##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3179536
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78905790027224	31.0	31.0	33.0	28.0	34.0
2	31.09664240316826	31.0	31.0	34.0	28.0	34.0
3	31.15685496248509	31.0	31.0	34.0	28.0	34.0
4	35.05803079443038	35.0	35.0	37.0	32.0	37.0
5	34.7620221944334	35.0	35.0	37.0	32.0	37.0
6	34.851381144921774	35.0	35.0	37.0	32.0	37.0
7	34.66666299736817	35.0	35.0	37.0	30.0	37.0
8	34.73839673461788	35.0	35.0	37.0	31.0	37.0
9	36.197791124239515	38.0	35.0	39.0	32.0	39.0
10	35.97566720427132	37.0	35.0	39.0	30.0	39.0
11	36.08972441261869	38.0	35.0	39.0	31.0	39.0
12	35.82933075769546	37.0	35.0	39.0	30.0	39.0
13	35.935721124088545	37.0	35.0	39.0	30.0	39.0
14	36.695056133976784	38.0	36.0	40.0	30.0	41.0
15	36.821650706266574	38.0	36.0	40.0	31.0	41.0
16	36.78670409770482	38.0	36.0	40.0	31.0	41.0
17	36.88347576501729	38.0	36.0	40.0	31.0	41.0
18	36.8753928246134	38.0	36.0	40.0	31.0	41.0
19	36.97325899124904	39.0	36.0	40.0	31.0	41.0
20	36.97010003975423	39.0	36.0	40.0	31.0	41.0
21	36.882604254205646	39.0	36.0	40.0	31.0	41.0
22	36.811358953004465	39.0	35.0	40.0	30.0	41.0
23	36.70495065946729	38.0	35.0	40.0	30.0	41.0
24	36.61622198962365	38.0	35.0	40.0	30.0	41.0
25	36.494467746237184	38.0	35.0	40.0	30.0	41.0
26	36.236817258870474	38.0	35.0	40.0	30.0	41.0
27	36.1059717518531	38.0	34.0	40.0	30.0	41.0
28	35.94455983514576	38.0	34.0	40.0	29.0	41.0
29	35.733957407621745	38.0	34.0	40.0	28.0	41.0
30	35.51656845527146	38.0	34.0	40.0	27.0	41.0
31	35.2826336924633	38.0	34.0	40.0	27.0	41.0
32	35.18081034465406	37.0	33.0	40.0	27.0	41.0
33	34.996250710795536	37.0	33.0	40.0	26.0	41.0
34	35.151488456177255	38.0	33.0	40.0	27.0	41.0
35	35.07058451296038	38.0	33.0	40.0	26.0	41.0
36	34.99555752789086	38.0	33.0	40.0	25.0	41.0
37	34.88519865791738	38.0	33.0	40.0	25.0	41.0
38	34.84342904121859	38.0	33.0	40.0	25.0	41.0
39	34.74043571137424	38.0	33.0	40.0	24.0	41.0
40	34.55395001031597	38.0	33.0	40.0	24.0	41.0
41	34.43413064044565	38.0	33.0	40.0	23.0	41.0
42	34.28283749578555	38.0	33.0	40.0	23.0	41.0
43	33.92854051660368	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	16.0
16	29.0
17	120.0
18	310.0
19	719.0
20	1506.0
21	2883.0
22	5471.0
23	9535.0
24	15525.0
25	23967.0
26	35624.0
27	51606.0
28	70536.0
29	93714.0
30	118904.0
31	143519.0
32	171920.0
33	204668.0
34	242082.0
35	285138.0
36	343101.0
37	425900.0
38	507653.0
39	425087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.379225144801	22.57908072121215	14.306835966002588	22.73485816798426
2	18.626648668233354	23.905437774568362	35.55248312961388	21.915430427584404
3	19.990967235470837	25.370399957729685	33.05381665752487	21.58481614927461
4	13.75854841712753	16.513101282702884	36.4129231435027	33.315427156666885
5	12.045405367324037	38.705741969897495	35.630639187604736	13.618213475173736
6	31.834016032528016	37.2453716517127	13.667591749236365	17.253020566522913
7	24.730463816103985	34.051540853759796	21.99122765082704	19.22676767930918
8	26.186053562532397	34.72402891491085	19.603898178853772	19.48601934370298
9	23.92358507656463	14.552343486596786	22.987096230393366	38.53697520644522
10	15.761796689831472	27.51851213510399	34.750888179910525	21.96880299515401
11	32.20649176483612	22.375057241056556	24.761065765570827	20.65738522853649
12	20.910818433884693	26.8859670090227	31.815176805672273	20.38803775142033
13	31.39219055862239	21.242250441573866	27.65051252761409	19.715046472189655
14	20.209646942195338	23.12950694692559	28.604645457702006	28.056200653177065
15	21.52675736333855	29.66803961332723	26.88986694913975	21.915336074194474
16	21.128082839760268	28.93695809703051	27.675799236114955	22.25915982709427
17	20.88858248499152	26.72282999783616	27.681932206460313	24.706655310712
18	22.786123509845464	25.35426552805189	30.905106908681013	20.954504053421633
19	22.017394990967233	24.514394553167506	32.13616703820935	21.33204341765591
20	23.229490089119924	24.00067179613629	32.27838904796172	20.49144906678207
