##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3145240
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.001099439152497	31.0	31.0	33.0	30.0	34.0
2	31.30829793592858	31.0	31.0	34.0	30.0	34.0
3	31.373818850071853	31.0	31.0	34.0	28.0	34.0
4	35.25059073393445	35.0	35.0	37.0	33.0	37.0
5	35.028138075313805	35.0	35.0	37.0	32.0	37.0
6	35.08663631392199	36.0	35.0	37.0	32.0	37.0
7	34.89033746232402	36.0	35.0	37.0	32.0	37.0
8	34.9640567969376	36.0	35.0	37.0	32.0	37.0
9	36.43650277880225	38.0	35.0	39.0	32.0	39.0
10	36.25578970126286	38.0	35.0	39.0	32.0	39.0
11	36.352570233114164	38.0	35.0	39.0	32.0	39.0
12	36.119110465338096	38.0	35.0	39.0	31.0	39.0
13	36.19112086835981	38.0	35.0	39.0	32.0	39.0
14	36.994063092164666	39.0	36.0	40.0	31.0	41.0
15	37.128559982704026	39.0	36.0	40.0	31.0	41.0
16	37.093367119838234	39.0	36.0	40.0	31.0	41.0
17	37.19265938370363	39.0	36.0	40.0	31.0	41.0
18	37.2058443870738	39.0	36.0	40.0	31.0	41.0
19	37.305525810431	39.0	36.0	40.0	31.0	41.0
20	37.290259248896746	39.0	36.0	40.0	31.0	41.0
21	37.21476739453905	39.0	36.0	40.0	31.0	41.0
22	37.13938046063257	39.0	36.0	40.0	31.0	41.0
23	37.014088591013724	39.0	36.0	40.0	31.0	41.0
24	36.92302908522084	39.0	35.0	40.0	31.0	41.0
25	36.79108048988312	39.0	35.0	40.0	30.0	41.0
26	36.5566761201053	38.0	35.0	40.0	30.0	41.0
27	36.4284099146647	38.0	35.0	40.0	30.0	41.0
28	36.26004692805636	38.0	35.0	40.0	30.0	41.0
29	36.03437893451692	38.0	35.0	40.0	30.0	41.0
30	35.78760476148084	38.0	34.0	40.0	29.0	41.0
31	35.516143442153854	38.0	34.0	40.0	27.0	41.0
32	35.403423268176674	38.0	34.0	40.0	27.0	41.0
33	35.21538229197136	38.0	34.0	40.0	27.0	41.0
34	35.343266968498426	38.0	34.0	40.0	27.0	41.0
35	35.240331421449554	38.0	34.0	40.0	27.0	41.0
36	35.161981279647975	38.0	34.0	40.0	26.0	41.0
37	35.030862509697194	38.0	33.0	40.0	25.0	41.0
38	34.96025358955119	38.0	33.0	40.0	25.0	41.0
39	34.829033078556805	38.0	33.0	40.0	24.0	41.0
40	34.600060408744646	38.0	33.0	40.0	23.0	41.0
41	34.44130527400135	38.0	33.0	40.0	23.0	41.0
42	34.24888021263878	38.0	33.0	40.0	22.0	41.0
43	33.87251910823975	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	16.0
16	30.0
17	108.0
18	236.0
19	583.0
20	1253.0
21	2475.0
22	4541.0
23	7819.0
24	13357.0
25	20545.0
26	31567.0
27	44959.0
28	63069.0
29	84647.0
30	107445.0
31	131720.0
32	159629.0
33	192484.0
34	232460.0
35	276106.0
36	340423.0
37	437224.0
38	513952.0
39	478589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81580419936158	22.349391461382915	14.40163548727601	22.4331688519795
2	18.388485457389578	23.725979575485496	36.07324083376785	21.812294133357074
3	19.704474062392695	25.123043074614337	33.96513461611833	21.20734824687464
4	13.114802050082028	16.252146100138624	36.68257430275591	33.95047754702344
5	11.80335999796518	38.656922842136055	35.8099858834302	13.729731276468568
6	31.424915109816737	36.977559741069044	13.34114407803538	18.256381071078838
7	24.249214686319647	34.530719436354616	21.744890691966273	19.475175185359465
8	26.343522274929736	34.26034261296435	19.463315994963818	19.932819117142095
9	23.443425620938307	14.492026045707163	23.670530706718722	38.39401762663581
10	16.045389223079955	27.335561038267347	34.42564001475245	22.193409723900242
11	31.393343592221896	22.323542877491064	25.256482812122442	21.02663071816459
12	21.188494359730896	27.093131207793363	31.671859699100864	20.046514733374877
13	31.85219569889738	21.0523203316758	28.015858885172513	19.0796250842543
14	19.96702954305554	23.465554297923212	28.93531177271051	27.63210438631074
15	21.267947755974106	29.531100965268152	27.52247841182231	21.678472866935433
16	20.85278070989813	28.857161933588532	28.263884473044982	22.026172883468355
17	20.681060904732227	26.476389719067544	27.818258702038634	25.02429067416159
18	22.86858872454884	24.8292022230418	31.650048962877236	20.652160089532117
19	21.71287405730564	23.919764469484043	33.24388599916064	21.123475474049673
20	23.310621764952757	23.28159377344813	33.263026032989536	20.14475842860958
21	22.084705777619515	24.373911052892623	33.92478793351223	19.616595235975634
22	19.851140135569942	24.817851737864203	33.24064300339561	22.09036512317025
23	21.51660922536913	25.229553229642253	34.66702064071423	18.586816904274393
