##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1624706
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98836404863403	31.0	31.0	33.0	28.0	34.0
2	31.306746574457165	31.0	31.0	34.0	30.0	34.0
3	31.377600624359115	31.0	31.0	34.0	28.0	34.0
4	35.25965867055332	35.0	35.0	37.0	33.0	37.0
5	35.022436674696834	35.0	35.0	37.0	32.0	37.0
6	35.084736561568675	36.0	35.0	37.0	32.0	37.0
7	34.88665087714331	36.0	35.0	37.0	32.0	37.0
8	34.95266405121911	36.0	35.0	37.0	32.0	37.0
9	36.43941365391646	38.0	35.0	39.0	32.0	39.0
10	36.24817967066041	38.0	35.0	39.0	32.0	39.0
11	36.352204645025004	38.0	35.0	39.0	32.0	39.0
12	36.12600864402544	38.0	35.0	39.0	31.0	39.0
13	36.17839966123102	38.0	35.0	39.0	32.0	39.0
14	36.9879368944289	39.0	36.0	40.0	31.0	41.0
15	37.12914213402302	39.0	36.0	40.0	31.0	41.0
16	37.09815129629607	39.0	36.0	40.0	31.0	41.0
17	37.19204028298043	39.0	36.0	40.0	31.0	41.0
18	37.20019622011613	39.0	36.0	40.0	31.0	41.0
19	37.29856847946644	39.0	36.0	40.0	31.0	41.0
20	37.28196916857573	39.0	36.0	40.0	31.0	41.0
21	37.20918553879902	39.0	36.0	40.0	31.0	41.0
22	37.12910766624854	39.0	36.0	40.0	31.0	41.0
23	36.99960669807337	39.0	36.0	40.0	31.0	41.0
24	36.913577594961794	39.0	35.0	40.0	31.0	41.0
25	36.77201105923164	39.0	35.0	40.0	30.0	41.0
26	36.54233935247362	38.0	35.0	40.0	30.0	41.0
27	36.413943199569644	38.0	35.0	40.0	30.0	41.0
28	36.24973195150384	38.0	35.0	40.0	30.0	41.0
29	36.023391308950664	38.0	35.0	40.0	29.0	41.0
30	35.78023900939616	38.0	34.0	40.0	29.0	41.0
31	35.52526672517982	38.0	34.0	40.0	27.0	41.0
32	35.3985896525279	38.0	34.0	40.0	27.0	41.0
33	35.216677355780064	38.0	34.0	40.0	27.0	41.0
34	35.351999684866065	38.0	34.0	40.0	27.0	41.0
35	35.25497720818413	38.0	34.0	40.0	27.0	41.0
36	35.18923916080817	38.0	34.0	40.0	26.0	41.0
37	35.06094641122763	38.0	33.0	40.0	25.0	41.0
38	35.0157437714885	38.0	33.0	40.0	25.0	41.0
39	34.891666553825736	38.0	33.0	40.0	24.0	41.0
40	34.68031816217827	38.0	33.0	40.0	24.0	41.0
41	34.53377226402807	38.0	33.0	40.0	23.0	41.0
42	34.360982233093246	38.0	33.0	40.0	22.0	41.0
43	33.97341303595851	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	4.0
15	5.0
16	22.0
17	64.0
18	129.0
19	319.0
20	629.0
21	1206.0
22	2347.0
23	4096.0
24	6799.0
25	10563.0
26	16204.0
27	23315.0
28	31920.0
29	43112.0
30	55060.0
31	68260.0
32	82607.0
33	99328.0
34	119574.0
35	142654.0
36	176618.0
37	226258.0
38	266424.0
39	247186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37173494773823	22.510841961561045	14.516349419525747	22.60107367117497
2	18.324238354508445	23.83095772404361	36.120258065151475	21.724545856296462
3	19.499404815394293	25.368343564928054	33.43965000437002	21.69260161530763
4	13.292497227190642	16.592540435007933	36.65752449981719	33.45743783798423
5	11.966041856188134	38.73666989596887	35.57142030619694	13.725867941646058
6	31.293538646376636	37.545438990192686	13.742609432106487	17.41841293132419
7	24.27448412205039	34.412564488590554	22.220327862394797	19.092623526964264
8	25.962235629092277	34.68122848072205	19.744987708545423	19.61154818164025
9	23.684223484125745	14.71213868847656	23.490465351885202	38.11317247551249
10	15.838188570732182	27.626228991583705	34.622510164915994	21.91307227276812
11	31.557094021933814	22.802094655894667	24.89804309210405	20.742768230067473
12	20.971671182355454	27.308325321627418	31.752021596522695	19.96798189949443
13	31.169762406244576	21.65370226982605	27.752713413996133	19.423821909933242
14	19.91794207690499	23.736663741009142	29.057564876353016	27.287829305732853
15	21.295668262442558	29.941847940488923	27.250591799377855	21.51189199769066
16	20.776312760585608	29.09529477948626	28.10096103541195	22.027431424516188
17	20.68269582312123	26.97257226845965	27.946717744625797	24.398014163793327
18	22.54358634731453	25.50707635719939	31.35244161097454	20.596895684511537
19	21.70521928275023	24.830892481470492	32.75429523864625	20.70959299713302
20	22.88241688034635	24.30310468478605	32.75127930837948	20.063199126488115
21	21.634683444266223	25.2413051961401	33.2730352445304	19.85097611506328
22	19.88107386813368	25.658857664094302	32.61777823187703	21.842290235894986
