FastQCFastQC Report
Wed 25 May 2016
SRR2088341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056189
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT407571.3335889894244106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT388041.2696858734849186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT345391.1301329858853624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145510.4761158423121083No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA117540.38459663325795623No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG109360.3578312728695771No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA108310.35439562147498077No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG106510.3485059333699585No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG104240.34107838225973586No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA102310.3347633277915731No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA63470.20767694668098077No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA62190.2034887240285205No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57970.1896806774711904No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC54120.17708328902433718No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.17171712875087242No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50960.1667436143510758No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48470.15859621247246164No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG44610.145966103536136No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42810.1400764154311137No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40860.13369591998400623No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38720.12669373523692415No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38270.1252213132106686No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.12518859272119623No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36650.11992059391614852No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35020.11458715413215609No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.10483644826939695No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG30930.10120447393796653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97650.029.4598061
CTCGCGA450.003827237820.55555714
ACGTTTA1450.020.41379426
GTATTGG9500.020.0578941
ATTGGAC10050.018.9601973
TTGGACC17650.017.3994334
GTATCAA169200.017.1114062
GGACCCT18550.016.2560656
ACCGGAT700.00259381415.8571428
GTATAAC2003.6379788E-1215.7251
TCACGTT2003.6379788E-1215.72524
TGGACCC20100.015.6467665
CCGACCG2550.015.2352949
TATACCG2400.014.6458335
GACCCTC21300.014.5915497
CGTATAC1152.2121063E-514.4782613
TACGTTA2059.276846E-1114.43902519
CGTGTCT3400.014.14705935
CGACCGC2500.014.05999910
TTTTTAC20550.013.8637471
Produced by FastQC (version 0.11.5)