FastQCFastQC Report
Wed 25 May 2016
SRR2088350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452343
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87621.9370256641530872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68481.5138954289112467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48951.0821434177162021No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.6532653318388921No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG23780.5257072619671356No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA19410.42909915705559726No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG18730.41406631693206264No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12490.2761179016808042No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA11930.2637379156967169No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11580.2560004244566623No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.23588294723252043No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC9510.2102386905511968No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.19874298928025855No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7750.1713301631726367No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC7630.16867730903318942No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG7270.16071874661484759No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7170.15850803483197484No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA6460.14281198117357846No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6370.14082234056899298No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC6340.1401591270341312No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.1341902052203748No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5940.13131627990264025No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG5920.13087413754606572No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA5530.12225236159286205No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5050.11164094503507294No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA5020.11097773150021112No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA4880.1078827350041893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.029.8029921
CCGGAGG502.698592E-422.232
TACACTT502.698592E-422.25
GTGTTAA450.003822274720.5555551
CTATAGT450.003822274720.5555554
ATACTTA450.003822274720.5555556
AGTGCGC450.003822274720.5555558
GTATCAA29600.020.18752
CCATACA555.1364687E-420.1818182
CTTACCA656.890307E-519.9230779
CGAACTA609.225011E-418.524
CCTGTGC500.00702844718.58
ACTTACC609.225011E-418.58
GTGTACT500.00702844718.54
ACTTTAC500.00702844718.58
TACACCG500.00702844718.55
CATACAC752.0637753E-417.2666663
GATACCC1553.9835868E-1016.70967936
TATTCTC904.436372E-516.4444455
TAGAGTC803.376895E-416.18755
Produced by FastQC (version 0.11.5)