24	21.334365581004946	24.879405069247497	31.56417316325622	22.222056186491333
25	20.956334015846167	25.849410537828593	32.66898551461892	20.525269931706326
26	20.710216072541364	26.645756762599994	32.51519756838906	20.128829596469586
27	18.960651651384314	26.93772176368099	32.21474354898196	21.886883035952742
28	20.223639531482494	26.014040264018007	32.156242448906916	21.60607775559258
29	20.207456346733476	25.90660808078239	31.165952359756332	22.719983212727804
30	20.559766504304918	26.23211583217815	33.551366509391976	19.65675115412496
31	22.035552135926036	25.72086708804416	33.03359997965179	19.20998079637802
32	21.178383843522276	25.9305808141827	31.390418537218146	21.500616805076877
33	20.707418193842123	25.975346873370555	32.2123907873485	21.104844145438822
34	19.477082829927127	27.134081977845888	33.09257799086874	20.29625720135824
35	18.95241698566723	30.074588902595668	31.126845646119218	19.846148465617887
36	22.10346428253488	26.757131411275452	30.953726901603694	20.185677404585977
37	21.057153031247218	28.10748941257265	30.549210871030507	20.28614668514962
38	20.858376467296612	26.00774503694472	32.05132199768539	21.08255649807328
39	20.317463850135443	25.63591331663084	32.97643423077412	21.070188602459588
40	18.506632244280247	26.655708308427972	32.25340514555328	22.5842543017385
41	21.185155981737484	25.442986862687743	32.12797751522936	21.243879640345412
42	19.16963411377192	28.3024188933118	31.35484096603121	21.173106026885073
43	19.777950172323894	28.47652325418728	29.775502028462057	21.97002454502677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	289.0
2	386.0
3	1259.0
4	2132.0
5	2132.0
6	3632.5
7	5133.0
8	5860.0
9	6587.0
10	10254.0
11	13921.0
12	13921.0
13	26540.5
14	39160.0
15	65284.0
16	91408.0
17	89082.5
18	86757.0
19	86757.0
20	92255.5
21	97754.0
22	78912.0
23	60070.0
24	65002.5
25	69935.0
26	69935.0
27	74056.0
28	78177.0
29	81446.5
30	84716.0
31	90230.5
32	95745.0
33	95745.0
34	105323.0
35	114901.0
36	125672.5
37	136444.0
38	141751.5
39	147059.0
40	147059.0
41	151989.0
42	156919.0
43	163911.0
44	170903.0
45	187884.0
46	204865.0
47	204865.0
48	294969.0
49	385073.0
50	351012.0
51	316951.0
52	281776.5
53	246602.0
54	246602.0
55	207757.5
56	168913.0
57	136505.5
58	104098.0
59	90350.0
60	76602.0
61	76602.0
62	66402.0
63	56202.0
64	48808.5
65	41415.0
66	35485.5
67	29556.0
68	29556.0
69	25360.0
70	21164.0
71	18004.0
72	14844.0
73	12155.0
74	9466.0
75	9466.0
76	7446.5
77	5427.0
78	4131.5
79	2836.0
80	2121.5
81	1407.0
82	1407.0
83	1117.0
84	827.0
85	585.5
86	344.0
87	270.5
88	197.0
89	197.0
90	155.5
91	114.0
92	68.5
93	23.0
94	18.0
95	13.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3145240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.32518825453584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9803038804952	26.866783929581807
2	14.082619075375943	10.512728162106491
3	5.385461490791196	6.03040091944004
4	2.7063735681293632	4.040636116701135
5	1.493481942273491	2.787224732505394
6	0.9426410616649258	2.1110553049859324
7	0.6245597845051613	1.6318268073007236
8	0.41085044662321757	1.2268056211737381
9	0.31236833891091437	1.049328634914588
>10	1.7363700142831882	12.145059712340796
>50	0.17594165601881204	4.589804833046567
>100	0.13044033954667983	9.22732264791387
>500	0.009594013616407514	2.465858942293143
>1k	0.007281171048162102	6.122554811384995
>5k	8.566083586073061E-4	2.0578186453439047
>10k+	8.566083586073061E-4	7.134790178966957
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46572	1.4807137134209154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42829	1.3617084864748001	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38439	1.2221324922740395	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15334	0.487530363342702	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14858	0.4723963831058997	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14733	0.46842212358993274	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12926	0.410970228027114	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12787	0.40655085144535874	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12450	0.39583624779031173	