##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3418479
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.796860826115942	31.0	31.0	33.0	28.0	34.0
2	31.10928164250826	31.0	31.0	34.0	28.0	34.0
3	31.17222513287342	31.0	31.0	34.0	28.0	34.0
4	35.07106464600192	35.0	35.0	37.0	32.0	37.0
5	34.77347527950296	35.0	35.0	37.0	32.0	37.0
6	34.85796139160135	35.0	35.0	37.0	32.0	37.0
7	34.67677554842373	35.0	35.0	37.0	31.0	37.0
8	34.73898362400354	35.0	35.0	37.0	31.0	37.0
9	36.201832744913744	38.0	35.0	39.0	32.0	39.0
10	35.97734489520047	37.0	35.0	39.0	30.0	39.0
11	36.09789909488986	38.0	35.0	39.0	31.0	39.0
12	35.84260309921459	37.0	35.0	39.0	30.0	39.0
13	35.93806573040232	37.0	35.0	39.0	30.0	39.0
14	36.70299744418497	38.0	36.0	40.0	30.0	41.0
15	36.83806336092748	38.0	36.0	40.0	31.0	41.0
16	36.81579439276942	38.0	36.0	40.0	31.0	41.0
17	36.896948321168566	38.0	36.0	40.0	31.0	41.0
18	36.8972490397045	38.0	36.0	40.0	31.0	41.0
19	36.99267100953377	39.0	36.0	40.0	31.0	41.0
20	36.98408151695535	39.0	36.0	40.0	31.0	41.0
21	36.896407437342745	39.0	36.0	40.0	31.0	41.0
22	36.82688060976826	39.0	35.0	40.0	30.0	41.0
23	36.718994324668955	38.0	35.0	40.0	30.0	41.0
24	36.63551684828253	38.0	35.0	40.0	30.0	41.0
25	36.51657944951541	38.0	35.0	40.0	30.0	41.0
26	36.266756940732996	38.0	35.0	40.0	30.0	41.0
27	36.121762046805024	38.0	34.0	40.0	30.0	41.0
28	35.96324827503694	38.0	34.0	40.0	29.0	41.0
29	35.76165072244118	38.0	34.0	40.0	28.0	41.0
30	35.54973220546331	38.0	34.0	40.0	27.0	41.0
31	35.3227037521658	38.0	34.0	40.0	27.0	41.0
32	35.20656350382729	37.0	34.0	40.0	27.0	41.0
33	35.03552486354312	37.0	33.0	40.0	26.0	41.0
34	35.19452686414046	38.0	33.0	40.0	27.0	41.0
35	35.13185747228519	38.0	33.0	40.0	26.0	41.0
36	35.06222065427344	38.0	33.0	40.0	26.0	41.0
37	34.94901621452114	38.0	33.0	40.0	25.0	41.0
38	34.91056314811353	38.0	33.0	40.0	25.0	41.0
39	34.81310138222291	38.0	33.0	40.0	24.0	41.0
40	34.62774731101171	38.0	33.0	40.0	24.0	41.0
41	34.51103721859926	38.0	33.0	40.0	23.0	41.0
42	34.36481166039049	38.0	33.0	40.0	23.0	41.0
43	34.00216061002569	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	7.0
15	14.0
16	43.0
17	133.0
18	344.0
19	755.0
20	1518.0
21	3209.0
22	5837.0
23	9969.0
24	16316.0
25	25657.0
26	37798.0
27	54510.0
28	75306.0
29	99535.0
30	125592.0
31	153033.0
32	183360.0
33	219765.0
34	259551.0
35	305848.0
36	370117.0
37	456875.0
38	545749.0
39	467637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.036285143187946	22.754242456952348	14.349217883157978	22.860254516701726
2	18.59631140047957	24.144773157886885	35.32073767309964	21.9381777685339
3	19.789532128177473	25.38055667447423	33.05654356806053	21.773367629287762
4	13.693341395398361	16.88113339295049	36.644747561708	32.78077764994315
5	12.07759357304813	38.77610481152583	35.605513446184695	13.540788169241349
6	31.442463153934835	37.737894543157935	13.99028632324493	16.8293559796623
7	24.589122823337515	34.10174524986112	22.29494462303264	19.014187303768722
8	26.029675771008097	34.81469390334122	19.852718124054586	19.30291220159609
9	24.001493061680357	14.557058855707467	23.085413132565684	38.3560349500465
10	15.59304591310931	27.747925320003432	34.96844649330887	21.690582273578396
11	32.064289410582894	22.73320971110251	24.742378116115383	20.460122762199212
12	20.6485983971234	27.27324052597661	31.9111511289085	20.16700994799149
13	31.137239690517333	21.521793756814066	27.488014406407057	19.85295214626154
14	19.94044719888582	23.508642293838868	28.749394101879812	27.8015164053955
15	21.55996862932316	29.987342323881467	26.74809469357571	21.704594353219665
16	20.99053409425654	29.046543799157458	27.81933134589974	22.14359076068626
17	20.782312835620754	27.132856454581116	27.924846108459345	24.15998460133878
18	22.46425968976261	25.811742590783798	30.943176775402158	20.780820944051435
19	21.726621693449047	25.2318940675078	32.21292276477345	20.828561474269698
20	22.887927642673834	24.712686548608314	32.123906567803985	20.275479240913867
21	21.85469034620368	25.47214711572018	32.64364063666911	20.02952190140703
22	20.182367655322732	25.78804784232988	32.20900289280701	21.820581609540383
