##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2219608
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86554652893664	31.0	31.0	33.0	28.0	34.0
2	31.17076168404511	31.0	31.0	34.0	28.0	34.0
3	31.213561133317235	31.0	31.0	34.0	28.0	34.0
4	35.112183322460545	35.0	35.0	37.0	32.0	37.0
5	34.87821453157495	35.0	35.0	37.0	32.0	37.0
6	34.95086474728871	35.0	35.0	37.0	32.0	37.0
7	34.750815909836334	35.0	35.0	37.0	31.0	37.0
8	34.829125232924014	35.0	35.0	37.0	32.0	37.0
9	36.2705545303495	38.0	35.0	39.0	32.0	39.0
10	36.075483598905755	38.0	35.0	39.0	31.0	39.0
11	36.18294942169969	38.0	35.0	39.0	31.0	39.0
12	35.954251831855	37.0	35.0	39.0	30.0	39.0
13	36.02057435366966	38.0	35.0	39.0	30.0	39.0
14	36.79223133093772	38.0	36.0	40.0	31.0	41.0
15	36.91830269128603	38.0	36.0	40.0	31.0	41.0
16	36.87646422251136	38.0	36.0	40.0	31.0	41.0
17	36.99338486795867	38.0	36.0	40.0	31.0	41.0
18	37.01565997239152	38.0	36.0	40.0	31.0	41.0
19	37.12561046815474	39.0	36.0	40.0	31.0	41.0
20	37.107416715023554	39.0	36.0	40.0	31.0	41.0
21	37.0338402096226	39.0	36.0	40.0	31.0	41.0
22	36.95711449949721	39.0	36.0	40.0	31.0	41.0
23	36.82554396992622	39.0	35.0	40.0	31.0	41.0
24	36.73990317209165	38.0	35.0	40.0	30.0	41.0
25	36.59690134474195	38.0	35.0	40.0	30.0	41.0
26	36.347502802296624	38.0	35.0	40.0	30.0	41.0
27	36.21189282071429	38.0	35.0	40.0	30.0	41.0
28	36.026123081192715	38.0	34.0	40.0	29.0	41.0
29	35.80410279652984	38.0	34.0	40.0	29.0	41.0
30	35.554197858360574	38.0	34.0	40.0	27.0	41.0
31	35.255314902451246	38.0	34.0	40.0	27.0	41.0
32	35.14178809952028	37.0	33.0	40.0	27.0	41.0
33	34.94625942959297	37.0	33.0	40.0	26.0	41.0
34	35.06292462452829	37.0	33.0	40.0	26.0	41.0
35	34.95031960598448	37.0	33.0	40.0	25.0	41.0
36	34.8619968030391	38.0	33.0	40.0	25.0	41.0
37	34.72531951587848	37.0	33.0	40.0	25.0	41.0
38	34.646907922480004	38.0	33.0	40.0	24.0	41.0
39	34.506893559583496	38.0	33.0	40.0	24.0	41.0
40	34.27798647328717	38.0	33.0	40.0	23.0	41.0
41	34.103335814251885	38.0	33.0	40.0	21.0	41.0
42	33.90233500690212	37.0	33.0	40.0	20.0	41.0
43	33.514175025499995	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	7.0
15	14.0
16	26.0
17	93.0
18	229.0
19	490.0
20	1154.0
21	2137.0
22	3889.0
23	6561.0
24	11032.0
25	16829.0
26	25088.0
27	36041.0
28	49288.0
29	64710.0
30	81494.0
31	99238.0
32	117876.0
33	141433.0
34	166740.0
35	198365.0
36	242610.0
37	305557.0
38	354603.0
39	294102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.070949464950566	22.990726290408038	15.125463595373597	21.812860649267797
2	18.497320247539207	24.342181141895324	36.022802224536946	21.137696386028523
3	19.782141711509418	25.65259271006412	33.98086508969151	20.584400488734946
4	13.453321487397773	16.69893963258377	36.61669087514552	33.23104800487293
5	11.549471798623902	39.14010942472725	35.60872009832367	13.70169867832518
6	30.84846513438409	37.603802112805504	13.371189867760434	18.176542885049972
7	23.43990470389366	35.09971130037376	22.143234300831498	19.317149694901083
8	26.097130664513735	34.78028552789502	19.63616999037668	19.486413817214572
9	23.33538174308256	14.697730410054389	23.93255926271666	38.03432858414639
10	16.006429964209897	27.761839027431872	34.33272902242198	21.899001985936255
11	31.113647094441905	22.730545213389032	25.231572421797	20.924235270372066
12	20.708881928700922	27.760712702423127	31.917257461677917	19.613147907198027
13	31.501553427452055	21.595209604578827	28.294635809566376	18.608601158402745
14	19.61143589318474	24.013474451344564	29.438396329441957	26.93669332602874
15	20.93653473946751	30.083149817445243	27.94610579886178	21.034209644225466
16	20.263578073245366	29.212951115692498	28.692228537651694	21.83124227341044
17	20.228031255969523	26.976385019336746	28.27999358445275	24.515590140240977
18	22.453964844242766	25.19368284850298	32.026510987525725	20.32584131972853
19	21.44072286638001	24.428232372563084	33.660808575207874	20.470236185849032
20	22.833671531189292	23.743111396246544	33.740777650828434	19.68243942173573
21	21.530648655077833	24.78433128732641	34.461220179419065	19.22379987817669
22	19.168474793747365	25.237068887839655	33.79123701122	21.803219307192983
23	20.825118669602922	25.68048051728053	35.32389503011343	18.170505783003126
