##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3372644
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93102651806713	31.0	31.0	33.0	28.0	34.0
2	31.231289160670382	31.0	31.0	34.0	28.0	34.0
3	31.300706211506462	31.0	31.0	34.0	28.0	34.0
4	35.17971241554104	35.0	35.0	37.0	33.0	37.0
5	34.926063349704265	35.0	35.0	37.0	32.0	37.0
6	34.99200152758488	36.0	35.0	37.0	32.0	37.0
7	34.816317405572605	35.0	35.0	37.0	32.0	37.0
8	34.87963686650592	36.0	35.0	37.0	32.0	37.0
9	36.3620776459063	38.0	35.0	39.0	32.0	39.0
10	36.147147164064755	38.0	35.0	39.0	31.0	39.0
11	36.25941783360473	38.0	35.0	39.0	32.0	39.0
12	36.037130808943964	38.0	35.0	39.0	30.0	39.0
13	36.103714177956526	38.0	35.0	39.0	31.0	39.0
14	36.93898555554633	39.0	36.0	40.0	31.0	41.0
15	37.05634955838802	39.0	36.0	40.0	31.0	41.0
16	37.026204366663066	39.0	36.0	40.0	31.0	41.0
17	37.08009472686711	39.0	36.0	40.0	31.0	41.0
18	37.084609582274325	39.0	36.0	40.0	31.0	41.0
19	37.17307341065348	39.0	36.0	40.0	31.0	41.0
20	37.14914203811609	39.0	36.0	40.0	31.0	41.0
21	37.079045994774425	39.0	36.0	40.0	31.0	41.0
22	36.99643247256455	39.0	36.0	40.0	31.0	41.0
23	36.87231264254395	39.0	35.0	40.0	31.0	41.0
24	36.79493655422867	39.0	35.0	40.0	30.0	41.0
25	36.658323855111895	38.0	35.0	40.0	30.0	41.0
26	36.42288424156241	38.0	35.0	40.0	30.0	41.0
27	36.289475853366085	38.0	35.0	40.0	30.0	41.0
28	36.13760242705723	38.0	35.0	40.0	30.0	41.0
29	35.93210074944169	38.0	34.0	40.0	29.0	41.0
30	35.71872216575482	38.0	34.0	40.0	28.0	41.0
31	35.49461401796336	38.0	34.0	40.0	27.0	41.0
32	35.383348791037534	38.0	34.0	40.0	27.0	41.0
33	35.212919003606665	38.0	34.0	40.0	27.0	41.0
34	35.40138004485502	38.0	34.0	40.0	27.0	41.0
35	35.32766814404366	38.0	34.0	40.0	27.0	41.0
36	35.27313822626995	38.0	34.0	40.0	26.0	41.0
37	35.17408033578403	38.0	34.0	40.0	26.0	41.0
38	35.15288361297546	38.0	34.0	40.0	26.0	41.0
39	35.054948283898334	38.0	33.0	40.0	25.0	41.0
40	34.870662305301124	38.0	33.0	40.0	24.0	41.0
41	34.75385750764089	38.0	33.0	40.0	24.0	41.0
42	34.61486210818575	38.0	33.0	40.0	23.0	41.0
43	34.2528431699284	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	8.0
15	17.0
16	44.0
17	121.0
18	284.0
19	614.0
20	1378.0
21	2799.0
22	5037.0
23	8728.0
24	14393.0
25	22407.0
26	33965.0
27	48479.0
28	67800.0
29	90548.0
30	115749.0
31	141250.0
32	172301.0
33	207562.0
34	249154.0
35	298269.0
36	367699.0
37	463771.0
38	573343.0
39	486922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32002784758782	22.622903573576103	14.029378730752489	23.02768984808358
2	18.581208096674303	23.947057560774276	35.3267644020537	22.14496994049772
3	20.008634175442175	25.436008069633203	32.76121049242079	21.79414726250384
4	13.790545340688196	16.694794944263315	36.71131610688825	32.80334360816025
5	12.250270114485845	38.644695378462714	35.628664039252286	13.476370467799152
6	31.742306629457484	37.52501005146111	13.9914559615542	16.74122735752721
7	24.8694199565682	33.83001585699528	22.337697070903424	18.962867115533093
8	26.12315441534891	34.81096730043254	19.84718814081771	19.218690143400845
9	24.1101047130975	14.511374458733266	22.737324188381578	38.64119663978766
10	15.632779504744645	27.662036076146784	34.951331952023395	21.75385246708517
11	32.27675378723636	22.53952685192982	24.574517796719725	20.60920156411409
12	20.58340577896748	26.930354938143488	31.942742845079408	20.543496437809626
13	30.909132419549767	21.449729055304978	27.46512824952767	20.176010275617585
14	20.127205836133314	23.173184006376008	28.529248862316926	28.170361295173755
15	21.74380100597632	29.766260536243966	26.55453703385237	21.93540142392734
16	21.246209205596557	28.905363269885587	27.596360600170073	22.252066924347783
17	20.92106371143827	26.95022065773915	27.884176331685172	24.244539299137415
18	22.523159871009213	25.758158880688264	30.604920056786305	21.11376119151621
19	21.959180986786627	24.956384367872804	31.804008961515063	21.28042568382551