23	21.518400434813262	26.117697373598027	33.026822747777594	19.337079443811124
24	21.2997651879681	25.96271616704388	30.75859175966855	21.978926885319467
25	21.144813234189826	26.656826032864323	31.60525485164601	20.59310588129984
26	20.851963694964926	27.229273603845456	31.446090498142592	20.472672203047026
27	19.613956967411532	27.348888204373935	31.250769713665054	21.786385114549482
28	20.468781583856448	26.779453669307312	31.20583744993022	21.545927296906022
29	20.41934439263778	26.626812684822692	30.639854742416144	22.31398818012338
30	20.87387402409083	26.96836809586954	32.17229065909137	19.985467220948262
31	21.888974599522186	26.547537662217614	31.75400521694005	19.809482521320156
32	21.179945818008537	26.737329672055903	30.72963151155821	21.35309299837735
33	20.86088579160498	26.565996163790974	31.364767781226682	21.20835026337737
34	19.922690763933318	27.316768656469737	32.171968878556804	20.58857170104014
35	19.482056201018054	29.424343399506036	30.814640078233623	20.278960321242284
36	21.812946635038564	26.9761493342507	30.538757149012763	20.672146881697973
37	21.065450453257135	27.88942099688195	30.284170240624558	20.76095830923636
38	20.831077212994433	26.25500990352727	31.634917166377207	21.278995717101086
39	20.428471258708917	25.910880248203956	32.44457549688034	21.21607299620679
40	19.088577112803677	26.45182257957413	32.03916127611139	22.420439031510796
41	20.80363810922928	25.48785585636185	32.024242360418185	21.684263673990685
42	19.320610130996858	27.716917377582252	31.406628503495266	21.555843987925623
43	19.77546154298447	27.755355525074165	30.311550838837974	22.157632093103395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	307.0
1	413.5
2	520.0
3	1348.0
4	2176.0
5	2176.0
6	3750.0
7	5324.0
8	5938.5
9	6553.0
10	10473.5
11	14394.0
12	14394.0
13	26539.5
14	38685.0
15	62209.5
16	85734.0
17	84152.5
18	82571.0
19	82571.0
20	89000.0
21	95429.0
22	81187.0
23	66945.0
24	73858.0
25	80771.0
26	80771.0
27	87903.5
28	95036.0
29	99599.0
30	104162.0
31	112996.0
32	121830.0
33	121830.0
34	132067.0
35	142304.0
36	153018.0
37	163732.0
38	168729.0
39	173726.0
40	173726.0
41	178172.0
42	182618.0
43	187599.5
44	192581.0
45	207411.0
46	222241.0
47	222241.0
48	295120.0
49	367999.0
50	336925.5
51	305852.0
52	274325.5
53	242799.0
54	242799.0
55	211057.0
56	179315.0
57	151085.5
58	122856.0
59	108207.5
60	93559.0
61	93559.0
62	81754.0
63	69949.0
64	60697.5
65	51446.0
66	44183.0
67	36920.0
68	36920.0
69	31016.0
70	25112.0
71	21583.5
72	18055.0
73	14827.0
74	11599.0
75	11599.0
76	9363.5
77	7128.0
78	5563.0
79	3998.0
80	3075.5
81	2153.0
82	2153.0
83	1636.0
84	1119.0
85	865.5
86	612.0
87	421.5
88	231.0
89	231.0
90	162.0
91	93.0
92	62.0
93	31.0
94	21.0
95	11.0
96	11.0
97	7.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3418479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.10342912757003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.2392011875682	30.415180874330506
2	14.171048147633062	11.932994426945022
3	5.494274829656584	6.939834326935136
4	2.743101564126904	4.619759291797744
5	1.434387761202717	3.0196321722626216
6	0.9122447138183087	2.3045178393149737
7	0.5722550803838337	1.686573085388284
8	0.37677483165545606	1.26908099373261
9	0.27704374896263706	1.0498042664716223
>10	1.529671908090144	12.080700662022476
>50	0.14243194097671574	4.171736143663127
>100	0.09391777882864055	7.336712782692177
>500	0.007068190855543076	1.9996620996605183
>1k	0.0055985670137468435	5.0193092121149165
>5k	4.198925260310133E-4	1.2448018848611901
>10k+	5.598567013746844E-4	4.909699937807069
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39002	1.14091676444407	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38014	1.1120150218854643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32367	0.9468245965530284	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14046	0.41088448985645376	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11057	0.32344794278391065	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10999	0.32175128178350665	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10800	0.3159299793855689	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10344	0.3025907135892893	