FastQCFastQC Report
Wed 25 May 2016
SRR2088342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971671
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT410971.3829592845237577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT389651.3112151378803374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT355781.1972388598872488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141300.4754900525663843No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA137180.4616257990874495No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG133130.44799710331325376No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG123400.41525458235450696No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA119950.4036449526209328No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA118370.39832807871396264No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG117900.3967464769821424No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA76940.25891156860904185No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA70840.23838439719605567No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA65810.22145789355551135No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC61590.20725712906980615No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA54870.18464358941484438No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53200.17902385560178094No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51370.1728657041778851No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG50870.17118314914403376No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48490.16317418718290147No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG47600.1601792392226461No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC43120.14510354611933823No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41570.13988762551439912No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC40510.13632060884263433No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39490.13288819657357764No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.11976426730953729No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG34630.11653376164454275No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA32650.10987084371049151No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT32270.1085921018847645No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC32060.10788542877054694No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30740.10344348348117945No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT30600.10297236807170107No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA30230.1017272773466511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.030.406671
GTATTGG12500.020.721
ATTGGAC12250.020.2367343
TTGGACC19950.018.9172944
TATACCG2500.018.55
CAATGCG1700.018.49999819
GTATCAA178200.017.2542082
GGACCCT21800.016.8876156
CGACGAG2100.016.73809424
TATTGGA15400.016.6980512
TGGACCC22050.016.5283455
TTTTTAC21900.015.4589031
GTATACG2650.015.3584891
TAACGTG855.3677073E-415.2352935
GGTATCG1353.9780025E-715.07407535
CGCTTAC750.00410644214.827
CGCCGGA2650.014.66037714
ATCGTCA2301.8189894E-1214.47826110
TAATACT5500.014.4636364
GACCCTC24300.014.3888887
Produced by FastQC (version 0.11.5)