FastQCFastQC Report
Wed 25 May 2016
SRR2088347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551832
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37740.6839037968077241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37360.6770176430507836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31770.5757186969947375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14920.2703721422461909No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG7540.13663578770350396No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG7370.13355513997013582No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA6780.12286348018962293No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG6300.11416518070717174No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA5880.10655416866002697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA358.8635273E-426.42857212
GGTATCA9400.025.3882961
TTGCGTG601.3353019E-624.6666667
GACCGTC400.001929921423.12500222
GTATACG400.001929921423.1250021
CGAAGAG400.001929921423.1250025
CGTGCGG502.6996364E-422.19999910
TTGGACC957.1249815E-921.4210534
CGACGCT450.003823324320.55555728
AGTGCGA450.003823324320.55555711
TGCGGTC555.138438E-420.18181812
GTGCGGT555.138438E-420.18181811
GCCGTCA555.138438E-420.18181813
GGAGTGT656.8938236E-519.9230756
CGCCGGA656.8938236E-519.92307514
TATACCG656.8938236E-519.9230755
TACCGAC951.6721788E-719.4736847
GGATACA609.2285214E-418.51
TCGAACT609.2285214E-418.519
TGCGTGC701.21773985E-418.58