##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452343
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.22333715786472	31.0	30.0	31.0	27.0	34.0
2	30.479465803604786	31.0	30.0	31.0	27.0	34.0
3	30.403072447235836	31.0	30.0	33.0	27.0	34.0
4	34.270447425957734	35.0	35.0	37.0	30.0	37.0
5	33.88406806339437	35.0	33.0	37.0	28.0	37.0
6	34.177608584636	35.0	35.0	37.0	30.0	37.0
7	34.00859966883537	35.0	33.0	37.0	30.0	37.0
8	34.182189179450106	35.0	35.0	37.0	30.0	37.0
9	35.499119031354525	37.0	35.0	39.0	30.0	39.0
10	35.190945808822065	37.0	34.0	39.0	29.0	39.0
11	35.42126439449709	37.0	34.0	39.0	30.0	39.0
12	35.101270496061616	37.0	34.0	39.0	29.0	39.0
13	35.27374580793778	37.0	34.0	39.0	30.0	39.0
14	35.876036547487196	38.0	34.0	40.0	27.0	41.0
15	36.033364062227115	38.0	34.0	40.0	29.0	41.0
16	35.89396763075808	38.0	34.0	40.0	28.0	41.0
17	36.055659090557384	38.0	34.0	40.0	29.0	41.0
18	36.080682137227726	38.0	34.0	40.0	29.0	41.0
19	36.198997221135286	38.0	34.0	40.0	29.0	41.0
20	36.18335643527146	38.0	34.0	40.0	29.0	41.0
21	36.08018251636479	38.0	34.0	40.0	29.0	41.0
22	35.98079996816575	38.0	34.0	40.0	29.0	41.0
23	35.81822422365329	38.0	34.0	40.0	28.0	41.0
24	35.73695624780311	38.0	34.0	40.0	27.0	41.0
25	35.59578019334885	38.0	34.0	40.0	27.0	41.0
26	35.230800520843694	37.0	33.0	40.0	27.0	41.0
27	35.041338099627936	37.0	33.0	40.0	27.0	41.0
28	34.82848855846117	37.0	33.0	40.0	26.0	41.0
29	34.63862820912449	37.0	33.0	40.0	25.0	41.0
30	34.37357491991697	37.0	33.0	39.0	25.0	40.0
31	34.05937087564083	36.0	32.0	39.0	24.0	40.0
32	33.907727100894675	36.0	32.0	39.0	24.0	40.0
33	33.66601008526715	36.0	32.0	39.0	24.0	40.0
34	33.744081371879304	36.0	32.0	39.0	24.0	40.0
35	33.666151570821256	36.0	32.0	39.0	23.0	40.0
36	33.54954978854542	36.0	32.0	39.0	23.0	40.0
37	33.383832622589495	36.0	31.0	39.0	21.0	40.0
38	33.24995633844229	36.0	31.0	39.0	21.0	40.0
39	33.04027253654859	36.0	31.0	39.0	19.0	40.0
40	32.802189931092116	36.0	31.0	39.0	18.0	40.0
41	32.63471967069237	36.0	31.0	39.0	16.0	40.0
42	32.411267555814945	36.0	30.0	39.0	15.0	40.0
43	31.998271223385792	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	5.0
15	7.0
16	18.0
17	51.0
18	139.0
19	264.0
20	539.0
21	966.0
22	1600.0
23	2597.0
24	3992.0
25	5795.0
26	8003.0
27	10764.0
28	13954.0
29	17832.0
30	21604.0
31	25241.0
32	29580.0
33	34541.0
34	39341.0
35	45598.0
36	53886.0
37	59256.0
38	53338.0
39	23430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14917883110825	25.292974579025206	14.908598121337127	19.64924846852941
2	23.211368364272246	25.985590580599236	30.884085749088637	19.918955306039884
3	25.2063146771366	25.47137901990304	31.078186243624863	18.244120059335504
4	19.17925114349067	18.58655931450249	32.378969056667174	29.855220485339668
5	11.946465403465954	41.330583207875435	33.79139281474456	12.931558573914042
6	32.060405488755215	36.823162953776226	12.98527887023785	18.13115268723071
7	23.66390106622629	35.279643986974484	22.03858576345826	19.01786918334096
8	26.856832094229375	36.04875061623591	18.94823176218047	18.14618552735424
9	24.121739476459236	14.564832439100417	23.28564828017677	38.02777980426358
10	17.093665647528535	28.847356983527987	32.93231021592022	21.12666715302326
11	32.22731422836211	22.501066668435236	23.99418140658748	21.277437696615177
12	19.329800615904304	28.583176925474696	32.63121127109295	19.45581118752805
13	31.9859045016724	21.15474319266574	28.51309736195763	18.346254943704224
14	19.32184205348596	24.02004673444709	29.76391808870702	26.894193123359926
15	21.862170963185015	29.802605544907294	28.01259221431524	20.322631277592446
16	19.9430520644732	29.08832456786111	29.89545543978795	21.07316792787774
17	19.97687595475115	27.48821137941783	29.53776227331914	22.997150392511877
18	21.17839780874248	25.55140678644303	32.081849393049076	21.188346011765407
19	21.206031706028387	24.586431093219083	34.33080648976551	19.87673071098702
20	21.90793269709048	24.09145272503388	35.065204944035834	18.935409633839807
