##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551832
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02726192029458	31.0	31.0	33.0	30.0	34.0
2	31.36890575392511	31.0	31.0	34.0	30.0	34.0
3	31.471438771220228	31.0	31.0	34.0	30.0	34.0
4	35.34411197610867	37.0	35.0	37.0	33.0	37.0
5	35.07878665970803	35.0	35.0	37.0	32.0	37.0
6	35.11305614752316	37.0	35.0	37.0	32.0	37.0
7	34.9483719682802	36.0	35.0	37.0	32.0	37.0
8	34.98334094434538	36.0	35.0	37.0	32.0	37.0
9	36.522050189188015	38.0	35.0	39.0	32.0	39.0
10	36.288741863465695	38.0	35.0	39.0	32.0	39.0
11	36.40181613244611	38.0	35.0	39.0	32.0	39.0
12	36.17267755403819	38.0	35.0	39.0	31.0	39.0
13	36.252138331956104	38.0	35.0	39.0	32.0	39.0
14	37.12655663317821	39.0	36.0	41.0	31.0	41.0
15	37.26663006132301	39.0	36.0	41.0	32.0	41.0
16	37.25826519665405	39.0	36.0	41.0	32.0	41.0
17	37.27140506530973	39.0	36.0	41.0	32.0	41.0
18	37.26757056495455	39.0	36.0	40.0	32.0	41.0
19	37.32774286377013	39.0	36.0	41.0	31.0	41.0
20	37.305411791994665	39.0	36.0	40.0	31.0	41.0
21	37.23091629336465	39.0	36.0	40.0	31.0	41.0
22	37.146299960857654	39.0	36.0	40.0	31.0	41.0
23	37.03783216631149	39.0	36.0	40.0	31.0	41.0
24	36.95945867582888	39.0	36.0	40.0	31.0	41.0
25	36.83739072761275	39.0	35.0	40.0	30.0	41.0
26	36.622935966018645	38.0	35.0	40.0	30.0	41.0
27	36.49291994665043	38.0	35.0	40.0	30.0	41.0
28	36.378319850969135	38.0	35.0	40.0	30.0	41.0
29	36.17124958320648	38.0	35.0	40.0	30.0	41.0
30	36.00681366792792	38.0	34.0	40.0	29.0	41.0
31	35.8558202496412	38.0	34.0	40.0	29.0	41.0
32	35.715339088708156	38.0	34.0	40.0	28.0	41.0
33	35.5551870859247	38.0	34.0	40.0	27.0	41.0
34	35.77431718349063	38.0	34.0	40.0	29.0	41.0
35	35.74949803563403	38.0	34.0	40.0	28.0	41.0
36	35.71994375099668	38.0	34.0	40.0	28.0	41.0
37	35.64387530987692	38.0	34.0	40.0	27.0	41.0
38	35.65496926600849	38.0	34.0	40.0	27.0	41.0
39	35.59260970730222	38.0	34.0	40.0	27.0	41.0
40	35.458208295278276	38.0	34.0	40.0	26.0	41.0
41	35.40710216152742	38.0	34.0	40.0	26.0	41.0
42	35.33298540135404	38.0	34.0	40.0	26.0	41.0
43	34.976027124197216	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	6.0
17	11.0
18	31.0
19	78.0
20	175.0
21	313.0
22	691.0
23	1173.0
24	1920.0
25	3050.0
26	4643.0
27	6861.0
28	9561.0
29	13188.0
30	17028.0
31	21377.0
32	26484.0
33	32565.0
34	39360.0
35	47975.0
36	59327.0
37	74683.0
38	96112.0
39	95217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71335478913872	22.695856710013192	13.631141361863754	23.959647138984327
2	18.213151828817466	23.457682773017872	35.47220893315357	22.85695646501109
3	19.11306339610606	25.405376998796736	32.378513750561766	23.103045854535438
4	13.826490671073804	16.932870873744182	37.2867104481074	31.953928007074616
5	12.86732193856101	38.191877237999975	35.568615085750736	13.372185737688284
6	31.49454906565766	38.29227011119326	15.198466199857927	15.014714623291148
7	25.165811333884225	33.186911958712074	23.250010872874352	18.39726583452935
8	25.25823076588527	35.327599704257814	20.481052204294063	18.933117325562854
9	24.734339436640138	14.661164992244016	22.38634222009597	38.21815335101987
10	15.345431218196842	27.886566926165933	35.42581800258049	21.342183853056728
11	32.708324272604706	23.339168442569477	24.06674495136201	19.88576233346381
12	20.06154046883834	27.137244668667275	32.01372881601647	20.787486046477913
