FastQCFastQC Report
Wed 25 May 2016
SRR2088348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466076
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82651.7733159398896317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67781.4542692608072503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47251.013783159828011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.5722242724362551No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20940.449282949561874No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA18960.40680060762622405No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG17200.3690385259056463No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG12210.2619744419365082No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA10660.22871806314849938No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.20447309022562843No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9450.20275663196560217No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.18709395034286255No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC8490.18215913284528704No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA6880.14761541036225853No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC6450.13838944721461735No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA6250.13409830156455171No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG5760.12358499472189086No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC5690.12208309374436786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.1205811927668449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5610.12036663548434161No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG5590.11993752091933504No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA5220.11199890146671358No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA5070.10878054222916433No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG4950.10620585483912495No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT4880.10470395386160197No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA4810.10320205288407899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.031.62571
CTGCTCG250.00549303229.69
GTGTAAC358.861452E-426.428571
CACTATA451.3215638E-424.6666662
GTAGTGT551.8995139E-523.5454546
GTATCAA26550.021.3917142
CTAGACT701.2172246E-418.54
TAGACTG500.007028760418.55
CTAACAC701.2172246E-418.53
AACTGGT609.22559E-418.57
GACTAGT500.007028760418.515
CATACAG953.597088E-617.5263165
GTGGTAT2050.017.1463431
GTGTTAG650.001578028917.0769231
CTGTGCC650.001578028917.0769239
ACAGTGT904.4368382E-516.4444458
ATACTGG904.4368382E-516.4444456
ACACCAT803.3771695E-416.18756
AACAGCG700.002589393915.8571427
TTGGACC1659.677024E-1015.696974
Produced by FastQC (version 0.11.5)