FastQCFastQC Report
Wed 25 May 2016
SRR2088346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624706
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212481.307805843026369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT205071.2621975914411592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181191.115217153134167No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75390.4640224139013459No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59460.3659739054327368No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA56770.34941706376415177No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA56330.3467088814837884No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55440.3412309673257808No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG54160.3333526188738147No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50890.3132259005629326No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA36940.22736421235595855No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31560.19425052901878861No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA29110.17917087768494733No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC29090.17904777849038533No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.16944604131455168No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26420.16261403601636235No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25860.15916725856862718No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG21360.13146993979218394No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21070.12968500147103537No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG20440.1258073768423333No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20280.12482258328583755No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.12248369858916014No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18840.11595944127737572No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC18240.1122664654405166No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17890.11011222953568214No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.10765024564444274No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG16700.10278782745924493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.029.3031711
ATACGAC759.269639E-619.73333419
GTATTGG5900.019.127121
ATTGGAC5600.018.8303573
TTGGACC9400.018.1063824
AGTAGTG2500.017.765
GATACGA852.724239E-517.41176418
CAATGCG852.724239E-517.41176419
CGGTATA752.0679292E-417.26666621
TATACCG752.0679292E-417.2666665
GGACCCT10100.017.2178216
CATTGCG650.001580314217.07692329
CTAATAC2950.016.9322033
GTATCAA93600.016.8002152
GGGTGTA1005.8808E-616.6500027
GTCTAAC1352.220986E-816.4444451
ATCGGCT904.4477776E-516.4444453
GTAGTGT1708.54925E-1116.3235286
TACGTTA803.3836428E-416.187519
TTCTCGA1504.6748028E-916.03333310
Produced by FastQC (version 0.11.5)