13	29.740935647080995	21.97806578813842	26.659563055422662	21.62143550935792
14	20.18494759274562	23.194377999101174	28.406652749387497	28.21402165876571
15	22.46643906116354	30.03704025862944	25.48964177503298	22.006878905174037
16	21.87785412951768	28.816197683352907	26.82175009785587	22.484198089273548
17	21.002044100378374	27.829665550384902	27.982973078763102	23.185317270473625
18	21.86969947375288	27.11586134910625	29.646522854781892	21.36791632235898
19	22.018476637817308	26.4779860537265	30.64247814552255	20.861059162933646
20	22.567375578074486	26.30546978065788	30.25649110598878	20.87066353527885
21	21.82330854317981	26.928304266515894	30.264464547181024	20.98392264312327
22	21.49204830455646	27.202481914785658	29.962198640165848	21.343271140492035
23	21.805549515069803	27.0653024833645	30.244712158772955	20.884435842792733
24	21.63140231084823	27.191427825860043	29.452442047579698	21.72472781571203
25	21.889089433015847	27.26699430261384	29.813783905246527	21.03013235912379
26	21.455986604618797	27.758629437944883	29.5039069861842	21.28147697125212
27	21.03303179228461	27.781824903231417	29.517316864552978	21.66782643993099
28	21.28800069586396	27.705714782759973	29.49176560982328	21.51451891155279
29	21.13831745893678	27.46596790327491	29.48034909175256	21.91536554603575
30	21.400716159990722	27.857572594557762	30.075820177155364	20.665891068296148
31	22.01249655692312	27.36213195320315	29.84839588860378	20.77697560126995
32	21.324787254091827	27.6511691964221	29.547398483596453	21.476645065889617
33	21.264805230577423	27.360501022050187	29.918888357326146	21.455805390046244
34	20.995882804911638	27.427731628466635	30.382616448484324	21.193769118137403
35	20.83623276649415	28.34141550326911	29.74872787370069	21.07362385653605
36	21.85067194363502	27.384602560199482	29.480167877180012	21.284557618985488
37	21.37317154496296	27.323895678394873	29.6146290900129	21.688303686629265
38	21.153358268458515	26.946969367488656	30.245799446208267	21.65387291784456
39	20.765196654054133	26.553008886762637	30.895272474231287	21.786521984951943
40	20.182954232447557	26.660650342857974	30.96159700778498	22.194798416909496
41	20.909624668377333	26.0620986097218	30.988054335377434	22.040222386523432
42	19.868184519926356	27.3623131677757	30.50783571811711	22.261666594180838
43	20.291320546833095	26.890792849997823	30.25286681453776	22.56501978863132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	86.5
2	107.0
3	241.5
4	376.0
5	376.0
6	616.5
7	857.0
8	922.5
9	988.0
10	1485.0
11	1982.0
12	1982.0
13	3316.5
14	4651.0
15	7019.0
16	9387.0
17	9381.5
18	9376.0
19	9376.0
20	10669.0
21	11962.0
22	11136.0
23	10310.0
24	11981.5
25	13653.0
26	13653.0
27	15429.5
28	17206.0
29	18294.0
30	19382.0
31	21210.0
32	23038.0
33	23038.0
34	25086.0
35	27134.0
36	28702.5
37	30271.0
38	31386.5
39	32502.0
40	32502.0
41	32980.0
42	33458.0
43	34101.5
44	34745.0
45	35213.0
46	35681.0
47	35681.0
48	40383.0
49	45085.0
50	42135.0
51	39185.0
52	35934.0
53	32683.0
54	32683.0
55	30144.5
56	27606.0
57	25064.5
58	22523.0
59	20844.5
60	19166.0
61	19166.0
62	16886.5
63	14607.0
64	12764.5
65	10922.0
66	9355.5
67	7789.0
68	7789.0
69	6578.0
70	5367.0
71	4653.0
72	3939.0
73	3261.0
74	2583.0
75	2583.0
76	2020.0
77	1457.0
78	1164.0
79	871.0
80	676.5
81	482.0
82	482.0
83	350.0
84	218.0
