##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088341_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3056189
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.026621390234702	31.0	31.0	33.0	30.0	34.0
2	31.33855301488226	31.0	31.0	34.0	30.0	34.0
3	31.407004933268198	31.0	31.0	34.0	28.0	34.0
4	35.28416959814985	37.0	35.0	37.0	33.0	37.0
5	35.050263579902946	35.0	35.0	37.0	32.0	37.0
6	35.110150255759706	37.0	35.0	37.0	32.0	37.0
7	34.91892909764416	36.0	35.0	37.0	32.0	37.0
8	34.993683636712255	36.0	35.0	37.0	32.0	37.0
9	36.48039306469593	38.0	35.0	39.0	32.0	39.0
10	36.29279995445308	38.0	35.0	39.0	32.0	39.0
11	36.394406236001764	38.0	35.0	39.0	32.0	39.0
12	36.16175832057507	38.0	35.0	39.0	31.0	39.0
13	36.233057248749994	38.0	35.0	39.0	32.0	39.0
14	37.06567362162484	39.0	36.0	40.0	31.0	41.0
15	37.19950598604995	39.0	36.0	40.0	32.0	41.0
16	37.166717110754604	39.0	36.0	40.0	31.0	41.0
17	37.25129499517209	39.0	36.0	40.0	32.0	41.0
18	37.26132709724431	39.0	36.0	40.0	32.0	41.0
19	37.358213120981716	39.0	36.0	41.0	32.0	41.0
20	37.34426241309029	39.0	36.0	41.0	31.0	41.0
21	37.26927719457141	39.0	36.0	40.0	31.0	41.0
22	37.193265207092885	39.0	36.0	40.0	31.0	41.0
23	37.065924587779094	39.0	36.0	40.0	31.0	41.0
24	36.98232111953809	39.0	36.0	40.0	31.0	41.0
25	36.84655988225859	39.0	35.0	40.0	31.0	41.0
26	36.62290388454379	38.0	35.0	40.0	30.0	41.0
27	36.49701769098704	38.0	35.0	40.0	30.0	41.0
28	36.34066643129728	38.0	35.0	40.0	30.0	41.0
29	36.11395303104618	38.0	35.0	40.0	30.0	41.0
30	35.87208120963723	38.0	34.0	40.0	29.0	41.0
31	35.621503774799265	38.0	34.0	40.0	28.0	41.0
32	35.500332930980385	38.0	34.0	40.0	28.0	41.0
33	35.32050733773337	38.0	34.0	40.0	27.0	41.0
34	35.45781723577959	38.0	34.0	40.0	27.0	41.0
35	35.36102021177355	38.0	34.0	40.0	27.0	41.0
36	35.294026645603395	38.0	34.0	40.0	27.0	41.0
37	35.16829783760101	38.0	34.0	40.0	26.0	41.0
38	35.12697087778275	38.0	34.0	40.0	25.0	41.0
39	35.00272169031431	38.0	34.0	40.0	25.0	41.0
40	34.794352705280986	38.0	33.0	40.0	24.0	41.0
41	34.655860615950125	38.0	33.0	40.0	23.0	41.0
42	34.47778753211925	38.0	33.0	40.0	23.0	41.0
43	34.10610076798261	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	17.0
16	41.0
17	80.0
18	236.0
19	544.0
20	1123.0
21	2276.0
22	4194.0
23	7358.0
24	11859.0
25	19162.0
26	29168.0
27	42110.0
28	58416.0
29	78551.0
30	101349.0
31	124352.0
32	151078.0
33	184931.0
34	222106.0
35	267973.0
36	330357.0
37	425921.0
38	510690.0
39	482293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32960657865073	22.629883164948243	14.584242008593055	22.456268247807973
2	18.22701410154935	23.84535118737748	36.250703081517536	21.676931629555632
3	19.658470074985544	25.311981686996454	33.44721154352692	21.58233669449108
4	13.412554001077812	16.505589150409218	36.499509683465256	33.58234716504771
5	11.99133954084646	38.72136180059545	35.58153635131859	13.705762307239505
6	31.41824671183621	37.33391488549955	13.702850183676468	17.544988218987765
7	24.220982406520015	34.368326042662936	22.192279338745085	19.218412212071964
8	25.87601748452075	34.879027442347315	19.722896718756594	19.522058354375336
9	23.64326944439627	14.708907073482694	23.466775124182437	38.18104835793859
10	15.823236062952914	27.67332125074725	34.64566491143054	21.857777774869287
11	31.496252358738285	22.746269946001377	24.94057141099585	20.816906284264487
12	20.789257470660356	27.316078946688176	31.797640787268065	20.097022795383403
13	31.28118058143655	21.453221643033203	27.90396798103782	19.361629794492423
14	19.885092185071017	23.656194037737848	28.961363318826162	27.497350458364977
15	21.20075034626458	30.000566064467872	27.26941952870061	21.529264060566934
16	20.670580255344156	29.088089774552557	28.226461125277265	22.014868844826026
17	20.6466288570504	26.91243898855732	28.00605590819154	24.434876246200744
18	22.653932724710415	25.39240865011948	31.414189371141642	20.539469254028464
19	21.675262884592545	24.642422310923834	32.89292645186538	20.789388352618243
20	22.9040808667265	24.1240970371924	32.80716604895836	20.16465604712274
21	21.676408101724075	25.065760003717045	33.43886781871148	19.818964075847404
