FastQCFastQC Report
Wed 25 May 2016
SRR2088349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418479
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT390021.14091676444407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT380141.1120150218854643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323670.9468245965530284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140460.41088448985645376No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA110570.32344794278391065No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG109990.32175128178350665No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG108000.3159299793855689No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA103440.3025907135892893No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG97800.286092147999154No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93770.2743032793239333No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA63830.186720468372045No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA61090.17870520778392962No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC54090.15822826467560572No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51890.1517926539844182No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.1446842294482429No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47060.1376635632396747No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46960.1373710354809844No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG40680.11900029223523093No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG39800.11642604795875591No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38760.11338375926837638No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.11116054830232978No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37530.10978566783648519No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35180.10291126550726215No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC34370.10054179066187037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96550.030.121181
GTATTGG8700.020.201151
CGCGATA1103.8582584E-818.514
CAATGCG2400.018.519
GTCGTAC500.007037655518.4999981
GTATCAA166250.017.5040592
CGAACTA1655.4569682E-1116.81818224
ATTGGAC10300.016.5242713
ATCGTCA2450.015.10204110
TTGGACC16150.014.7770884
ATTAGAC3550.014.591553
TTAGACT5200.014.5865384
GGACCCT16600.014.4879526
CGCCGGA1803.3378456E-914.38888814
TGCGACG2600.014.23076922
GACCCTC16750.014.0268677
GCGAACT2009.858923E-1013.87499923
CGACGAA3100.013.72580710
TATTGGA13000.013.6615382
AGCCGTC1907.1413524E-913.63157912
Produced by FastQC (version 0.11.5)