21	22.26271380478158	24.76524876585766	32.817492866883725	20.154544562477042
22	20.30120118155605	25.119860256339287	32.32496817145646	22.253970390648195
23	21.745185461023244	25.552376195771963	33.44550903024844	19.256929312956355
24	21.68143402056149	25.261358890102205	30.688282818625108	22.368924270711197
25	21.385038571665802	26.090913894354394	31.642289944193113	20.881757589786687
26	21.205861484191406	26.66933162574665	31.55010668223288	20.574700207829068
27	19.690483139678243	26.88571539998289	31.37747142979353	22.046330030545338
28	20.63351382088456	26.138405100618456	31.382220550419937	21.84586052807705
29	20.670500349736564	25.973475375023273	30.607516316846233	22.74850795839393
30	21.016840193034454	26.36475888305715	32.31996744179025	20.298433482118146
31	22.292466573739063	25.8054948898204	32.0042295479592	19.897808988481337
32	21.492978849744112	25.989263842271328	30.69844153360742	21.81931577437714
33	21.121478102465264	25.984766330684728	31.520196657625515	21.373558909224492
34	20.06122905983766	26.85039578101962	32.26319815218321	20.825177006959507
35	19.611886765867727	29.257948329567583	30.694006924280774	20.436157980283916
36	22.302279326291636	26.411526713331757	30.45563881019117	20.830555150185436
37	21.362676818252726	27.520933872112156	30.23145515572083	20.88493415391428
38	21.1847263248474	25.80128043840359	31.558881547496238	21.45511168925277
39	20.64209997936806	25.465917039467396	32.40865962832313	21.48332335284142
40	19.13291750746021	26.232129467947523	31.815271159062203	22.819681865530065
41	21.208346123459524	25.13417052047846	31.808414812727392	21.849068543334624
42	19.53335329431716	27.653657640611712	31.12127052500742	21.691718540063707
43	20.13328359861313	27.79462160516503	29.834164481861503	22.237930314360334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	232.0
1	355.5
2	479.0
3	1225.0
4	1971.0
5	1971.0
6	3409.5
7	4848.0
8	5499.5
9	6151.0
10	9608.0
11	13065.0
12	13065.0
13	24146.5
14	35228.0
15	57717.5
16	80207.0
17	78427.5
18	76648.0
19	76648.0
20	81801.5
21	86955.0
22	71572.5
23	56190.0
24	61006.0
25	65822.0
26	65822.0
27	71804.0
28	77786.0
29	82520.5
30	87255.0
31	93770.0
32	100285.0
33	100285.0
34	111240.0
35	122195.0
36	132756.0
37	143317.0
38	150730.0
39	158143.0
40	158143.0
41	163875.5
42	169608.0
43	177313.5
44	185019.0
45	199255.5
46	213492.0
47	213492.0
48	290363.0
49	367234.0
50	334787.0
51	302340.0
52	271206.5
53	240073.0
54	240073.0
55	206883.5
56	173694.0
57	144115.0
58	114536.0
59	100874.5
60	87213.0
61	87213.0
62	76694.0
63	66175.0
64	57130.0
65	48085.0
66	40900.0
67	33715.0
68	33715.0
69	28731.5
70	23748.0
71	19983.0
72	16218.0
73	13022.0
74	9826.0
75	9826.0
76	7720.0
77	5614.0
78	4309.5
79	3005.0
80	2328.0
81	1651.0
82	1651.0
83	1221.5
84	792.0
85	598.0
86	404.0
87	302.5
88	201.0
89	201.0
90	145.5
91	90.0
92	55.0
93	20.0
94	11.5
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3179536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.19163723843098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18079457917463	32.039899695222786
2	13.603810908476824	11.751417316382824
3	5.101234450413591	6.60992003551352
4	2.2788602328874226	3.937108179838395
5	1.290020198814648	2.7859042228725444
6	0.7596886422725845	1.9687317750716176
7	0.514923930947166	1.5568285341599817
8	0.3594930530796908	1.2421674830683216
9	0.2600278543112914	1.010792587977079
>10	1.4090190743677342	11.449592699204505
>50	0.13595296678491028	4.0822419792400035
>100	0.09337718240989772	7.5314824904934135
>500	0.005996273925211289	1.7346570384722249
>1k	0.0056306474662689366	5.183662596441153
>5k	4.3875175061835873E-4	1.067444811342235
>10k+	7.312529176972645E-4	6.048148554699233
