0.11852188096263927	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2947	0.11336708315381301	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2894	0.11132824521450115	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2873	0.11052040376684927	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2748	0.10571182372130239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.846864036437496E-5	0.0
3	0.0	0.0	0.0	3.846864036437496E-5	0.0
4	0.0	0.0	0.0	3.846864036437496E-5	0.0
5	0.0	0.0	0.0	3.846864036437496E-5	0.0
6	0.0	0.0	0.0	3.846864036437496E-5	0.0
7	0.0	0.0	0.0	3.846864036437496E-5	0.0
8	0.0	0.0	0.0	3.846864036437496E-5	0.0
9	0.0	0.0	0.0	1.1540592109312488E-4	0.0
10	0.0	0.0	0.0	1.5387456145749985E-4	0.0
11	0.0	0.0	0.0	2.3081184218624976E-4	0.0
12	0.0	0.0	0.0	3.077491229149997E-4	0.0
13	3.846864036437496E-5	0.0	0.0	3.8468640364374963E-4	0.0
14	3.846864036437496E-5	0.0	0.0	5.385609651012495E-4	0.0
15	3.846864036437496E-5	0.0	0.0	8.078414476518742E-4	0.0
16	3.846864036437496E-5	0.0	0.0	0.001000184649473749	0.0
17	3.846864036437496E-5	0.0	0.0	0.0014618083338462486	0.0
18	3.846864036437496E-5	0.0	0.0	0.0021157752200406227	3.846864036437496E-5
19	3.846864036437496E-5	0.0	0.0	0.0028082107465993724	3.846864036437496E-5
20	3.846864036437496E-5	0.0	0.0	0.003885332676801871	3.846864036437496E-5
21	7.693728072874992E-5	0.0	0.0	0.0070782298270449925	3.846864036437496E-5
22	7.693728072874992E-5	0.0	0.0	0.012309964916599988	3.846864036437496E-5
23	7.693728072874992E-5	0.0	0.0	0.01550286206684311	3.846864036437496E-5
24	7.693728072874992E-5	0.0	0.0	0.019965224349110605	3.846864036437496E-5
25	7.693728072874992E-5	0.0	0.0	0.023465870622268726	3.846864036437496E-5
26	7.693728072874992E-5	0.0	0.0	0.029813196282390594	3.846864036437496E-5
27	7.693728072874992E-5	0.0	0.0	0.07762971625530868	3.846864036437496E-5
28	7.693728072874992E-5	0.0	0.0	0.1350249276789561	3.846864036437496E-5
29	7.693728072874992E-5	0.0	0.0	0.19934449436819104	3.846864036437496E-5
30	7.693728072874992E-5	0.0	0.0	0.3051332553702222	3.846864036437496E-5
31	7.693728072874992E-5	0.0	0.0	0.4524681479657783	3.846864036437496E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6875	0.0	29.330908	1
GTATTGG	1025	0.0	19.131706	1
ATTGGAC	1010	0.0	18.866337	3
TTGGACC	1445	0.0	17.539791	4
GTATCAA	11600	0.0	17.447412	2
TGGACCC	1520	0.0	16.30921	5
GGACCCT	1570	0.0	16.025478	6
CGTATAC	110	1.4531915E-5	15.136364	3
TATTGGA	1295	0.0	15.0	2
TATCGTG	75	0.004106259	14.8	5
CGCGAAT	140	6.002738E-7	14.535715	35
ACTAGAC	185	4.9058144E-9	14.0	3
GACCCTC	1760	0.0	13.875	7
CGATCGT	160	1.7859202E-7	13.875	26
ACGCGAA	120	3.3035092E-5	13.874999	34
GTATACT	270	0.0	13.703704	4
TTGTGCG	150	1.3071985E-6	13.566667	35
CTAGGCA	465	0.0	13.129032	4
TCTACAC	325	0.0	13.092307	3
CGTGCTT	170	3.7363134E-7	13.058824	30
>>END_MODULE