##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088348_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466076
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.20139204764888	31.0	30.0	31.0	27.0	34.0
2	30.440250087968487	31.0	30.0	31.0	27.0	34.0
3	30.35689458371596	31.0	30.0	31.0	27.0	34.0
4	34.24583973429226	35.0	35.0	37.0	30.0	37.0
5	33.85973746770913	35.0	33.0	37.0	28.0	37.0
6	34.15479878818047	35.0	33.0	37.0	30.0	37.0
7	33.97427672740068	35.0	33.0	37.0	30.0	37.0
8	34.1224113663866	35.0	33.0	37.0	30.0	37.0
9	35.45995502879359	37.0	35.0	39.0	30.0	39.0
10	35.130429372033745	37.0	34.0	39.0	29.0	39.0
11	35.3775800513221	37.0	34.0	39.0	30.0	39.0
12	35.04385336297084	37.0	34.0	39.0	29.0	39.0
13	35.218803371124025	37.0	34.0	39.0	29.0	39.0
14	35.827485217003236	38.0	34.0	40.0	27.0	41.0
15	35.97115921008591	38.0	34.0	40.0	29.0	41.0
16	35.84615384615385	38.0	34.0	40.0	27.0	41.0
17	35.99187900685725	38.0	34.0	40.0	29.0	41.0
18	36.01247650597757	38.0	34.0	40.0	29.0	41.0
19	36.13324007243454	38.0	34.0	40.0	29.0	41.0
20	36.10050077669736	38.0	34.0	40.0	29.0	41.0
21	36.014710047288425	38.0	34.0	40.0	29.0	41.0
22	35.91928569589509	38.0	34.0	40.0	29.0	41.0
23	35.7520146928827	38.0	34.0	40.0	27.0	41.0
24	35.675565787553964	38.0	34.0	40.0	27.0	41.0
25	35.52841811206756	38.0	34.0	40.0	27.0	41.0
26	35.18309031145135	37.0	33.0	40.0	27.0	41.0
27	34.97190157828337	37.0	33.0	40.0	27.0	41.0
28	34.774796385138906	37.0	33.0	40.0	26.0	41.0
29	34.57803448364644	37.0	33.0	39.0	25.0	41.0
30	34.320353332932825	37.0	33.0	39.0	25.0	40.0
31	34.050390923368724	36.0	32.0	39.0	24.0	40.0
32	33.89784713222736	36.0	32.0	39.0	24.0	40.0
33	33.6765034028785	36.0	32.0	39.0	24.0	40.0
34	33.79976441610381	36.0	32.0	39.0	24.0	40.0
35	33.713239900788714	36.0	32.0	39.0	24.0	40.0
36	33.59224890361229	36.0	32.0	39.0	23.0	40.0
37	33.433540881744605	36.0	31.0	39.0	22.0	40.0
38	33.33467074039427	36.0	31.0	39.0	21.0	40.0
39	33.16231687535938	36.0	31.0	39.0	20.0	40.0
40	32.9544280331963	36.0	31.0	39.0	18.0	40.0
41	32.77362919352208	36.0	31.0	39.0	18.0	40.0
42	32.57842068675495	36.0	30.0	39.0	16.0	40.0
43	32.17075112213459	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	6.0
15	10.0
16	23.0
17	66.0
18	139.0
19	324.0
20	523.0
21	1018.0
22	1689.0
23	2688.0
24	4089.0
25	5857.0
26	8217.0
27	11202.0
28	14650.0
29	18286.0
30	22395.0
31	26172.0
32	30559.0
33	35363.0
34	41000.0
35	46882.0
36	55201.0
37	61072.0
38	55255.0
39	23389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.541619821659985	25.525879899415543	14.968159699276512	19.964340579647953
2	23.291265802143858	25.96228941202722	30.843467588976903	19.902977196852014
3	24.81891365356723	25.491550734215018	31.331585406671874	18.357950205545876
4	19.052686686291505	19.107613350612347	32.53374986053777	29.30595010255838
5	11.978947639440777	41.21430839605558	33.95669375809954	12.850050206404106
6	31.61372823316369	37.61189162282546	13.260498287832887	17.513881856177964
7	23.915842051510914	35.35067242252337	22.124717857173508	18.608767668792215
8	26.7516456543568	35.99198413992568	19.170264077103305	18.08610612861422
9	24.330366721307254	14.354954985882133	23.00268625717694	38.31199203563367
10	16.661874887357428	29.04633579072941	33.35335867970031	20.938430642212857
11	32.45608012427158	22.61197744573846	23.94909843029892	20.982843999691035
12	19.485663282383133	28.727718226212033	32.345797681064894	19.440820810339947
13	31.718432187025293	21.323990078871258	28.08125713402964	18.876320600073807
14	19.419365082089616	24.03749603069027	29.767677374505446	26.775461512714667
15	22.164840069001624	29.84878002729169	27.649353324350535	20.337026579356156
16	20.06475338785949	29.116496022107984	29.573932148404978	21.244818441627547
