##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2971671
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0601520154822	31.0	31.0	33.0	30.0	34.0
2	31.366093016353425	31.0	31.0	34.0	30.0	34.0
3	31.431149343248293	31.0	31.0	34.0	28.0	34.0
4	35.30281245804128	37.0	35.0	37.0	33.0	37.0
5	35.08394502621589	35.0	35.0	37.0	32.0	37.0
6	35.14715794581567	37.0	35.0	37.0	32.0	37.0
7	34.96408451675842	36.0	35.0	37.0	32.0	37.0
8	35.03914666192859	37.0	35.0	37.0	32.0	37.0
9	36.53219518580624	38.0	35.0	39.0	32.0	39.0
10	36.34994553569356	38.0	35.0	39.0	32.0	39.0
11	36.44280810358886	38.0	35.0	39.0	32.0	39.0
12	36.21159542896909	38.0	35.0	39.0	32.0	39.0
13	36.28734237403804	38.0	35.0	39.0	32.0	39.0
14	37.13385936733911	39.0	36.0	41.0	31.0	41.0
15	37.25453389692197	39.0	36.0	41.0	32.0	41.0
16	37.22097399072778	39.0	36.0	40.0	32.0	41.0
17	37.314612216493686	39.0	36.0	41.0	32.0	41.0
18	37.323698350187485	39.0	36.0	40.0	32.0	41.0
19	37.41749406310456	39.0	36.0	41.0	32.0	41.0
20	37.39762510722082	39.0	36.0	41.0	32.0	41.0
21	37.334186388735496	39.0	36.0	41.0	31.0	41.0
22	37.24988600689645	39.0	36.0	40.0	31.0	41.0
23	37.14448100075681	39.0	36.0	40.0	31.0	41.0
24	37.05753597891557	39.0	36.0	40.0	31.0	41.0
25	36.918348969317265	39.0	35.0	40.0	31.0	41.0
26	36.699357028419364	38.0	35.0	40.0	30.0	41.0
27	36.5732838527549	38.0	35.0	40.0	30.0	41.0
28	36.419907183534114	38.0	35.0	40.0	30.0	41.0
29	36.19543617042398	38.0	35.0	40.0	30.0	41.0
30	35.96386241949395	38.0	35.0	40.0	29.0	41.0
31	35.70150632421961	38.0	34.0	40.0	29.0	41.0
32	35.59859722021718	38.0	34.0	40.0	29.0	41.0
33	35.41304134946298	38.0	34.0	40.0	27.0	41.0
34	35.55514153484689	38.0	34.0	40.0	28.0	41.0
35	35.437023479382475	38.0	34.0	40.0	27.0	41.0
36	35.36257412075563	38.0	34.0	40.0	27.0	41.0
37	35.2456732256027	38.0	34.0	40.0	26.0	41.0
38	35.189233936058194	38.0	34.0	40.0	26.0	41.0
39	35.06209402050227	38.0	34.0	40.0	25.0	41.0
40	34.83907168727628	38.0	33.0	40.0	24.0	41.0
41	34.69023253247079	38.0	33.0	40.0	23.0	41.0
42	34.50860038005553	38.0	33.0	40.0	23.0	41.0
43	34.14497331635972	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	2.0
15	8.0
16	42.0
17	73.0
18	206.0
19	499.0
20	1020.0
21	2090.0
22	3827.0
23	6801.0
24	11469.0
25	17809.0
26	27082.0
27	39517.0
28	55135.0
29	74721.0
30	95964.0
31	118100.0
32	144318.0
33	176743.0
34	215201.0
35	258738.0
36	322458.0
37	415740.0
38	501527.0
39	482578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.707904744502336	22.348133423922096	14.460450029629795	22.483511801945774
2	18.258851669649836	23.82047003184404	36.24852818498414	21.672150113521987
3	19.651872633276028	25.17701320233633	33.748722520090546	21.422391644297097
4	13.18258313252039	16.244463132022354	36.63416979874286	33.938783936714394
5	11.859085342892937	38.70593346302468	35.627833632996385	13.807147561086003
6	31.469600773436895	37.09044507282266	13.408987737875425	18.030966415865013
7	24.305887159110142	34.47622566562718	21.85218350214408	19.365703673118592
8	26.16652381774429	34.48443653419238	19.519926667521403	19.829112980541925
9	23.59117143183078	14.555278831337654	23.550588204414282	38.302961532417285
10	15.899236490176738	27.418950482741867	34.511761227942124	22.170051799139273
11	31.58169258979207	22.424285864754207	25.172436652644254	20.821584892809465
12	21.10415318519446	27.13008943453027	31.772527981731493	19.993229398543782
13	31.730699663589945	21.357714228795853	27.85597732723441	19.055608780379792
14	20.04138412361261	23.590498409817236	28.68268391756692	27.685433549003235
15	21.061281682931927	29.87060142256663	27.34431234144022	21.723804553061225
16	20.79123159999879	29.161875591207775	28.03624627356124	22.010646535232198
17	20.708348939031275	26.62636610849586	27.750851288719375	24.914433663753492
18	22.896713667158984	25.155947613312513	31.446246909567044	20.501091809961466
19	21.78733783113945	24.255511461396633	32.766817053435595	21.190333654028322
20	23.27347139033897	23.591911756045672	32.92191497645601	20.21270187715935
21	22.063647018798516	24.71787758469898	33.48028768998991	19.738187706512598
22	19.768608301524633	25.02689564221611	33.063720714709	22.14077534155026
23	21.485453806965847	25.497674540687715	34.41282699195167	18.604044660394774