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9780	0.286092147999154	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9377	0.2743032793239333	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6383	0.186720468372045	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6109	0.17870520778392962	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5409	0.15822826467560572	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5189	0.1517926539844182	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4946	0.1446842294482429	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4706	0.1376635632396747	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4696	0.1373710354809844	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4068	0.11900029223523093	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3980	0.11642604795875591	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3876	0.11338375926837638	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3800	0.11116054830232978	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3753	0.10978566783648519	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3518	0.10291126550726215	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3437	0.10054179066187037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.925277586903415E-5	0.0	0.0	0.0	0.0
3	2.925277586903415E-5	0.0	0.0	5.85055517380683E-5	0.0
4	2.925277586903415E-5	0.0	0.0	8.775832760710245E-5	0.0
5	5.85055517380683E-5	2.925277586903415E-5	0.0	8.775832760710245E-5	0.0
6	1.170111034761366E-4	2.925277586903415E-5	0.0	8.775832760710245E-5	0.0
7	1.170111034761366E-4	2.925277586903415E-5	0.0	8.775832760710245E-5	0.0
8	1.755166552142049E-4	2.925277586903415E-5	0.0	8.775832760710245E-5	0.0
9	1.755166552142049E-4	2.925277586903415E-5	0.0	1.755166552142049E-4	0.0
10	1.755166552142049E-4	2.925277586903415E-5	0.0	2.925277586903415E-4	0.0
11	1.755166552142049E-4	8.775832760710245E-5	0.0	4.095388621664781E-4	0.0
12	1.755166552142049E-4	8.775832760710245E-5	0.0	6.435610691187514E-4	0.0
13	2.0476943108323906E-4	8.775832760710245E-5	0.0	7.60572172594888E-4	0.0
14	2.0476943108323906E-4	8.775832760710245E-5	0.0	9.068360519400588E-4	0.0
15	2.0476943108323906E-4	8.775832760710245E-5	0.0	0.0012871221382375027	0.0
16	2.0476943108323906E-4	8.775832760710245E-5	0.0	0.0018429248797491517	0.0
17	2.0476943108323906E-4	8.775832760710245E-5	0.0	0.0024864859488679027	0.0
18	2.0476943108323906E-4	8.775832760710245E-5	0.0	0.0028082664834272785	0.0
19	2.0476943108323906E-4	8.775832760710245E-5	0.0	0.003481080328415064	0.0
20	2.0476943108323906E-4	8.775832760710245E-5	0.0	0.005206994104688079	0.0
21	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.008834338312448314	0.0
22	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.013251507468672472	0.0
23	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.016878851676432705	0.0
24	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.022904923505453742	0.0
25	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.028141170386010855	0.0
26	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.03791159752626826	0.0
27	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.11192112047492467	0.0
28	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.1971637093572902	0.0
29	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.29317131975946026	0.0
30	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.45470514810826684	0.0
31	2.0476943108323906E-4	1.170111034761366E-4	0.0	0.6849829997493037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9655	0.0	30.12118	1
GTATTGG	870	0.0	20.20115	1
CGCGATA	110	3.8582584E-8	18.5	14
CAATGCG	240	0.0	18.5	19
GTCGTAC	50	0.0070376555	18.499998	1
GTATCAA	16625	0.0	17.504059	2
CGAACTA	165	5.4569682E-11	16.818182	24
ATTGGAC	1030	0.0	16.524271	3
ATCGTCA	245	0.0	15.102041	10
TTGGACC	1615	0.0	14.777088	4
ATTAGAC	355	0.0	14.59155	3
TTAGACT	520	0.0	14.586538	4
GGACCCT	1660	0.0	14.487952	6
CGCCGGA	180	3.3378456E-9	14.388888	14
TGCGACG	260	0.0	14.230769	22
GACCCTC	1675	0.0	14.026867	7
GCGAACT	200	9.858923E-10	13.874999	23
CGACGAA	310	0.0	13.725807	10
TATTGGA	1300	0.0	13.661538	2
AGCCGTC	190	7.1413524E-9	13.631579	12
>>END_MODULE