20	23.02078132171673	24.683364149907312	31.77023723820243	20.525617290173525
21	22.105623955567204	25.443806105832696	32.13244564205412	20.318124296545975
22	20.48019298805329	25.653285671419813	31.862598009158393	22.003923331368505
23	21.624932842007635	25.89042899280209	32.72921778877343	19.75542037641684
24	21.59338489327661	25.6660649626821	30.58031028474989	22.160239859291405
25	21.433154522090085	26.297943097463	31.27228963388961	20.9966127465573
26	21.277579252361058	26.87707329916825	31.14790650895855	20.697440939512145
27	19.998908867938624	27.13725492521594	30.938189740749394	21.925646466096037
28	20.749032509805364	26.504902385190967	30.96395587556825	21.782109229435424
29	20.82434434230236	26.298358202051563	30.358140378883746	22.519157076762326
30	21.06442304613235	26.701157904599476	31.82648390995314	20.407935139315033
31	22.018363040985058	26.17753311645107	31.60819819702287	20.195905645541007
32	21.426068093756708	26.399139666089873	30.502626426032514	21.67216581412091
33	21.15361123201856	26.29865470532911	31.133615050980772	21.414119011671556
34	20.196824805701404	27.089251044581047	31.832918031075913	20.881006118641636
35	19.903938868140248	29.01145214259199	30.505324605858192	20.579284383409576
36	22.0760922291235	26.664539749822396	30.33729619847218	20.922071822581927
37	21.394786997975476	27.416976117254	30.188332951832447	20.999903932938075
38	21.164581853287807	25.904809401763128	31.37742969610786	21.553179048841205
39	20.632447421073792	25.71907974870754	32.073797293755284	21.57467553646338
40	19.462593739511195	26.300077921061337	31.578785071890188	22.65854326753728
41	21.17208338620975	25.27121154797245	31.634853841674364	21.92185122414343
42	19.69250238092132	27.48463816519028	31.001374589194707	21.821484864693694
43	20.11878514305097	27.53160428435376	30.032935584069946	22.316674988525325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	370.5
2	492.0
3	1283.0
4	2074.0
5	2074.0
6	3719.0
7	5364.0
8	5881.0
9	6398.0
10	9823.0
11	13248.0
12	13248.0
13	24679.5
14	36111.0
15	56926.0
16	77741.0
17	76674.0
18	75607.0
19	75607.0
20	81904.5
21	88202.0
22	74784.5
23	61367.0
24	68412.5
25	75458.0
26	75458.0
27	83841.5
28	92225.0
29	97448.0
30	102671.0
31	110231.5
32	117792.0
33	117792.0
34	128117.0
35	138442.0
36	149886.5
37	161331.0
38	168030.0
39	174729.0
40	174729.0
41	179597.5
42	184466.0
43	188339.5
44	192213.0
45	205364.0
46	218515.0
47	218515.0
48	285564.5
49	352614.0
50	325372.0
51	298130.0
52	269741.0
53	241352.0
54	241352.0
55	211023.0
56	180694.0
57	153692.0
58	126690.0
59	113232.5
60	99775.0
61	99775.0
62	88298.5
63	76822.0
64	66908.5
65	56995.0
66	48746.5
67	40498.0
68	40498.0
69	34382.0
70	28266.0
71	23848.0
72	19430.0
73	15849.5
74	12269.0
75	12269.0
76	9748.0
77	7227.0
78	5407.0
79	3587.0
80	2705.5
81	1824.0
82	1824.0
83	1356.0
84	888.0
85	681.0
86	474.0
87	353.0
88	232.0
89	232.0
90	182.0
91	132.0
92	84.0
93	36.0
94	23.0
95	10.0
96	10.0
97	6.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3372644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.581314330904334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90846468216547	32.9493649571016
2	13.827002505579655	12.328518899108968
3	5.068034467282569	6.778189128773445
4	2.4035908987114927	4.286209655134315
5	1.3135215130144102	2.927925772605023
6	0.7591734052592295	2.0306968926914837
7	0.5026737983339431	1.5686901026604594
8	0.3516516316269691	1.2541673539629832
9	0.2532885296083399	1.0162741999393774
>10	1.3814596994127994	11.507687075711761
>50	0.1333806870500386	4.152110247777106
>100	0.08641358075467453	7.255548412981416
>500	0.005605567652243809	1.696280005996185
>1k	0.004871505221106848	4.527657743001856
>5k	4.671306376403827E-4	1.60908150897573
