FastQCFastQC Report
Wed 25 May 2016
SRR2088340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064073
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT411571.3432121232098582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT393101.2829328805155753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT348231.1364938106892362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141310.46118352924359174No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG116020.3786463312068609No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA113370.36999771219549926No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA112330.36660353718726674No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG112220.36624453790754985No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG108990.35570301360313544No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA102040.3330207863846586No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA69780.2277360885331387No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA62360.20351995530132602No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC59210.19323952138216027No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58120.18968216488314738No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.17052465786552734No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA51140.16690202877020227No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA50700.1654660316513347No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG45200.1475160676654897No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG44570.14545998088165654No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG41430.13521218326064685No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39630.12933764959255212No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39160.12780374357921628No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC37480.12232084548899455No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36910.12046057649409789No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG35330.11530404138543697No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC34970.11412913465181802No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32050.10459933559024213No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT30890.10081352500413666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98350.029.6639561
ATTGGAC10200.023.5784323
GTATTGG10050.023.3781091
TTGGACC18050.019.1662044
TGGACCC18600.018.6989255
GGACCCT18100.018.602216
ACGTTTA1950.018.02564226
GACCCTC18750.017.5626687
TATACCG2550.017.4117645
GTATCAA171150.017.1326332
CAATGCG2200.016.81818219
TATTGGA14750.016.3050842
GTAGTGT1751.3278623E-1015.8571436
CTGTGCG2800.015.8571429
CGTGTCT3450.015.55072535
CGACGAG2500.015.54000124
GACCGTG2650.015.3584917
CGAATTA2650.015.35849115
CGGTATA2300.015.28260726
CCGACCG2800.015.1964289
Produced by FastQC (version 0.11.5)