21	20.98142338888852	24.910521440588226	35.411181338055414	18.69687383246784
22	19.15758616801852	24.89570967164298	35.01303214596003	20.93367201437847
23	20.413933674225092	25.36813877964288	36.16680262544131	18.051124920690715
24	20.56558850253016	25.347800231240452	33.48719887342128	20.599412392808112
25	20.248351361687924	26.669142663863482	33.844670968711796	19.237835005736798
26	20.435598649697244	26.59663131738526	33.94945870721997	19.018311325697535
27	19.249330707007736	26.74563329155088	33.79050853003142	20.214527471409973
28	19.5053311314644	26.575850626626256	34.23729338134999	19.681524860559353
29	19.599507453414777	25.402846954633983	33.877389503098314	21.12025608885293
30	20.0854219032902	26.166868946794796	34.8728730189259	18.874836130989095
31	20.940746292083663	26.001065563079344	34.219828758265294	18.838359386571696
32	20.9661694775867	25.947566337933825	33.19538491808208	19.890879266397402
33	20.41658652836454	26.353895163625836	33.363399013580405	19.866119294429225
34	19.231645012744753	26.823008203951424	34.52424377076687	19.421103012536946
35	18.947789619823897	28.48855846116774	33.303488724264554	19.26016319474381
36	21.53122740928897	25.750591918079863	33.06937434645833	19.64880632617284
37	20.44068328679785	26.87916028323639	32.8544931611631	19.825663268802657
38	20.67236588164291	25.131150476518926	33.71622861412689	20.480255027711273
39	19.85771858965431	25.292311365490345	34.33080648976551	20.519163555089833
40	18.41058665658582	26.030689100969838	33.25043164147561	22.308292600968734
41	20.75040400757832	25.032331659824514	33.06318435346628	21.15407997913088
42	19.46376974994639	27.313565148570884	31.749137269726734	21.47352783175599
43	20.150858972063233	27.114822159290625	30.721156290690914	22.013162577955224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	46.5
2	59.0
3	142.0
4	225.0
5	225.0
6	514.0
7	803.0
8	940.5
9	1078.0
10	1735.5
11	2393.0
12	2393.0
13	4979.0
14	7565.0
15	11827.5
16	16090.0
17	14814.0
18	13538.0
19	13538.0
20	15657.0
21	17776.0
22	13804.0
23	9832.0
24	10059.0
25	10286.0
26	10286.0
27	10820.0
28	11354.0
29	11796.5
30	12239.0
31	13228.5
32	14218.0
33	14218.0
34	15442.5
35	16667.0
36	18006.0
37	19345.0
38	20523.0
39	21701.0
40	21701.0
41	22822.5
42	23944.0
43	24825.5
44	25707.0
45	27735.5
46	29764.0
47	29764.0
48	40923.5
49	52083.0
50	46228.0
51	40373.0
52	35592.5
53	30812.0
54	30812.0
55	26304.0
56	21796.0
57	18424.5
58	15053.0
59	13128.0
60	11203.0
61	11203.0
62	9818.5
63	8434.0
64	7228.5
65	6023.0
66	5152.0
67	4281.0
68	4281.0
69	3614.5
70	2948.0
71	2417.5
72	1887.0
73	1571.0
74	1255.0
75	1255.0
76	1006.0
77	757.0
78	574.0
79	391.0
80	303.5
81	216.0
82	216.0
83	164.5
84	113.0
85	84.0
86	55.0
87	38.5
88	22.0
89	22.0
90	19.0
91	16.0
92	10.5
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	452343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.39930112889087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.34759233013926	52.7573353203168
2	7.309122293416364	8.829317567758888
3	2.004994291612684	3.633007619424653
4	0.9031735647015353	2.1820420842424735
5	0.5258498786570647	1.5880482584799387
6	0.34883143577152387	1.2641504959432577
7	0.23887778022859216	1.0099635686719428
8	0.1841759567497252	0.8899279257942587
9	0.14293460950250855	0.7769835468974197
>10	0.8894729880132117	9.874744210084682
>50	0.05799311067216221	2.498697490567648
>100	0.03743859042658905	4.847974156310056
>500	0.005505675062733684	2.239538820516014
>1k	0.00330340503764021	4.147647178086923
>5k	7.340900083644911E-4	3.4606217569050655
