FastQCFastQC Report
Wed 25 May 2016
SRR2088344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372644
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371511.1015393264157143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT347621.0307046934096808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT309480.9176183433531675No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137890.40884836940987546No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG110060.32633150726848137No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA105470.3127220068290635No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA97970.2904842610130212No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG96190.28520650267268055No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA95400.28286412678005746No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG89900.26655644651495974No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57600.1707858878672045No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA52540.15578282202331464No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51260.15198758007071012No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC48770.14460464845978407No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46430.1376664717651789No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45110.13375262850155545No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA40670.12058788297845845No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG39440.11694089266462751No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG38390.1138276082503816No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37630.11157418334102265No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36020.10680048057251225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.029.8997861
GTATTGG11750.019.8382971
ATTGGAC12300.019.1016253
GTATCAA162750.017.3121342
TTGGACC20850.016.4148674
GGACCCT20350.016.3636366
TGGACCC21600.015.3310185
TATTGGA17200.013.9825582
CGAATTA2151.9826984E-1013.76744215
TTGATAC32500.013.49076934
GACCCTC22950.013.4618737
TACGTTA2102.046363E-913.21428519
TATACTG5050.013.1881195
TATACCG2305.7661964E-1012.8695655
TCTATAC3450.012.8695653
TGATACC23600.012.77754235
GATACTG11150.012.77578536
GTAATAC2900.012.758623
TGATACT10900.012.72935935
GATACCC12150.012.63785936
Produced by FastQC (version 0.11.5)