17	20.267080905260087	27.796325062865286	29.40786481174744	22.52872922012719
18	21.273569117482985	25.59947304731417	31.863043795432507	21.263914039770338
19	21.39479398209734	24.67451660244252	34.17232382701534	19.758365588444804
20	21.993837914846505	24.347960418472525	34.47871162643003	19.179490040250947
21	21.066735897149822	25.26905483225912	34.836164059080495	18.82804521151057
22	19.386537817866614	25.43619495532917	34.4636926166548	20.71357461014942
23	20.691689767334083	25.573511616131274	35.285447008642365	18.449351607892275
24	20.631613728233162	25.553343231575965	33.110479835906595	20.70456320428428
25	20.57132313184974	26.52807696598838	33.54946403590831	19.35113586625357
26	20.195204215621487	26.84583630137574	33.41536573434375	19.543593748659017
27	19.525141822363736	26.82952994790549	33.23857053356105	20.406757696169723
28	19.73133137084939	26.78533114770982	33.59044447686643	19.89289300457436
29	19.852985350028753	25.754168847999036	33.24758193942619	21.145263862546024
30	20.255065697439903	26.57013019335902	34.27767145272445	18.897132656476625
31	21.104068864305393	26.449119886027173	33.364730215672985	19.082081033994456
32	20.75305315013002	26.290562054257244	32.923385885563725	20.032998910049006
33	20.480350844068347	26.551034595216233	33.051262025935685	19.917352534779738
34	19.485448725100625	26.95290038534488	34.0521717488135	19.509479140740996
35	19.1767007955784	28.41360636462723	32.94055046816399	19.469142371630376
36	21.514731503016677	26.199160651910848	32.44320668732138	19.842901157751097
37	20.63933779040328	27.16488298045812	32.225216488298045	19.97056274084055
38	20.525193316111533	25.64431551935736	33.27525982886911	20.555231335661993
39	19.823161887760797	25.682506715642944	33.91163672877385	20.582694667822416
40	18.8104515143453	26.03051862786327	32.93969223903398	22.219337618757457
41	20.582050995974903	25.368394854058135	32.82640599387224	21.223148156094716
42	19.627700203400302	27.348973128845937	31.568242089272996	21.45508457848076
43	20.310206919043246	27.037435954651173	30.644787545378865	22.007569580926717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	71.0
2	72.0
3	146.0
4	220.0
5	220.0
6	458.5
7	697.0
8	829.5
9	962.0
10	1588.0
11	2214.0
12	2214.0
13	4604.0
14	6994.0
15	11141.5
16	15289.0
17	14159.5
18	13030.0
19	13030.0
20	15191.0
21	17352.0
22	13804.5
23	10257.0
24	10690.5
25	11124.0
26	11124.0
27	11818.0
28	12512.0
29	13172.5
30	13833.0
31	14865.0
32	15897.0
33	15897.0
34	17339.0
35	18781.0
36	20260.0
37	21739.0
38	22774.0
39	23809.0
40	23809.0
41	24566.0
42	25323.0
43	26030.0
44	26737.0
45	28549.0
46	30361.0
47	30361.0
48	40151.5
49	49942.0
50	44940.5
51	39939.0
52	34904.0
53	29869.0
54	29869.0
55	25930.5
56	21992.0
57	18641.5
58	15291.0
59	13669.0
60	12047.0
61	12047.0
62	10629.0
63	9211.0
64	8122.5
65	7034.0
66	5872.5
67	4711.0
68	4711.0
69	3953.0
70	3195.0
71	2668.5
72	2142.0
73	1781.5
74	1421.0
75	1421.0
76	1194.0
77	967.0
78	746.5
79	526.0
80	404.5
81	283.0
82	283.0
83	202.5
84	122.0
85	90.0
86	58.0
87	43.5
88	29.0
89	29.0
90	24.0
91	19.0
92	11.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.58255409855117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.55264881504216	53.30133178025235
2	7.990485610950706	9.841490248201328
3	2.1682050551192957	4.005708153109085
4	0.9370587394060446	2.308258820519716
5	0.5303053905995168	1.632878020267402
6	0.3451015486786532	1.2751340874598167
7	0.24482366034474473	1.0553806415449891
8	0.17999404363018628	0.886759434341834
9	0.1257795913749648	0.6971245641308186
>10	0.8275935080197302	9.444090163208337
>50	0.05371039744172479	2.2914957560217957
>100	0.03627195666766744	4.698113986151964
>500	0.004882763397570616	2.0260267546571944
