FastQCFastQC Report
Wed 25 May 2016
SRR2088345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219608
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT339711.5304954748766448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT327591.475891238452916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT283261.276171287903089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115840.5218939560498971No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG95600.43070668334228385No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA92570.4170556242363516No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA92230.4155238222244648No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG90150.4061527981517457No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG88910.40056622610839393No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA88210.3974125160839211No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA55080.24815192592565893No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48910.22035422470994878No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48850.2200839067078511No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC45440.20472083358863366No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44210.19917931454563148No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA42030.18935776046941621No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA39430.17764398037851728No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG34620.15597348721035428No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG34210.15412631419602021No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG33400.15047702116770167No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32610.1469178341400824No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT32150.14484539612400027No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.14430476011980492No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31740.14299822310966623No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC29510.13295140403170289No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG27900.12569787097541546No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.11722790690968855No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA25520.114975256892208No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT24360.10974910885165308No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA23880.10758656483487175No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT23370.10528886181704157No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT23080.10398232480690285No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT22800.10272084079711373No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC22620.10190988679082073No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG22590.10177472778977188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87800.028.3399751
GTATTGG9750.023.1487181
ATTGGAC10350.021.0917853
ACCGTGT851.246377E-619.5882368
TATTGGA11950.018.7322182
ACGTTTA1501.2732926E-1118.49999826
TGCGCGA1054.802241E-717.61904712
CGCGATA953.6094498E-617.52631614
GGACCCT17250.017.159426
TTGGACC17750.016.9887334
TGGACCC17550.016.5498585
GTATCAA152300.016.422852
TATACCG1708.54925E-1116.323535
CGACGAG1251.659646E-716.27999924
CCGACCG1751.3278623E-1015.8571439
GACCCTC18150.015.4931137
CTAGACA1201.9373783E-615.4166674
CAATGCG1201.9373783E-615.41666719
TTTTTAC19600.015.3852031
CGCCGGA1601.097942E-815.03125114
Produced by FastQC (version 0.11.5)