24	21.33429306272464	25.062161995725635	31.258911232098036	22.344633709451685
25	20.990109605000015	25.992884138250837	32.38649231358384	20.63051394316531
26	20.772050472612882	26.850617043407567	32.21184310107007	20.16548938290948
27	18.907813146206294	27.117268365172322	32.029420484299905	21.945498004321472
28	20.16485001199662	26.18096013993474	31.866078041613626	21.78811180645502
29	20.203851637681293	26.07307471116419	30.96698793372483	22.756085717429688
30	20.640205460160292	26.34194700557363	33.14643512017313	19.871412414092944
31	22.067012128866217	25.830214717578087	32.71798930635323	19.384783847202467
32	21.237512497177512	26.085996733824167	31.193291585777832	21.483199183220485
33	20.65709831270016	25.965256584594997	32.14292564688352	21.23471945582132
34	19.44323580907846	27.11447532381613	32.96963223721603	20.47265662988938
35	18.943483312923938	29.789266712230255	31.212809224170506	20.054440750675294
36	22.08844787999748	26.57491357556069	30.819360555054715	20.517277989387118
37	21.033452222672025	27.812903918367816	30.692260347797585	20.461383511162577
38	20.7449276854672	25.77455579705829	32.20417065011571	21.276345867358803
39	20.27287004516987	25.344898543614015	33.18701834758962	21.195213063626493
40	18.506961234941553	26.228071680882575	32.48095095318425	22.78401613099162
41	20.928797299566472	25.141141129014617	32.4111585703801	21.518903001038808
42	19.018323360829648	27.965343404434744	31.691025015891732	21.325308218843876
43	19.556236205151915	28.291456221095807	30.241200994322725	21.911106579429553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	285.0
2	394.0
3	1192.0
4	1990.0
5	1990.0
6	3461.5
7	4933.0
8	5529.0
9	6125.0
10	9694.0
11	13263.0
12	13263.0
13	24947.5
14	36632.0
15	60433.0
16	84234.0
17	82580.0
18	80926.0
19	80926.0
20	84544.5
21	88163.0
22	71429.0
23	54695.0
24	59525.0
25	64355.0
26	64355.0
27	68535.5
28	72716.0
29	75730.0
30	78744.0
31	85063.0
32	91382.0
33	91382.0
34	100298.5
35	109215.0
36	119623.5
37	130032.0
38	136657.5
39	143283.0
40	143283.0
41	147768.0
42	152253.0
43	159017.5
44	165782.0
45	182888.5
46	199995.0
47	199995.0
48	283240.5
49	366486.0
50	333170.5
51	299855.0
52	265233.0
53	230611.0
54	230611.0
55	195250.0
56	159889.0
57	128571.5
58	97254.0
59	83911.5
60	70569.0
61	70569.0
62	61632.0
63	52695.0
64	45051.0
65	37407.0
66	32107.0
67	26807.0
68	26807.0
69	22525.0
70	18243.0
71	15779.5
72	13316.0
73	10928.0
74	8540.0
75	8540.0
76	6889.0
77	5238.0
78	3991.5
79	2745.0
80	2088.5
81	1432.0
82	1432.0
83	1054.0
84	676.0
85	515.0
86	354.0
87	264.0
88	174.0
89	174.0
90	120.5
91	67.0
92	42.5
93	18.0
94	11.0
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2971671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.08603528883622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14746686111003	28.590444710223252
2	13.849250650577751	10.8262459930484
3	5.136026492528331	6.022407381940802
4	2.488439757993315	3.890529767802791
5	1.3289859915639883	2.597239668231952
6	0.7992411689083884	1.8743501119346382
7	0.5336776831543534	1.4601541329644787
8	0.38782359659845594	1.2126789425992506
9	0.2924812958312507	1.0288740825166331
>10	1.7312553502535217	12.948516795251258
>50	0.1761438049540579	4.7684989231571295
>100	0.11347072784977892	8.297449406198636
>500	0.0070036599606853435	1.8888763682990868
>1k	0.007176589836256729	6.032873280224564
>5k	6.917195022898053E-4	1.6407482207250148
>10k+	8.646493778622567E-4	6.920112214882206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41097	1.3829592845237577	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38965	1.3112151378803374	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35578	1.1972388598872488	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14130	0.4754900525663843	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13718	0.4616257990874495	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13313	0.44799710331325376	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12340	0.41525458235450696	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11995	0.4036449526209328	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11837	0.39832807871396264	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11790	0.3967464769821424	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7694	