23	21.317272171088185	25.908256632276856	33.83670645642965	18.937764740205303
24	21.148441625746443	25.781218263488903	31.188596583012558	21.8817435277521
25	20.857004282620977	26.416840954609633	32.26780722173735	20.45834754103204
26	20.487460500545946	27.2345889040848	32.118364799539115	20.159585795830136
27	19.1323230172105	27.400772816743462	31.80372325823872	21.66318090780732
28	20.08671107264945	26.54794159681813	31.853639981633602	21.511707348898817
29	20.197438798157943	26.502517994024764	30.924548810677134	22.37549439714016
30	20.616960853225137	26.81457445223936	32.79110189782028	19.77736279671522
31	21.61812660259764	26.348397802433176	32.56342993747792	19.470045657491262
32	20.969332297658777	26.500486857314492	31.198690716966638	21.331490128060093
33	20.49478490262238	26.337626622909006	32.106054880082915	21.061533594385693
34	19.46235195783114	27.23391185851471	32.90743063668135	20.39630554697281
35	18.99303627856363	29.550454051379145	31.263748641292636	20.19276102876459
36	21.71648285905265	26.809835133248722	30.91156184565084	20.56212016204778
37	20.68774289009827	27.834635927977125	30.86133737426956	20.616283807655048
38	20.480690044845037	26.084596228486877	32.213951324116486	21.2207624025516
39	20.08677262224673	25.6899402107212	33.13122497239501	21.09206219463706
40	18.535845869960475	26.419733785681842	32.67138793110876	22.373032413248918
41	20.471826902836572	25.39801047081749	32.5871265324311	21.54303609391484
42	18.90932882626149	27.727724277500055	31.809447370785854	21.553499525452605
43	19.326327347840163	28.07363301421919	30.51555173674499	22.08448790119566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	199.5
2	249.0
3	694.0
4	1139.0
5	1139.0
6	2030.0
7	2921.0
8	3236.5
9	3552.0
10	5520.0
11	7488.0
12	7488.0
13	13971.0
14	20454.0
15	32958.5
16	45463.0
17	44682.5
18	43902.0
19	43902.0
20	46431.5
21	48961.0
22	40320.0
23	31679.0
24	34554.0
25	37429.0
26	37429.0
27	40512.0
28	43595.0
29	44955.0
30	46315.0
31	50262.5
32	54210.0
33	54210.0
34	58995.0
35	63780.0
36	68809.5
37	73839.0
38	77136.5
39	80434.0
40	80434.0
41	82983.0
42	85532.0
43	88977.5
44	92423.0
45	100561.5
46	108700.0
47	108700.0
48	147290.0
49	185880.0
50	169375.0
51	152870.0
52	134651.0
53	116432.0
54	116432.0
55	99688.0
56	82944.0
57	68046.0
58	53148.0
59	47214.0
60	41280.0
61	41280.0
62	36202.0
63	31124.0
64	26785.5
65	22447.0
66	19280.5
67	16114.0
68	16114.0
69	13638.0
70	11162.0
71	9437.5
72	7713.0
73	6291.5
74	4870.0
75	4870.0
76	3971.0
77	3072.0
78	2351.0
79	1630.0
80	1254.5
81	879.0
82	879.0
83	701.5
84	524.0
85	367.0
86	210.0
87	157.5
88	105.0
89	105.0
90	82.0
91	59.0
92	39.5
93	20.0
94	13.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1624706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.895514299494536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53985788289161	35.58731656245026
2	12.150046590737942	11.152652740894759
3	4.198925088213345	5.781354792858058
4	1.8352292728431665	3.3691516533849803
5	1.0087260990822675	2.314800155235177
6	0.6255053362829099	1.7224733463469457
7	0.4129357436237587	1.3266328828379639
8	0.29367107968995654	1.078254818980672
9	0.23019230806554997	0.950831492981049
>10	1.4742169231913014	12.943366637944656
>50	0.13906649550153521	4.347985037337536
>100	0.07826778525284503	6.538789249023134
>500	0.00566762196163131	1.8324104366879774
>1k	0.006342338861825513	4.816216500795639
>5k	9.446036602718849E-4	2.5295897480678593
>10k+	4.0483014011652213E-4	3.708173944173318