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8762	1.9370256641530872	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6848	1.5138954289112467	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4895	1.0821434177162021	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2955	0.6532653318388921	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2378	0.5257072619671356	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1941	0.42909915705559726	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1873	0.41406631693206264	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1249	0.2761179016808042	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1193	0.2637379156967169	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1158	0.2560004244566623	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1067	0.23588294723252043	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	951	0.2102386905511968	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	899	0.19874298928025855	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	775	0.1713301631726367	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	763	0.16867730903318942	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	727	0.16071874661484759	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	717	0.15850803483197484	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	646	0.14281198117357846	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	637	0.14082234056899298	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	634	0.1401591270341312	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	607	0.1341902052203748	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	594	0.13131627990264025	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	592	0.13087413754606572	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	553	0.12225236159286205	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	505	0.11164094503507294	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	502	0.11097773150021112	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	488	0.1078827350041893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.421423565745463E-4	0.0	0.0	0.0	0.0
6	4.421423565745463E-4	0.0	0.0	0.0	0.0
7	4.421423565745463E-4	0.0	0.0	0.0	0.0
8	4.421423565745463E-4	0.0	0.0	0.0	0.0
9	4.421423565745463E-4	0.0	0.0	0.0	0.0
10	4.421423565745463E-4	0.0	0.0	4.421423565745463E-4	0.0
11	4.421423565745463E-4	0.0	0.0	4.421423565745463E-4	0.0
12	4.421423565745463E-4	0.0	0.0	4.421423565745463E-4	0.0
13	4.421423565745463E-4	0.0	0.0	4.421423565745463E-4	0.0
14	4.421423565745463E-4	0.0	0.0	8.842847131490926E-4	0.0
15	4.421423565745463E-4	0.0	0.0	0.0011053558914363658	0.0
16	4.421423565745463E-4	0.0	0.0	0.0019896406045854585	0.0
17	4.421423565745463E-4	0.0	0.0	0.002652854139447278	0.0
18	4.421423565745463E-4	0.0	0.0	0.002652854139447278	0.0
19	4.421423565745463E-4	0.0	0.0	0.0033160676743090975	0.0
20	4.421423565745463E-4	0.0	0.0	0.005968921813756375	0.0
21	4.421423565745463E-4	0.0	0.0	0.01260105716237457	0.0
22	4.421423565745463E-4	0.0	0.0	0.021443904293865494	0.0
23	4.421423565745463E-4	0.0	0.0	0.024981043146461865	0.0
24	4.421423565745463E-4	0.0	0.0	0.032718534386516424	0.0
25	4.421423565745463E-4	0.0	0.0	0.0386874562002728	0.0
26	4.421423565745463E-4	0.0	0.0	0.046867089796901906	0.0
27	4.421423565745463E-4	0.0	0.0	0.10235595554700747	0.0
28	4.421423565745463E-4	0.0	0.0	0.17000373610291306	0.0
29	4.421423565745463E-4	0.0	0.0	0.2535686414955023	0.0
30	4.421423565745463E-4	0.0	0.0	0.39726490738222986	0.0
31	4.421423565745463E-4	0.0	0.0	0.6030821743676812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2005	0.0	29.802992	1
CCGGAGG	50	2.698592E-4	22.2	32
TACACTT	50	2.698592E-4	22.2	5
GTGTTAA	45	0.0038222747	20.555555	1
CTATAGT	45	0.0038222747	20.555555	4
ATACTTA	45	0.0038222747	20.555555	6
AGTGCGC	45	0.0038222747	20.555555	8
GTATCAA	2960	0.0	20.1875	2
CCATACA	55	5.1364687E-4	20.181818	2
CTTACCA	65	6.890307E-5	19.923077	9
CGAACTA	60	9.225011E-4	18.5	24
CCTGTGC	50	0.007028447	18.5	8
ACTTACC	60	9.225011E-4	18.5	8
GTGTACT	50	0.007028447	18.5	4
ACTTTAC	50	0.007028447	18.5	8
TACACCG	50	0.007028447	18.5	5
CATACAC	75	2.0637753E-4	17.266666	3
GATACCC	155	3.9835868E-10	16.709679	36
TATTCTC	90	4.436372E-5	16.444445	5
TAGAGTC	80	3.376895E-4	16.1875	5
>>END_MODULE