85	173.5
86	129.0
87	89.0
88	49.0
89	49.0
90	36.5
91	24.0
92	17.5
93	11.0
94	7.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	551832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.56869466228558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.98228628719953	56.571598675555244
2	9.423292125318596	12.545925124077076
3	2.4409166254406363	4.874659006051627
4	1.0122842026615828	2.695457519937365
5	0.5115760953420376	1.7027476443674199
6	0.30424359026876424	1.2151859198135333
7	0.21522552172095888	1.0029097427281537
8	0.1617562234812404	0.8614320520517696
9	0.12118664781951706	0.726051326025903
>10	0.7437116627184378	9.527411561870167
>50	0.057403701783188286	2.5870671610966074
>100	0.023396762469999718	2.7774065517539954
>500	0.0016323322653483546	0.706485586749514
>1k	0.0010882215102322363	2.2056621279216437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3774	0.6839037968077241	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3736	0.6770176430507836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3177	0.5757186969947375	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1492	0.2703721422461909	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	754	0.13663578770350396	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	737	0.13355513997013582	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	678	0.12286348018962293	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	630	0.11416518070717174	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	588	0.10655416866002697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.8121457255106625E-4	0.0
11	0.0	0.0	0.0	1.8121457255106625E-4	0.0
12	0.0	0.0	0.0	1.8121457255106625E-4	0.0
13	0.0	0.0	0.0	3.624291451021325E-4	0.0
14	0.0	0.0	0.0	3.624291451021325E-4	0.0
15	0.0	0.0	0.0	3.624291451021325E-4	0.0
16	0.0	0.0	0.0	0.0010872874353063976	0.0
17	0.0	0.0	0.0	0.00144971658040853	0.0
18	0.0	0.0	0.0	0.00144971658040853	0.0
19	0.0	0.0	0.0	0.0016309311529595963	0.0
20	0.0	0.0	0.0	0.0021745748706127953	0.0
21	0.0	0.0	0.0	0.004167935168674524	0.0
22	0.0	0.0	0.0	0.006161295466736253	0.0
23	0.0	0.0	0.0	0.008335870337349048	0.0
24	0.0	0.0	0.0	0.012866234651125704	0.0
25	0.0	0.0	0.0	0.017577813537453427	0.0
26	0.0	1.8121457255106625E-4	0.0	0.025913683874802477	0.0
27	0.0	1.8121457255106625E-4	0.0	0.08209020136563303	0.0
28	0.0	1.8121457255106625E-4	0.0	0.14750866205656793	0.0
29	0.0	1.8121457255106625E-4	0.0	0.22851157598689456	0.0
30	0.0	1.8121457255106625E-4	0.0	0.3537308456196814	0.0
31	0.0	1.8121457255106625E-4	0.0	0.5581408834572841	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCGAA	35	8.8635273E-4	26.428572	12
GGTATCA	940	0.0	25.388296	1
TTGCGTG	60	1.3353019E-6	24.666666	7
GACCGTC	40	0.0019299214	23.125002	22
GTATACG	40	0.0019299214	23.125002	1
CGAAGAG	40	0.0019299214	23.125002	5
CGTGCGG	50	2.6996364E-4	22.199999	10
TTGGACC	95	7.1249815E-9	21.421053	4
CGACGCT	45	0.0038233243	20.555557	28
AGTGCGA	45	0.0038233243	20.555557	11
TGCGGTC	55	5.138438E-4	20.181818	12
GTGCGGT	55	5.138438E-4	20.181818	11
GCCGTCA	55	5.138438E-4	20.181818	13
GGAGTGT	65	6.8938236E-5	19.923075	6
CGCCGGA	65	6.8938236E-5	19.923075	14
TATACCG	65	6.8938236E-5	19.923075	5
TACCGAC	95	1.6721788E-7	19.473684	7
GGATACA	60	9.2285214E-4	18.5	1
TCGAACT	60	9.2285214E-4	18.5	19
TGCGTGC	70	1.21773985E-4	18.5	8
>>END_MODULE