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21248	1.307805843026369	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20507	1.2621975914411592	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18119	1.115217153134167	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7539	0.4640224139013459	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5946	0.3659739054327368	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5677	0.34941706376415177	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5633	0.3467088814837884	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5544	0.3412309673257808	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5416	0.3333526188738147	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5089	0.3132259005629326	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3694	0.22736421235595855	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3156	0.19425052901878861	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2911	0.17917087768494733	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2909	0.17904777849038533	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2753	0.16944604131455168	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2642	0.16261403601636235	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2586	0.15916725856862718	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2136	0.13146993979218394	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2107	0.12968500147103537	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2044	0.1258073768423333	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2028	0.12482258328583755	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1990	0.12248369858916014	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1884	0.11595944127737572	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1824	0.1122664654405166	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1789	0.11011222953568214	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1749	0.10765024564444274	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1670	0.10278782745924493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.1549597280985E-5	0.0	0.0	0.0	6.1549597280985E-5
7	1.2309919456197E-4	0.0	0.0	0.0	6.1549597280985E-5
8	1.2309919456197E-4	0.0	0.0	0.0	6.1549597280985E-5
9	1.8464879184295496E-4	0.0	0.0	0.0	6.1549597280985E-5
10	1.8464879184295496E-4	0.0	0.0	1.8464879184295496E-4	6.1549597280985E-5
11	1.8464879184295496E-4	0.0	0.0	8.001447646528049E-4	6.1549597280985E-5
12	1.8464879184295496E-4	0.0	0.0	0.0013540911401816698	1.2309919456197E-4
13	1.8464879184295496E-4	0.0	0.0	0.0014771903347436397	1.2309919456197E-4
14	2.4619838912394E-4	0.0	0.0	0.00196958711299152	1.2309919456197E-4
15	2.4619838912394E-4	0.0	0.0	0.0028928310722062946	1.2309919456197E-4
16	2.4619838912394E-4	0.0	0.0	0.00393917422598304	1.2309919456197E-4
17	2.4619838912394E-4	0.0	0.0	0.005047066977040769	1.2309919456197E-4
18	3.0774798640492495E-4	0.0	0.0	0.0056625629498506195	1.2309919456197E-4
19	5.539463755288649E-4	0.0	0.0	0.006832005298189334	1.2309919456197E-4
20	5.539463755288649E-4	0.0	0.0	0.010463431537767449	1.2309919456197E-4
21	5.539463755288649E-4	0.0	0.0	0.016679940863146933	1.2309919456197E-4
22	5.539463755288649E-4	0.0	0.0	0.02862056273565802	1.2309919456197E-4
23	5.539463755288649E-4	0.0	0.0	0.034098476893665686	1.2309919456197E-4
24	5.539463755288649E-4	0.0	0.0	0.042099924540193734	1.2309919456197E-4
25	5.539463755288649E-4	0.0	0.0	0.048254884268292234	2.4619838912394E-4
26	6.154959728098499E-4	0.0	0.0	0.059518460570712486	2.4619838912394E-4
27	6.154959728098499E-4	0.0	0.0	0.1427950656918852	2.4619838912394E-4
28	6.154959728098499E-4	0.0	0.0	0.2525995472411624	2.4619838912394E-4
29	6.154959728098499E-4	0.0	0.0	0.38400793743606537	2.4619838912394E-4
30	7.385951673718198E-4	0.0	0.0	0.5977081391956452	3.0774798640492495E-4
31	7.385951673718198E-4	0.0	0.0	0.9020708977501161	3.0774798640492495E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5360	0.0	29.303171	1
ATACGAC	75	9.269639E-6	19.733334	19
GTATTGG	590	0.0	19.12712	1
ATTGGAC	560	0.0	18.830357	3
TTGGACC	940	0.0	18.106382	4
AGTAGTG	250	0.0	17.76	5
GATACGA	85	2.724239E-5	17.411764	18
CAATGCG	85	2.724239E-5	17.411764	19
CGGTATA	75	2.0679292E-4	17.266666	21
TATACCG	75	2.0679292E-4	17.266666	5
GGACCCT	1010	0.0	17.217821	6
CATTGCG	65	0.0015803142	17.076923	29
CTAATAC	295	0.0	16.932203	3
GTATCAA	9360	0.0	16.800215	2
GGGTGTA	100	5.8808E-6	16.650002	7
GTCTAAC	135	2.220986E-8	16.444445	1
ATCGGCT	90	4.4477776E-5	16.444445	3
GTAGTGT	170	8.54925E-11	16.323528	6
TACGTTA	80	3.3836428E-4	16.1875	19
TTCTCGA	150	4.6748028E-9	16.033333	10
>>END_MODULE