0.25891156860904185	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7084	0.23838439719605567	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6581	0.22145789355551135	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6159	0.20725712906980615	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5487	0.18464358941484438	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5320	0.17902385560178094	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5137	0.1728657041778851	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5087	0.17118314914403376	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4849	0.16317418718290147	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4760	0.1601792392226461	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4312	0.14510354611933823	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4157	0.13988762551439912	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4051	0.13632060884263433	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3949	0.13288819657357764	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3559	0.11976426730953729	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3463	0.11653376164454275	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3265	0.10987084371049151	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3227	0.1085921018847645	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3206	0.10788542877054694	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3074	0.10344348348117945	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3060	0.10297236807170107	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3023	0.1017272773466511	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.3651100677026496E-5	0.0	0.0	0.0	0.0
6	3.3651100677026496E-5	0.0	0.0	0.0	0.0
7	3.3651100677026496E-5	0.0	0.0	0.0	0.0
8	2.6920880541621197E-4	0.0	0.0	0.0	0.0
9	2.6920880541621197E-4	0.0	0.0	0.0	0.0
10	2.6920880541621197E-4	0.0	0.0	3.3651100677026496E-5	3.3651100677026496E-5
11	2.6920880541621197E-4	0.0	0.0	2.3555770473918547E-4	3.3651100677026496E-5
12	2.6920880541621197E-4	0.0	0.0	5.720687115094504E-4	3.3651100677026496E-5
13	3.0285990609323847E-4	0.0	0.0	6.730220135405299E-4	3.3651100677026496E-5
14	3.0285990609323847E-4	0.0	0.0	8.412775169256624E-4	3.3651100677026496E-5
15	3.0285990609323847E-4	0.0	0.0	0.0013460440270810599	3.3651100677026496E-5
16	3.0285990609323847E-4	0.0	0.0	0.0022209726446837485	3.3651100677026496E-5
17	3.0285990609323847E-4	0.0	0.0	0.0032305056649945436	3.3651100677026496E-5
18	3.0285990609323847E-4	0.0	0.0	0.0038025743765039938	3.3651100677026496E-5
19	3.0285990609323847E-4	0.0	0.0	0.005047665101553974	3.3651100677026496E-5
20	3.0285990609323847E-4	0.0	0.0	0.008177217464517439	3.3651100677026496E-5
21	3.0285990609323847E-4	0.0	0.0	0.015344901908724081	3.3651100677026496E-5
22	3.0285990609323847E-4	0.0	0.0	0.024430699091521235	3.3651100677026496E-5
23	3.0285990609323847E-4	0.0	0.0	0.031800290139790036	3.3651100677026496E-5
24	3.0285990609323847E-4	0.0	0.0	0.039741949899568293	3.3651100677026496E-5
25	3.0285990609323847E-4	0.0	0.0	0.045328032611954686	3.3651100677026496E-5
26	3.0285990609323847E-4	0.0	0.0	0.05589447822454101	3.3651100677026496E-5
27	3.0285990609323847E-4	0.0	0.0	0.13887809249408833	3.3651100677026496E-5
28	3.0285990609323847E-4	0.0	0.0	0.237509468578453	3.3651100677026496E-5
29	3.0285990609323847E-4	0.0	0.0	0.3473803122889445	3.3651100677026496E-5
30	3.0285990609323847E-4	0.0	0.0	0.5369706135033118	3.3651100677026496E-5
31	3.0285990609323847E-4	0.0	0.0	0.7918777011317875	3.3651100677026496E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10045	0.0	30.40667	1
GTATTGG	1250	0.0	20.72	1
ATTGGAC	1225	0.0	20.236734	3
TTGGACC	1995	0.0	18.917294	4
TATACCG	250	0.0	18.5	5
CAATGCG	170	0.0	18.499998	19
GTATCAA	17820	0.0	17.254208	2
GGACCCT	2180	0.0	16.887615	6
CGACGAG	210	0.0	16.738094	24
TATTGGA	1540	0.0	16.698051	2
TGGACCC	2205	0.0	16.528345	5
TTTTTAC	2190	0.0	15.458903	1
GTATACG	265	0.0	15.358489	1
TAACGTG	85	5.3677073E-4	15.235293	5
GGTATCG	135	3.9780025E-7	15.074075	35
CGCTTAC	75	0.004106442	14.8	27
CGCCGGA	265	0.0	14.660377	14
ATCGTCA	230	1.8189894E-12	14.478261	10
TAATACT	550	0.0	14.463636	4
GACCCTC	2430	0.0	14.388888	7
>>END_MODULE