24	20.890580679110908	25.332310930578732	32.14905514847667	21.62805324183369
25	20.29065492645548	26.310501674169494	33.307007363462375	20.091836035912646
26	20.258937614209355	27.187593484975725	33.0204252282385	19.53304367257642
27	18.568458935091243	27.332979517103922	32.67743673657691	21.42112481122793
28	19.4542009219646	26.52342215382176	32.839041848830966	21.18333507538268
29	19.793990650601366	26.223324118492997	31.690640869919374	22.292044360986267
30	20.04858515557702	26.59586737838393	33.98451438271983	19.371033083319215
31	21.375260856872025	26.011755228851218	33.7390205838148	18.873963330461955
32	20.53092257731996	26.283019343956234	32.10864260716307	21.077415471560744
33	20.04164699352318	26.26026757877968	32.992041838018245	20.7060435896789
34	18.806879412941385	27.300901780854996	33.89850820505242	19.993710601151193
35	18.34954640639248	29.92118428118839	32.07341116088967	19.655858151529458
36	21.556959607282007	26.72201577936284	31.631486280460337	20.08953833289482
37	20.359405804988988	28.076173810871108	31.404103787695846	20.160316596444055
38	20.21465051486569	25.8752446377919	33.02988635831191	20.880218489030494
39	19.606750381148384	25.510090069958295	33.945903961420214	20.937255587473103
40	17.96245102738862	26.373170397655805	33.32565930560711	22.338719269348463
41	20.326336902732375	25.216524719680233	33.24866372800963	21.208474649577763
42	18.49781583054305	27.972551910067	32.40950654349777	21.120125715892176
43	19.130224796450545	28.216784224962243	30.907799935844526	21.745191042742682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	253.0
2	335.0
3	949.5
4	1564.0
5	1564.0
6	2845.0
7	4126.0
8	4724.5
9	5323.0
10	8253.0
11	11183.0
12	11183.0
13	21095.0
14	31007.0
15	51257.0
16	71507.0
17	69074.0
18	66641.0
19	66641.0
20	70491.0
21	74341.0
22	59950.0
23	45559.0
24	49399.0
25	53239.0
26	53239.0
27	56045.5
28	58852.0
29	60717.0
30	62582.0
31	66359.5
32	70137.0
33	70137.0
34	76337.5
35	82538.0
36	89925.5
37	97313.0
38	101215.5
39	105118.0
40	105118.0
41	108493.5
42	111869.0
43	116330.0
44	120791.0
45	133852.0
46	146913.0
47	146913.0
48	210684.5
49	274456.0
50	247870.0
51	221284.0
52	194323.0
53	167362.0
54	167362.0
55	141257.5
56	115153.0
57	90973.5
58	66794.0
59	57478.5
60	48163.0
61	48163.0
62	41761.0
63	35359.0
64	30194.5
65	25030.0
66	20860.5
67	16691.0
68	16691.0
69	14075.0
70	11459.0
71	9246.0
72	7033.0
73	5778.5
74	4524.0
75	4524.0
76	3541.5
77	2559.0
78	1940.5
79	1322.0
80	1022.0
81	722.0
82	722.0
83	520.5
84	319.0
85	230.0
86	141.0
87	107.5
88	74.0
89	74.0
90	58.5
91	43.0
92	25.5
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2219608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48755723804999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3169992113337	30.494013165670392
2	13.001871174074592	10.528280067241944
3	4.768342937079841	5.791756727870144
4	2.138440196543314	3.463208794307772
5	1.1670044719663466	2.3624580177898884
6	0.6981346071056401	1.6959458919031885
7	0.44219941137512797	1.2532501785077754
8	0.33408604998935465	1.0821062457102422
9	0.2494540399654388	0.9089806249227164
>10	1.5761795772212273	12.331313618969066
>50	0.17802098203812117	5.031910047335632
>100	0.11231005062498087	8.512835014097032
>500	0.007524095865703351	2.0062080492074514
>1k	0.00819789549546783	6.948525494335953
>5k	7.86099568058559E-4	2.7405536707002796
>10k+	4.4919975317631944E-4	4.848654391430573
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33971	1.5304954748766448	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32759	1.475891238452916	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28326	1.276171287903089	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11584	0.5218939560498971	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9560	0.43070668334228385	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9257	0.4170556242363516	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9223	0.4155238222244648	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9015	0.4061527981517457	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8891	0.40056622610839393	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8821	0.3974125160839211	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5508	