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38601	1.2140450682112107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36887	1.1601378314320077	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32556	1.023922987505095	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13287	0.41789116399373993	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12784	0.4020712456157125	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12449	0.3915351170736862	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11676	0.36722339360208534	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11446	0.3599896337075598	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11037	0.34712612154729494	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10771	0.33876012097362634	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6820	0.2144967064376689	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5996	0.1885809753372819	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5499	0.1729497637391116	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5311	0.16703695130358642	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5132	0.16140719903784703	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5039	0.15848224395006064	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4937	0.15527422869248847	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4682	0.14725419054855804	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4266	0.13417052047845976	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4119	0.12954720437195869	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4092	0.12869802386260135	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3989	0.12545855747505297	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3569	0.1122490828850499	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3565	0.1121232783651451	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3351	0.10539273655023877	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3312	0.10416614248116705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.145112997619779E-5	0.0	0.0	0.0	0.0
3	3.145112997619779E-5	0.0	0.0	0.0	0.0
4	3.145112997619779E-5	0.0	0.0	0.0	0.0
5	3.145112997619779E-5	0.0	0.0	0.0	0.0
6	3.145112997619779E-5	0.0	0.0	0.0	0.0
7	3.145112997619779E-5	0.0	0.0	0.0	0.0
8	2.2015790983338448E-4	0.0	0.0	0.0	0.0
9	2.2015790983338448E-4	0.0	0.0	6.290225995239558E-5	0.0
10	2.2015790983338448E-4	0.0	0.0	2.2015790983338448E-4	0.0
11	2.2015790983338448E-4	0.0	0.0	5.032180796191646E-4	0.0
12	2.2015790983338448E-4	0.0	0.0	8.177293793811424E-4	0.0
13	2.516090398095823E-4	0.0	0.0	8.806316393335379E-4	0.0
14	5.661203395715601E-4	0.0	0.0	0.0010378872892145268	0.0
15	6.290225995239557E-4	0.0	0.0	0.0013523985889765048	0.0
16	6.604737295001535E-4	0.0	0.0	0.0019814211885004606	0.0
17	6.604737295001535E-4	0.0	0.0	0.0027676994379054053	0.0
18	7.23375989452549E-4	0.0	0.0	0.003365270907453163	0.0
19	7.23375989452549E-4	0.0	0.0	0.004088646896905712	0.0
20	7.548271194287469E-4	0.0	0.0	0.0061015192153823705	0.0
21	7.548271194287469E-4	0.0	0.0	0.010001459332430896	0.0
22	7.548271194287469E-4	0.0	0.0	0.016920707927194408	0.0
23	7.548271194287469E-4	0.0	0.0	0.02160692629364788	0.0
24	7.548271194287469E-4	0.0	0.0	0.02670200934979192	0.0
25	7.548271194287469E-4	0.0	0.0	0.032017250315769345	0.0
26	7.548271194287469E-4	0.0	0.0	0.039974386199747386	0.0
27	7.548271194287469E-4	0.0	0.0	0.10586450349988175	0.0
28	8.491805093573402E-4	0.0	0.0	0.18681971205861483	0.0
29	8.491805093573402E-4	0.0	0.0	0.2777449288198026	0.0
30	0.0010693384191907246	0.0	0.0	0.43883761655788767	0.0
31	0.0011322406791431203	0.0	0.0	0.6539947967250568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9155	0.0	30.77608	1
GTATTGG	1020	0.0	19.950981	1
ATTGGAC	1120	0.0	18.995535	3
GTATCAA	16265	0.0	17.425146	2
GGACCCT	1795	0.0	17.211699	6
TTGGACC	1835	0.0	17.038147	4
CCGTATC	105	9.353405E-6	15.857142	9
GACCCTC	2020	0.0	15.111385	7
CGTATCG	100	1.0943285E-4	14.8	10
TGGACCC	2065	0.0	14.782083	5
GCCGTCA	230	1.8189894E-12	14.478261	13
CGACGAG	180	3.3378456E-9	14.388889	24
GTGGTAT	1235	0.0	14.380567	1
TATTGGA	1510	0.0	14.21192	2
CAATGCG	145	8.9234527E-7	14.034483	19
ACCGTCG	175	3.5723133E-8	13.742858	23
CGGCTAG	95	0.0012459204	13.631579	12
TTGATAC	2960	0.0	13.5	34
CTAGTAC	365	0.0	13.178082	3
TGATACT	1175	0.0	13.068086	35
>>END_MODULE