>10k+	4.003976894060423E-4	4.111598043578243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37151	1.1015393264157143	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34762	1.0307046934096808	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30948	0.9176183433531675	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13789	0.40884836940987546	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11006	0.32633150726848137	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10547	0.3127220068290635	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9797	0.2904842610130212	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9619	0.28520650267268055	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9540	0.28286412678005746	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8990	0.26655644651495974	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5760	0.1707858878672045	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5254	0.15578282202331464	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5126	0.15198758007071012	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4877	0.14460464845978407	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4643	0.1376664717651789	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4511	0.13375262850155545	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4067	0.12058788297845845	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3944	0.11694089266462751	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3839	0.1138276082503816	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3763	0.11157418334102265	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3602	0.10680048057251225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9650327754723002E-5	0.0	0.0	0.0	0.0
2	2.9650327754723002E-5	0.0	0.0	0.0	0.0
3	2.9650327754723002E-5	0.0	0.0	0.0	0.0
4	2.9650327754723002E-5	0.0	0.0	2.9650327754723002E-5	0.0
5	2.9650327754723002E-5	0.0	0.0	2.9650327754723002E-5	0.0
6	5.9300655509446004E-5	0.0	0.0	2.9650327754723002E-5	0.0
7	5.9300655509446004E-5	0.0	0.0	2.9650327754723002E-5	0.0
8	5.9300655509446004E-5	0.0	0.0	5.9300655509446004E-5	0.0
9	5.9300655509446004E-5	0.0	0.0	8.8950983264169E-5	0.0
10	5.9300655509446004E-5	0.0	0.0	1.48251638773615E-4	0.0
11	5.9300655509446004E-5	0.0	0.0	3.55803933056676E-4	0.0
12	5.9300655509446004E-5	0.0	0.0	7.41258193868075E-4	0.0
13	5.9300655509446004E-5	0.0	0.0	8.8950983264169E-4	0.0
14	5.9300655509446004E-5	0.0	0.0	0.0010377614714153051	0.0
15	5.9300655509446004E-5	0.0	0.0	0.0013935654044719811	0.0
16	5.9300655509446004E-5	0.0	0.0	0.001808669993038103	0.0
17	5.9300655509446004E-5	0.0	0.0	0.002579578514660901	0.0
18	5.9300655509446004E-5	0.0	0.0	0.003024333430981746	0.0
19	5.9300655509446004E-5	0.0	0.0	0.003617339986076206	0.0
20	5.9300655509446004E-5	0.0	0.0	0.005663212601152093	0.0
21	5.9300655509446004E-5	0.0	0.0	0.011711879463115586	0.0
22	5.9300655509446004E-5	0.0	0.0	0.017908797963852693	0.0
23	5.9300655509446004E-5	0.0	0.0	0.02291970335440088	0.0
24	5.9300655509446004E-5	0.0	0.0	0.029324174149421046	0.0
25	5.9300655509446004E-5	0.0	0.0	0.03421647822895034	0.0
26	8.8950983264169E-5	0.0	0.0	0.04471269425412228	0.0
27	1.1860131101889201E-4	0.0	0.0	0.11483571939404218	0.0
28	1.77901966528338E-4	0.0	0.0	0.19417999646568093	0.0
29	2.07552294283061E-4	0.0	0.0	0.28289377710781216	0.0
30	2.07552294283061E-4	0.0	0.0	0.42764667720636984	0.0
31	2.07552294283061E-4	0.0	0.0	0.624791706447523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9380	0.0	29.899786	1
GTATTGG	1175	0.0	19.838297	1
ATTGGAC	1230	0.0	19.101625	3
GTATCAA	16275	0.0	17.312134	2
TTGGACC	2085	0.0	16.414867	4
GGACCCT	2035	0.0	16.363636	6
TGGACCC	2160	0.0	15.331018	5
TATTGGA	1720	0.0	13.982558	2
CGAATTA	215	1.9826984E-10	13.767442	15
TTGATAC	3250	0.0	13.490769	34
GACCCTC	2295	0.0	13.461873	7
TACGTTA	210	2.046363E-9	13.214285	19
TATACTG	505	0.0	13.188119	5
TATACCG	230	5.7661964E-10	12.869565	5
TCTATAC	345	0.0	12.869565	3
TGATACC	2360	0.0	12.777542	35
GATACTG	1115	0.0	12.775785	36
GTAATAC	290	0.0	12.75862	3
TGATACT	1090	0.0	12.729359	35
GATACCC	1215	0.0	12.637859	36
>>END_MODULE