>1k	0.002441381698785308	3.3052608637145737
>5k	6.975376282243738E-4	3.230946726418762
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8265	1.7733159398896317	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6778	1.4542692608072503	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4725	1.013783159828011	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2667	0.5722242724362551	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2094	0.449282949561874	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1896	0.40680060762622405	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1720	0.3690385259056463	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1221	0.2619744419365082	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1066	0.22871806314849938	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.20447309022562843	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	945	0.20275663196560217	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	0.18709395034286255	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	849	0.18215913284528704	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	688	0.14761541036225853	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	645	0.13838944721461735	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	625	0.13409830156455171	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	576	0.12358499472189086	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	569	0.12208309374436786	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.1205811927668449	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	561	0.12036663548434161	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	559	0.11993752091933504	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	522	0.11199890146671358	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	507	0.10878054222916433	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	495	0.10620585483912495	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	488	0.10470395386160197	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	481	0.10320205288407899	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.1455728250328272E-4	0.0
16	0.0	0.0	0.0	4.2911456500656543E-4	0.0
17	0.0	0.0	0.0	4.2911456500656543E-4	0.0
18	0.0	0.0	0.0	6.436718475098482E-4	0.0
19	0.0	0.0	0.0	0.0010727864125164137	0.0
20	0.0	0.0	0.0	0.001501900977522979	0.0
21	2.1455728250328272E-4	0.0	0.0	0.0034329165200525234	0.0
22	2.1455728250328272E-4	0.0	0.0	0.006651275757601764	0.0
23	2.1455728250328272E-4	0.0	0.0	0.009011405865137875	0.0
24	2.1455728250328272E-4	0.0	0.0	0.014589895210223225	0.0
25	2.1455728250328272E-4	0.0	0.0	0.01823736901277903	0.0
26	2.1455728250328272E-4	0.0	0.0	0.02445953020537423	0.0
27	2.1455728250328272E-4	0.0	0.0	0.07874252267870475	0.0
28	2.1455728250328272E-4	0.0	0.0	0.13924767634463048	0.0
29	2.1455728250328272E-4	0.0	0.0	0.204902204790635	0.0
30	2.1455728250328272E-4	0.0	0.0	0.3239814965799569	0.0
31	2.1455728250328272E-4	0.0	0.0	0.48039375552485003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	31.6257	1
CTGCTCG	25	0.005493032	29.6	9
GTGTAAC	35	8.861452E-4	26.42857	1
CACTATA	45	1.3215638E-4	24.666666	2
GTAGTGT	55	1.8995139E-5	23.545454	6
GTATCAA	2655	0.0	21.391714	2
CTAGACT	70	1.2172246E-4	18.5	4
TAGACTG	50	0.0070287604	18.5	5
CTAACAC	70	1.2172246E-4	18.5	3
AACTGGT	60	9.22559E-4	18.5	7
GACTAGT	50	0.0070287604	18.5	15
CATACAG	95	3.597088E-6	17.526316	5
GTGGTAT	205	0.0	17.146343	1
GTGTTAG	65	0.0015780289	17.076923	1
CTGTGCC	65	0.0015780289	17.076923	9
ACAGTGT	90	4.4368382E-5	16.444445	8
ATACTGG	90	4.4368382E-5	16.444445	6
ACACCAT	80	3.3771695E-4	16.1875	6
AACAGCG	70	0.0025893939	15.857142	7
TTGGACC	165	9.677024E-10	15.69697	4
>>END_MODULE