FastQCFastQC Report
Wed 25 May 2016
SRR2088343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145240
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT465721.4807137134209154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT428291.3617084864748001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT384391.2221324922740395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153340.487530363342702No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG148580.4723963831058997No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA147330.46842212358993274No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA129260.410970228027114No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG127870.40655085144535874No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA124500.39583624779031173No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG122220.38858719843318795No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA82540.262428304358332No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA74740.23762892497869795No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72930.23187419719957778No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC69370.22055550609810382No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA65100.20697943559156057No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA59710.18984242855871095No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.18195749767903244No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG55830.1775063270211494No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG53150.1689855146189162No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG53010.1685403975531279No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49270.15664941308135466No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT46640.14828757105976015No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC45400.1443451056199209No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA44080.14014828757105977No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.12650862891226108No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37210.11830575727130521No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG37190.11824216911904974No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA36700.11668425938879066No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34560.1098803270974552No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA34430.10946700410779464No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC34010.10813165291042971No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA33570.10673271356080934No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG32990.10488865714540067No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG32560.10352151187190803No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT32420.10307639480611973No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA32070.10196360214164897No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT31950.10158207322811615No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT31730.10088260355330596No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA31630.10056466279202858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109900.030.670611
GTATTGG13500.023.8444461
ATTGGAC14500.021.8172423
TTGGACC23400.019.5277774
CGTATAC1551.8189894E-1219.0967733
TGGACCC23800.018.2668065
TCTATAC2550.018.1372553
TATTGGA17250.018.0173932
GGACCCT24750.017.9393946
GTATCAA190250.017.7950062
TAGGTCG2050.017.14634121
CATTGCG2050.017.14634129
GATACCT12100.016.97107536
CTGTGCG3250.016.5076929
CCCGTAT1352.2233507E-816.4444451
GACCCTC26100.016.3026837
TATACTG4350.016.1609215
CGCGATA1403.479181E-815.85714314
TATACCG2500.015.545
CCGTATA1557.223207E-915.5161292
Produced by FastQC (version 0.11.5)