FastQCFastQC Report
Wed 25 May 2016
SRR2088351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179536
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT386011.2140450682112107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368871.1601378314320077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT325561.023922987505095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132870.41789116399373993No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG127840.4020712456157125No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA124490.3915351170736862No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG116760.36722339360208534No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA114460.3599896337075598No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA110370.34712612154729494No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG107710.33876012097362634No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA68200.2144967064376689No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA59960.1885809753372819No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54990.1729497637391116No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC53110.16703695130358642No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51320.16140719903784703No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50390.15848224395006064No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG49370.15527422869248847No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46820.14725419054855804No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG42660.13417052047845976No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC41190.12954720437195869No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG40920.12869802386260135No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39890.12545855747505297No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35690.1122490828850499No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35650.1121232783651451No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33510.10539273655023877No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA33120.10416614248116705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91550.030.776081
GTATTGG10200.019.9509811
ATTGGAC11200.018.9955353
GTATCAA162650.017.4251462
GGACCCT17950.017.2116996
TTGGACC18350.017.0381474
CCGTATC1059.353405E-615.8571429
GACCCTC20200.015.1113857
CGTATCG1001.0943285E-414.810
TGGACCC20650.014.7820835
GCCGTCA2301.8189894E-1214.47826113
CGACGAG1803.3378456E-914.38888924
GTGGTAT12350.014.3805671
TATTGGA15100.014.211922
CAATGCG1458.9234527E-714.03448319
ACCGTCG1753.5723133E-813.74285823
CGGCTAG950.001245920413.63157912
TTGATAC29600.013.534
CTAGTAC3650.013.1780823
TGATACT11750.013.06808635
Produced by FastQC (version 0.11.5)