0.24815192592565893	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4891	0.22035422470994878	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4885	0.2200839067078511	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4544	0.20472083358863366	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4421	0.19917931454563148	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4203	0.18935776046941621	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3943	0.17764398037851728	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3462	0.15597348721035428	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3421	0.15412631419602021	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3340	0.15047702116770167	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3261	0.1469178341400824	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3215	0.14484539612400027	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3203	0.14430476011980492	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3174	0.14299822310966623	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2951	0.13295140403170289	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	2790	0.12569787097541546	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2602	0.11722790690968855	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2552	0.114975256892208	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2436	0.10974910885165308	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2388	0.10758656483487175	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2337	0.10528886181704157	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	2308	0.10398232480690285	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2280	0.10272084079711373	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2262	0.10190988679082073	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	2259	0.10177472778977188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	9.010600069922256E-5	0.0	0.0	0.0	0.0
5	9.010600069922256E-5	0.0	0.0	0.0	0.0
6	1.8021200139844512E-4	0.0	0.0	0.0	0.0
7	1.8021200139844512E-4	0.0	0.0	4.505300034961128E-5	0.0
8	2.2526500174805642E-4	0.0	0.0	4.505300034961128E-5	0.0
9	2.703180020976677E-4	0.0	0.0	1.8021200139844512E-4	0.0
10	2.703180020976677E-4	0.0	0.0	5.406360041953354E-4	0.0
11	2.703180020976677E-4	0.0	0.0	0.0010362190080410595	0.0
12	2.703180020976677E-4	0.0	0.0	0.0016219080125860063	0.0
13	2.703180020976677E-4	0.0	0.0	0.0017120140132852288	0.0
14	2.703180020976677E-4	0.0	0.0	0.002072438016082119	0.0
15	3.6042400279689025E-4	0.0	0.0	0.002748233021326288	0.0
16	3.6042400279689025E-4	0.0	0.0	0.0037393990290177366	0.0
17	3.6042400279689025E-4	0.0	0.0	0.0046855120363595735	0.0
18	4.0547700314650157E-4	0.0	0.0	0.005406360041953354	0.0
19	4.0547700314650157E-4	0.0	0.0	0.006577738051043247	0.0
20	4.0547700314650157E-4	0.0	0.0	0.01054240208180904	0.0
21	4.0547700314650157E-4	0.0	0.0	0.018561836144039848	0.0
22	4.5053000349611283E-4	0.0	0.0	0.029554768229345	0.0
23	4.5053000349611283E-4	0.0	0.0	0.03581713527794097	0.0
24	4.5053000349611283E-4	0.0	0.0	0.04523321235100973	0.0
25	5.406360041953354E-4	0.0	0.0	0.05154063239995531	0.0
26	5.406360041953354E-4	0.0	0.0	0.06275882948700852	0.0
27	5.406360041953354E-4	0.0	0.0	0.14881006015476606	0.0
28	5.406360041953354E-4	0.0	0.0	0.2572526319962804	0.0
29	5.406360041953354E-4	0.0	0.0	0.38159891296120757	0.0
30	5.856890045449467E-4	0.0	0.0	0.597582996637244	0.0
31	6.757950052441692E-4	0.0	0.0	0.8879946368908384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8780	0.0	28.339975	1
GTATTGG	975	0.0	23.148718	1
ATTGGAC	1035	0.0	21.091785	3
ACCGTGT	85	1.246377E-6	19.588236	8
TATTGGA	1195	0.0	18.732218	2
ACGTTTA	150	1.2732926E-11	18.499998	26
TGCGCGA	105	4.802241E-7	17.619047	12
CGCGATA	95	3.6094498E-6	17.526316	14
GGACCCT	1725	0.0	17.15942	6
TTGGACC	1775	0.0	16.988733	4
TGGACCC	1755	0.0	16.549858	5
GTATCAA	15230	0.0	16.42285	2
TATACCG	170	8.54925E-11	16.32353	5
CGACGAG	125	1.659646E-7	16.279999	24
CCGACCG	175	1.3278623E-10	15.857143	9
GACCCTC	1815	0.0	15.493113	7
CTAGACA	120	1.9373783E-6	15.416667	4
CAATGCG	120	1.9373783E-6	15.416667	19
TTTTTAC	1960	0.0	15.385203	1
CGCCGGA	160	1.097942E-8	15.031251	14
>>END_MODULE