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	12222	0.38858719843318795	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8254	0.262428304358332	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7474	0.23762892497869795	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7293	0.23187419719957778	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6937	0.22055550609810382	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6510	0.20697943559156057	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5971	0.18984242855871095	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5723	0.18195749767903244	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5583	0.1775063270211494	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5315	0.1689855146189162	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5301	0.1685403975531279	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4927	0.15664941308135466	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4664	0.14828757105976015	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4540	0.1443451056199209	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4408	0.14014828757105977	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3979	0.12650862891226108	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3721	0.11830575727130521	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3719	0.11824216911904974	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3670	0.11668425938879066	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3456	0.1098803270974552	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3443	0.10946700410779464	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3401	0.10813165291042971	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3357	0.10673271356080934	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3299	0.10488865714540067	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3256	0.10352151187190803	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3242	0.10307639480611973	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3207	0.10196360214164897	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	3195	0.10158207322811615	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3173	0.10088260355330596	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	3163	0.10056466279202858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.358815225547175E-5	0.0
11	0.0	0.0	0.0	1.271763045109435E-4	0.0
12	0.0	0.0	0.0	2.8614668514962294E-4	0.0
13	0.0	0.0	0.0	2.8614668514962294E-4	0.0
14	6.358815225547175E-5	0.0	0.0	4.451170657883023E-4	0.0
15	6.358815225547175E-5	0.0	0.0	6.676755986824535E-4	0.0
16	6.358815225547175E-5	0.0	0.0	0.001017410436087548	0.0
17	6.358815225547175E-5	0.0	0.0	0.001589703806386794	0.0
18	6.358815225547175E-5	0.0	0.0	0.002034820872175096	0.0
19	6.358815225547175E-5	0.0	0.0	0.0025753201663466065	0.0
20	6.358815225547175E-5	0.0	0.0	0.00432399435337208	0.0
21	6.358815225547175E-5	0.0	0.0	0.008266459793211329	0.0
22	6.358815225547175E-5	0.0	0.0	0.01389401126782058	0.0
23	6.358815225547175E-5	0.0	0.0	0.017709300403148887	0.0
24	6.358815225547175E-5	0.0	0.0	0.02222405921328738	0.0
25	6.358815225547175E-5	0.0	0.0	0.026516259490531723	0.0
26	6.358815225547175E-5	0.0	0.0	0.03510066004502041	0.0
27	9.538222838320764E-5	0.0	0.0	0.09363355419618216	0.0
28	9.538222838320764E-5	0.0	0.0	0.17000292505500375	0.0
29	9.538222838320764E-5	0.0	0.0	0.2606478360951787	0.0
30	9.538222838320764E-5	0.0	0.0	0.41065228726583664	0.0
31	9.538222838320764E-5	0.0	0.0	0.6023387722399562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10990	0.0	30.67061	1
GTATTGG	1350	0.0	23.844446	1
ATTGGAC	1450	0.0	21.817242	3
TTGGACC	2340	0.0	19.527777	4
CGTATAC	155	1.8189894E-12	19.096773	3
TGGACCC	2380	0.0	18.266806	5
TCTATAC	255	0.0	18.137255	3
TATTGGA	1725	0.0	18.017393	2
GGACCCT	2475	0.0	17.939394	6
GTATCAA	19025	0.0	17.795006	2
TAGGTCG	205	0.0	17.146341	21
CATTGCG	205	0.0	17.146341	29
GATACCT	1210	0.0	16.971075	36
CTGTGCG	325	0.0	16.507692	9
CCCGTAT	135	2.2233507E-8	16.444445	1
GACCCTC	2610	0.0	16.302683	7
TATACTG	435	0.0	16.160921	5
CGCGATA	140	3.479181E-8	15.857143	14
TATACCG	250	0.0	15.54	5
CCGTATA	155	7.223207E-9	15.516129	2
>>END_MODULE