22	19.81261630088977	25.400523331508623	32.9967812854506	21.790079082151006
23	21.400541654982725	25.720169793163972	34.015730048108935	18.86355850374437
24	21.127587331804413	25.40886705632407	31.44445582390356	22.019089787967953
25	20.858592187852256	26.27569826342546	32.361709305281835	20.504000243440444
26	20.549841649191197	26.986060089870094	32.283703658379764	20.180394602558938
27	19.144267582927625	27.134905596479797	32.04579297942634	21.675033841166236
28	20.21458751405754	26.353867512774897	31.942396232693724	21.489148740473837
29	20.149506460497044	26.291960346693216	31.120621139595748	22.43791205321399
30	20.588124621873842	26.60568439975407	33.03139956331235	19.774791415059738
31	21.76076152358378	26.026597177072492	32.60923980814014	19.603401491203588
32	21.018693542840445	26.255542441910496	31.371194647974974	21.354569367274078
33	20.561621025401244	26.13673434463641	32.1615580711795	21.14008655878285
34	19.54345755449025	26.913060677857292	33.053289570769344	20.49019219688311
35	19.103694175981918	29.409928509002555	31.36144394211222	20.12493337290331
36	21.698592593586326	26.610396150238092	31.144376214952672	20.546635041222906
37	20.806501168612282	27.575683310161775	31.016079175731605	20.60173634549434
38	20.58707756621073	25.79451074524514	32.382159611202056	21.236252077342073
39	20.145514560781418	25.456442648016857	33.16211791875437	21.235924872447352
40	18.537531546641915	26.23738257025334	32.71168766067805	22.51339822242669
41	20.695218783916832	25.168665943107577	32.52822387620661	21.607891396768984
42	18.990415841428653	27.70309689616709	31.81599698186205	21.490490280542204
43	19.510998828933683	27.943003524978334	30.50586858338931	22.040129062698675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	419.0
1	456.0
2	493.0
3	1309.5
4	2126.0
5	2126.0
6	3708.0
7	5290.0
8	5965.5
9	6641.0
10	10431.5
11	14222.0
12	14222.0
13	26421.0
14	38620.0
15	61897.0
16	85174.0
17	83077.0
18	80980.0
19	80980.0
20	86466.0
21	91952.0
22	75812.0
23	59672.0
24	65961.0
25	72250.0
26	72250.0
27	77387.5
28	82525.0
29	85612.5
30	88700.0
31	95204.5
32	101709.0
33	101709.0
34	110096.5
35	118484.0
36	128264.0
37	138044.0
38	143667.5
39	149291.0
40	149291.0
41	153792.0
42	158293.0
43	164419.0
44	170545.0
45	187021.5
46	203498.0
47	203498.0
48	277784.5
49	352071.0
50	319744.0
51	287417.0
52	254231.5
53	221046.0
54	221046.0
55	190091.5
56	159137.0
57	129791.0
58	100445.0
59	88195.0
60	75945.0
61	75945.0
62	66697.5
63	57450.0
64	49768.5
65	42087.0
66	36479.5
67	30872.0
68	30872.0
69	26360.0
70	21848.0
71	18929.0
72	16010.0
73	13316.5
74	10623.0
75	10623.0
76	8382.5
77	6142.0
78	4599.5
79	3057.0
80	2324.0
81	1591.0
82	1591.0
83	1180.5
84	770.0
85	605.5
86	441.0
87	315.0
88	189.0
89	189.0
90	135.0
91	81.0
92	53.0
93	25.0
94	16.0
95	7.0
96	7.0
97	6.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3056189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.73810435298947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93749829184418	28.983979183672954
2	13.837675469915686	10.99765983652624
3	5.232755110272488	6.2381930587694105
4	2.524951768693498	4.013471874824302
5	1.418851032855634	2.8191225202482033
6	0.8286821649094852	1.975815500678061
7	0.5555094146112682	1.545242376082347
8	0.4060236543293189	1.2907688276416478
9	0.27444918758598996	0.9815481410296726
>10	1.6784292133159295	12.703495512783109
>50	0.17420750400180696	4.814316432264912
>100	0.11482350966359713	8.495185699631126
>500	0.008196021138991778	2.160801026497077
>1k	0.0066230473850389425	5.492818650550656
>5k	4.967285538779206E-4	1.1224611413195607
>10k+	8.278809231298678E-4	6.365120217480758
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40757	1.3335889894244106	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38804	1.2696858734849186	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34539	1.1301329858853624	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14551	0.4761158423121083	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11754	0.38459663325795623	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10936	0.3578312728695771	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10831	0.35439562147498077	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10651	0.3485059333699585	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10424	0.34107838225973586	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10231	0.3347633277915731	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6347	0.20767694668098077	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6219	0.2034887240285205	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5797	0.1896806774711904	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5412	0.17708328902433718	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5248	0.17171712875087242	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5096	0.1667436143510758	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4847	0.15859621247246164	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4461	0.145966103536136	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4281	0.1400764154311137	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4086	0.13369591998400623	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3872	0.12669373523692415	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3827	0.1252213132106686	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3826	0.12518859272119623	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3665	0.11992059391614852	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3502	0.11458715413215609	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3204	0.10483644826939695	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3093	0.10120447393796653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2720489472346117E-5	0.0
3	3.2720489472346117E-5	0.0	0.0	3.2720489472346117E-5	0.0
4	3.2720489472346117E-5	0.0	0.0	3.2720489472346117E-5	0.0
5	3.2720489472346117E-5	0.0	0.0	3.2720489472346117E-5	0.0
6	6.544097894469223E-5	0.0	0.0	3.2720489472346117E-5	0.0
7	6.544097894469223E-5	0.0	0.0	6.544097894469223E-5	0.0
8	6.544097894469223E-5	0.0	0.0	6.544097894469223E-5	0.0
9	6.544097894469223E-5	0.0	0.0	1.6360244736173058E-4	0.0
10	6.544097894469223E-5	0.0	0.0	4.253663631404995E-4	0.0
11	6.544097894469223E-5	0.0	0.0	7.198507683916145E-4	0.0
12	6.544097894469223E-5	0.0	0.0	0.0010797761525874218	0.0
13	6.544097894469223E-5	0.0	0.0	0.0013742605578385368	0.0
14	1.3088195788938447E-4	0.0	0.0	0.0016033039841449597	0.0
15	1.3088195788938447E-4	0.0	0.0	0.0020941113262301515	0.0
16	1.6360244736173058E-4	0.0	0.0	0.003141166989345227	0.0
17	1.6360244736173058E-4	0.0	0.0	0.004286384120877341	0.0
18	1.6360244736173058E-4	0.0	0.0	0.005398880762937109	0.0
19	1.6360244736173058E-4	0.0	0.0	0.0069694642576097225	0.0
20	1.6360244736173058E-4	0.0	0.0	0.011484891804793486	0.0
21	1.6360244736173058E-4	0.0	0.0	0.018552517530820246	0.0
22	1.6360244736173058E-4	0.0	0.0	0.02948116101458385	0.0
23	1.6360244736173058E-4	0.0	0.0	0.035861656461691344	0.0
24	1.6360244736173058E-4	0.0	0.0	0.04574324428233987	0.0
25	1.6360244736173058E-4	0.0	0.0	0.0526472675610049	0.0
26	1.963229368340767E-4	0.0	0.0	0.06432848230263247	0.0
27	1.963229368340767E-4	0.0	0.0	0.14832197877814493	0.0
28	1.963229368340767E-4	0.0	0.0	0.2584918668315343	0.0
29	1.963229368340767E-4	0.0	0.0	0.3835168571053688	0.0
30	2.2904342630642282E-4	0.0	0.0	0.5953820264388099	0.0
31	2.2904342630642282E-4	0.0	0.0	0.8964759705633388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9765	0.0	29.459806	1
CTCGCGA	45	0.0038272378	20.555557	14
ACGTTTA	145	0.0	20.413794	26
GTATTGG	950	0.0	20.057894	1
ATTGGAC	1005	0.0	18.960197	3
TTGGACC	1765	0.0	17.399433	4
GTATCAA	16920	0.0	17.111406	2
GGACCCT	1855	0.0	16.256065	6
ACCGGAT	70	0.002593814	15.857142	8
GTATAAC	200	3.6379788E-12	15.725	1
TCACGTT	200	3.6379788E-12	15.725	24
TGGACCC	2010	0.0	15.646766	5
CCGACCG	255	0.0	15.235294	9
TATACCG	240	0.0	14.645833	5
GACCCTC	2130	0.0	14.591549	7
CGTATAC	115	2.2121063E-5	14.478261	3
TACGTTA	205	9.276846E-11	14.439025	19
CGTGTCT	340	0.0	14.147059	35
CGACCGC	250	0.0	14.059999	10
TTTTTAC	2055	0.0	13.863747	1
>>END_MODULE